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Method for culturing detoxified seedling by sweet potato stem tip

A technology of virus-free seedlings and stem tips, which is applied in the field of plant cultivation, can solve the problems of small sweet potato stem cutting propagation, poor virus detoxification degree, and low shoot tip seedling rate, so as to improve the shoot tip seedling rate , short multiplication period and high seedling quality

Inactive Publication Date: 2009-11-25
INST OF FOOD CROPS HAINAN ACAD OF AGRI SCI
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Problems solved by technology

[0003] At present, the virus-free sweet potato is generally cultivated by peeling off the stem tip, and then carrying out stem cutting and multiplication. There are two problems that need to be solved in depth: one is the poor degree of virus-free virus, and the shoot-tip seedling rate is low; Second, the amount of multiplication of sweet potato stem cuttings is small and the cost is high

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  • Method for culturing detoxified seedling by sweet potato stem tip

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Embodiment Construction

[0025] The present invention will be further described below.

[0026] A method for cultivating virus-free seedlings of sweet potato stem tips, the specific steps are as follows:

[0027] 1. Seed potato seedling cultivation

[0028] Select high-yield and high-quality potato chips suitable for local cultivation, soak the seeds in warm water at 50-54°C for disinfection, and then plant them in the ground until the seedlings grow to 25-30cm.

[0029] 2. Sterilization of materials

[0030] Cut off the top 3-5cm of the shoot tip of the seedlings, cut off the larger leaves, rinse with running water for several minutes, dry the surface water with filter paper, and then carry out disinfection treatment.

[0031] 3. Peel off the stem tip

[0032] Peel off the leaves of the sterilized shoot tip, cut out the shoot tip meristem of 0.3-0.5mm, containing 1-2 leaf primordia, and inoculate it in the shoot tip meristem culture medium. The shoot apex meristem culture medium is based on MS, a...

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Abstract

The invention relates to plant cultivation field, particularly a method for culturing the detoxified seedling by the sweet potato stem tip. The method comprises following steps: firstly selecting qualified potato seed for raising seedling, scissoring the photo seedling stem tip, peeling the stem tip meristem for culturing, seedling formation cultivation after the stem tip meristem enlarges and turns green, performing the dwarf shoot cottage cultivation to get the test-tube plantlet, virus detection, taking the detoxified seedling leaves for inducement rapid propagation, domestication and transplantation, obtaining the stem tip seedling of the breeder's seed potato. The invention has simple art, utilizes the detoxified seedling leaves inducement rapid propagation technology to obtain multiple detoxified seedlings of one strain in a short period so as to lower production cost, effectively improve the virus detoxified rate and stem tip establishing percent and rapidly provide the nontoxic seedling for the sweet potato production and resource storage.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for cultivating virus-free seedlings from sweet potato stem tips. Background technique [0002] Sweet potato (Ipomoea batatas Lam.) is an important food, feed and industrial raw material crop, which has the advantages of high yield, rich nutrition and good yield stability. However, due to the widespread existence of sweet potato virus diseases and the vegetative propagation of sweet potatoes in tubers during production, the virus diseases spread, resulting in reduced yield, lower quality and degeneration of sweet potato species. In view of the fact that no practical sweet potato varieties with high resistance to viral diseases have been bred so far at home and abroad, and there is no high-efficiency pesticide for preventing and controlling viral diseases. The only effective way to degrade and improve the yield and quality of sweet potatoes. [0003] At present, the vir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘迪朱红林白翠云孟卫东
Owner INST OF FOOD CROPS HAINAN ACAD OF AGRI SCI
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