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Non-tube cutting seedling method of tissue subcultured buds of zenia

A technology for raising seedlings by cuttings and subsequent buds, which is applied to the field of seedling raising by cuttings outside of a bean tissue culture subsequent sprout bottle, can solve the problems of consuming more manpower and material resources, high cost, complicated procedures and the like, and achieves shortening the time for raising seedlings, shortening the cycle and simplifying the process. The effect of the process

Inactive Publication Date: 2012-12-19
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after subculturing in traditional tissue culture, a step of rooting culture is required to allow the subcultured shoots to take root in the culture medium in the bottle, and then transplanted to the matrix. The procedure is complicated, and the rooting culture in the bottle Strict aseptic operation is required to ensure the quality of seedlings, and the cost is relatively high. In addition, transplanting culture requires more manpower and material resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Select the young shoots with axillary buds in the middle and upper part of the canopy of robust and disease-free Rendou plants as explants, sterilize and inoculate them into sterile medium for bud induction culture, and transfer the sterile buds to the successor after obtaining sterile buds. subculture on subculture medium. The cuttings were carried out in October, and the red heart soil was selected as the substrate, crushed by a soil crusher, passed through an 80-90 mesh sieve, put into a plastic cup with a depth of 7×9cm and 10cm deep, placed on the seedling bed, and used 3g / L before cutting. Potassium permanganate solution for disinfection for 24 hours. After disinfection, clear water drenches the matrix and cleans the medicine; selects the secondary shoots that grow vigorously and have a height ≥ 5cm as spikes. After washing away the medium, cut them at 3mm below the terminal node, Soak the ends of the cuttings in MS liquid medium containing 0.3 mg / L ABT6, the soak...

Embodiment 2

[0030] Select the young shoots with axillary buds in the middle and upper part of the canopy of robust and disease-free Rendou plants as explants, sterilize and inoculate them into sterile medium for bud induction culture, and transfer the sterile buds to the successor after obtaining sterile buds. subculture on subculture medium. Cuttings were carried out in March, and red heart soil was selected as the substrate, crushed by a soil crusher, passed through a 90-100 mesh sieve, put into a plastic cup with a depth of 7×9cm and 10cm deep, placed on a seedling bed, and used 5g / L before cutting Potassium permanganate solution was used for disinfection for 36 hours. After disinfection, clear water drenched the matrix and cleaned the medicine; selected the vigorously growing and high ≥ 5cm secondary shoots as spikes, washed away the medium, and cut them at 3mm below the terminal node. The end of the cuttings is soaked in MS liquid medium containing 0.4mg / L ABT6. The soaked part accou...

Embodiment 3

[0032]Select the young shoots with axillary buds in the middle and upper part of the canopy of robust and disease-free Rendou plants as explants, sterilize and inoculate them into sterile medium for bud induction culture, and transfer the sterile buds to the successor after obtaining sterile buds. subculture on subculture medium. The cuttings were carried out in April, and the red heart soil was selected as the substrate, crushed by a soil crusher, passed through a 80-100 mesh sieve, put into a plastic cup with a depth of 7×9cm and 10cm deep, placed on the seedling bed, and used 5g / L before cutting Potassium permanganate solution for disinfection for 24 hours. After disinfection, clear water drenches the substrate and cleans the drug; selects the vigorous growth and the secondary buds with a height ≥ 5cm as spikes, washes off the medium, and cuts them at 3mm below the terminal node. The end of the cuttings is soaked in MS liquid medium containing 0.5mg / L ABT6. The soaked part ...

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Abstract

The invention discloses a non-tube cutting seedling method of tissue subcultured buds of zenia. The two steps of non-tube rooting of tissue subcultured buds and cutting seedling are combined into one. The tissue subcultured buds of zenia are bred by a conventional method, and then the tissue subcultured buds of zenia are selected as cuttings and are treated by a root promoter and cut into a red subsoil matrix, and seedlings are cultured to obtain forestation wood seedlings for planting. According to the method, the tissue subcultured buds of zenia are directly used as the cuttings for non-tube cutting, the breeding speed is fast, and seasonal and geographical restrictions do not exist; and meanwhile, the two steps of non-tube rooting and cutting seedling are combined into one, the method is simple, the operation is easy, rooting starts after cutting culture for 15 to 20 days, the seedling survival rate is high, and the rooting survival rate is 85% to 95%. The method overcomes the defects of the traditional zenia asexual reproduction method, the seedling cycle is shortened, the seedling survival rate is improved, the seedling cost is reduced, and the method is favorable for accelerating the development of asexual seedling of zenia.

Description

technical field [0001] The invention belongs to the technical field of asexual rapid propagation of plants, and relates to a method for growing seedlings by subcultured buds, in particular to a method for growing seedlings by cuttings outside a bottle for tissue cultured subcultured buds of Rendou bean. Background technique [0002] Ren bean (Zenia insignis), also known as winged viburnum, is a deciduous tree of the Hematoxylin family. It is a unique tree species in my country, and it is a rare tree species. Rendou has the characteristics of rapid growth, strong germination, strong root penetration, and strong adaptability. It is distributed in Guangxi, Guangdong, Yunnan, Hunan, and Guizhou provinces (regions) between the middle subtropical zone and the southern subtropical zone. It is an important tree species in the construction of ecological public welfare forests in South China, and it has become one of the preferred native tree species for afforestation and vegetation r...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 陈博雯蔡玲刘海龙陈晓明覃子海王鹏良
Owner GUANGXI FORESTRY RES INST
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