Method using tissue-subculture sprouts of oil-tea camellia to raise seedlings by grafting

Abstract

The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seed storage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-tea camellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.

Description

Technical field: [0001] The invention belongs to the technical field of plant asexual rapid propagation, and relates to a method for growing seedlings of camellia oleifera through tissue culture and subgeneration by grafting. Background technique: [0002] Camellia oleifera (Camellia oleifera Abel) is a plant of the genus Camellia in the family Theacae. Camellia oleifera seeds have high oil content. It is a unique edible oil tree species in my country. It is also known as the world's four largest edible woody oil tree species together with olive, oil palm and coconut. , with the camellia oil extracted from its seeds, the unsaturated fatty acid content of oleic acid and linoleic acid accounts for more than 90%, which is comparable to olive oil, and is known as "Oriental olive oil". There is no cholesterol in camellia oil, it will not increase the cholesterol in human serum after eating, it is not easy to cause hardening of human blood vessels and increase blood pressure, and i...

AI Technical Summary

Problems solved by technology

Its shortcomings are: 1. The good and bad trees are uneven, the yield is low, and the yield of tea oil per mu is only 3 to 4 kilograms; 2. The fruit is late. After transplanting and afforestation, it takes 8 years to bear fruit, and it can be harvested after 10 o'clock, and it takes more than 15 years. 3. Weak nature, mainly anthracnose, soft rot and bituminous coal disease; 4. It is difficult to propagate seedlings, and the survival rate of direct seeding propagation is only about 20%.

However, the existing methods of vegetative propagation of Camellia oleifera, such as cutting propagation, seedling cultivation by cutting leaves and grafting of bud seedlings, require a large number of buds of good varieties. Because the buds of good varieties are limited, it is difficult to meet the needs of the development of the national Camellia oleifera industry for excellent clones of Camellia oleifera. needs

In order to overcome the poor quality of direct seeding and the lack of reproduction technology that requires a large number of fine buds in the existing asexual propagation method, the method of tissue culture subculture bud grafting and seedling cultivation of the present invention is a good method for rapid asexual propagation of Camellia oleifera quality seedlings