Method for removing Leifsonia xyli subsp.xyli and quickly breeding health cane seedling
A technology of sugarcane and germs, applied in the field of reproduction and production of sugarcane seedlings, can solve the problems of high phenol pollution rate, easy damage to sugarcane buds, low seedling reproduction coefficient, etc., achieve the removal of RSD germs, improve drought resistance, and facilitate transportation Effect
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Embodiment 1
[0025] A method for efficiently removing sugarcane perennial dwarf pathogens and rapidly propagating healthy sugarcane seedlings, comprising the steps of:
[0026] 1. Processing and production of original seeds: Take 10 main stems of sugarcane variety Yuetang 00-236 (plant age 8 months) in mid-August, cut them into double-bud segments, and select 40 effective bud segments with better quality cane buds , treated in a water bath at 50±0.3°C for 2 hours, placed in a light incubator at 35°C for 3 days to accelerate germination, and after sprouting, grow seedlings with fine sand in a glass greenhouse (28-30°C) until the seedlings grow into 3-4 pieces When the leaves and pseudostems were 25 cm high, they were transplanted in the net room of the test base. When the sugarcane grew to 12 to 13 buds, 20 normal growing sugarcane stems (reserved cane tip), and number each cane stalk, cut off one of the 1-4 nodes at the base of each cane stalk, extract 200-300 μL of juice with an air pump,...
Embodiment 2
[0036] A method for efficiently removing sugarcane perennial dwarf pathogens and rapidly propagating healthy sugarcane seedlings, comprising the steps of:
[0037]1. Processing and production of original seeds: From August to September, 10 main stems of sugarcane variety Xintaitang No. 22 (plant age 8 months) were taken, cut into double buds, and 32 effective buds with better quality cane buds were selected. The double-bud section was treated in a water bath at 50±0.3°C for 2.5 hours, and placed in a light incubator at 37°C for 4 days to accelerate germination. After the cane sprouts germinated, they were raised in a glass greenhouse (28-30°C) with fine sand until the cane seedlings grew. When 3 to 4 leaves and a pseudostem height of 26 cm were transplanted in a net room nursery, about 10 buds grew from the sugarcane, and 20 normal sugarcane stems were cut with a cane knife soaked in 75% wine for more than 5 minutes ( Keep the sugarcane tips), and number each cane stem, cut of...
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