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Gardenia jasminoides tissue cultivation and non-pollution cultivation method thereof

A technology for tissue cultivation and planting methods, which is applied in the directions of botanical equipment and methods, horticultural methods, cultivation, etc., to achieve the effect of overcoming pesticide and heavy metal pollution

Inactive Publication Date: 2012-04-11
董永武
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are only a small number of reports on the germplasm resources of the genus Hosta and garden applications, and the tissue culture of gardenia species for ornamental use in various domestic literature, but there is no report on the tissue culture and planting of gardenia. to report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Collection and processing of explant materials:

[0019] Select the new leaves and young leaves of the mother plant that grows lush and disease-free in June as explant materials, collect the whole plant of wild or domestic gardenia and transplant it in the flower box, wash the leaves with water, and wait for the new leaves After growing, cut the living leaves from the mother plant and rinse them with tap water for 15 minutes to remove the dust and fluff on the surface of the leaves. Put the leaves in 70% ethanol and turn over for 20 seconds. After removing the waxy substances on the surface of the leaves, rinse with water. Remove the ethanol from the leaves, soak them in 0.1% mercuric solution for 15 minutes for disinfection, wash them with sterile water and cut them into 1cm 2 Small squares, the back of the leaf is placed flat in the medium, 4 explants are placed in each bottle, the bottle cap is tightly closed, the bottle mouth is sealed with a fresh-keeping bag, a...

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Abstract

The invention relates to a method for cultivating gardenia jasminoides, which belongs to the field of plant tissue culture, in particular to a method for cultivating the gardenia jasminoides and a non-pollution cultivation method thereof. The method comprises the following steps: disinfecting and cleaning the outer planting body of the gardenia jasminoides; inducing and differentiating the callus; proliferating the adventitious buds; inducing the adventitious roots; acclimatizing; and executing the non-pollution cultivation to realize the experiment purpose of the invention. The invention has the following beneficial effects: the invention obtains a large number of tissue culture seedlings to meet the planting needs, and overcomes the pollutions of pesticide and heavy metal in natural wood area. The survival rate is 95.5%, the weight of single plant is twice as the wild and asexual reproduction drug materials in the same year, and the effective components is higher than the wild drug materials in the same year. The invention meets the needs of original ingredients of pharmaceutical industries, not only cultivates in batches, but also plants the tissue culture seedlings in all areas, obtains the non-pollution drug materials two years in advance, and has practical value.

Description

technical field [0001] The invention relates to a gardenia tissue culture method, belonging to the field of plant tissue culture, in particular to a gardenia tissue culture and a pollution-free planting method. Background technique [0002] Gardenia, also known as Gardenia, is an evergreen shrub of Gentiana, Rubiaceae, and Gardenia. It likes a warm, humid and sunny environment. It is cold-resistant, half-shade-resistant, afraid of water accumulation, and requires looseness, fertility and acidity. The sandy loam is native to China. Its flowers and branches are lush, the leaves are evergreen in all seasons, and the flowers are fragrant and elegant. In addition to ornamental purposes, its flowers, fruits, leaves and roots can be used as medicine, which has the functions of purging fire and eliminating vexation, clearing heat and diuretic, cooling blood and detoxifying. It is widely used all over the world as a garden ornamental plant and a medicinal plant. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G9/10
Inventor 董永武
Owner 董永武
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