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Method for removing Leifsonia xyli subsp.xyli and quickly breeding health cane seedling

A technology of sugarcane and germs, applied in the field of reproduction and production of sugarcane seedlings, can solve the problems of high phenol pollution rate, easy damage to sugarcane buds, low seedling reproduction coefficient, etc., achieve the removal of RSD germs, improve drought resistance, and facilitate transportation Effect

Inactive Publication Date: 2009-02-11
GUANGZHOU SUGARCANE IND RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The early traditional method of removing RSD bacteria from sugarcane is soaking seeds in hot water at 50°C for 2-3 hours or treating sugarcane seeds with hot air at 54-58°C for 8 hours, which can kill RSD pathogens in susceptible sugarcane stems to a certain extent and greatly reduce RSD. However, the above-mentioned heat treatment method has the disadvantages of not being able to completely remove RSD germs, easily damaging sugarcane buds, difficult to control water temperature, unstable degerming effect, and low seedling reproduction coefficient. Large-scale promotion and application in production
In recent years, there have also been reports on the use of heat treatment combined with shoot apical meristem (less than 1 mm) culture to remove sugarcane RSD pathogens, but the explants cut by this method are very small and need to be cut under a dissecting microscope, which is difficult to operate, and due to the The body is very small, resulting in a high rate of phenol pollution during the cultivation process, an extremely low rate of induced seedling formation, and high production costs, making it difficult to popularize and apply in production

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for efficiently removing sugarcane perennial dwarf pathogens and rapidly propagating healthy sugarcane seedlings, comprising the steps of:

[0026] 1. Processing and production of original seeds: Take 10 main stems of sugarcane variety Yuetang 00-236 (plant age 8 months) in mid-August, cut them into double-bud segments, and select 40 effective bud segments with better quality cane buds , treated in a water bath at 50±0.3°C for 2 hours, placed in a light incubator at 35°C for 3 days to accelerate germination, and after sprouting, grow seedlings with fine sand in a glass greenhouse (28-30°C) until the seedlings grow into 3-4 pieces When the leaves and pseudostems were 25 cm high, they were transplanted in the net room of the test base. When the sugarcane grew to 12 to 13 buds, 20 normal growing sugarcane stems (reserved cane tip), and number each cane stalk, cut off one of the 1-4 nodes at the base of each cane stalk, extract 200-300 μL of juice with an air pump,...

Embodiment 2

[0036] A method for efficiently removing sugarcane perennial dwarf pathogens and rapidly propagating healthy sugarcane seedlings, comprising the steps of:

[0037]1. Processing and production of original seeds: From August to September, 10 main stems of sugarcane variety Xintaitang No. 22 (plant age 8 months) were taken, cut into double buds, and 32 effective buds with better quality cane buds were selected. The double-bud section was treated in a water bath at 50±0.3°C for 2.5 hours, and placed in a light incubator at 37°C for 4 days to accelerate germination. After the cane sprouts germinated, they were raised in a glass greenhouse (28-30°C) with fine sand until the cane seedlings grew. When 3 to 4 leaves and a pseudostem height of 26 cm were transplanted in a net room nursery, about 10 buds grew from the sugarcane, and 20 normal sugarcane stems were cut with a cane knife soaked in 75% wine for more than 5 minutes ( Keep the sugarcane tips), and number each cane stem, cut of...

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PUM

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Abstract

The invention relates to the propagation production of cane seedlings, and provides a method for removing cane perennial root dwarfing germs and rapidly propagating healthy cane seedlings, which comprises the following steps: little amount of original seeds are treated by hot water, stem tips which are detected to be feminine by the regenerated plantlet by carrying out speckle enzyme marker immune test (DB-EIA) is taken as an explant, healthy seedlings are produced by induction culture, secondary propagation culture, root-promoting culture, temporary planting and second-stage or third-stage nursery propagation-extension; furthermore, the disinfection of seed-cutting tools (cane knives) is paid attention to in the nursery propagation-extension, before the seedlings are used as seeds for large field production after coming out from nursery, cane stem juice is extracted by sampling, and RSD virus is detected by using the DB-EIA, thus guaranteeing the providing of qualified seedlings. The method has the advantages that: dwarfing disease pathogenic bacteria of the perennial root of the cane seedling can be thoroughly removed, annually propagation coefficient can reach as high as more than 10 thousand times, furthermore, the seedling has no variation, has the advantages of being fast, high-efficient and safe, and is suitable for the industrialized and standardized production of the cane healthy seedlings on scale.

Description

technical field [0001] The present invention relates to the reproduction and production of sugarcane seedlings, in particular to the technology for removing dwarfing pathogens of sugarcane perennial roots Background technique [0002] Sugarcane (Saccharum.spp) is the main sugar crop and a biomass crop with good development prospects in my country. Sugarcane ratoon dwarf disease (RSD) is a worldwide important sugarcane disease, and it is also a major sugarcane disease in my country's sugarcane regions. 15%, the loss of ratoon cane yield is 20% to 25%, and affects the life of rattan cane, which is one of the important reasons for the degradation of sugarcane varieties. The pathogenic bacteria of sugarcane RSD live in the vascular bundles of sugarcane stems, and there is no effective chemical control method; at the same time, due to the lack of resistant source materials, sugarcane RSD disease-resistant breeding has not achieved much, and no production varieties with better res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 沈万宽陈仲华邓海华刘睿何慧怡杨湛端
Owner GUANGZHOU SUGARCANE IND RES INST
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