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Cyst inactivation method for controlling cryptocaryon irritans disease of fishes

A technology that stimulates cryptonium disease and cysts, and is applied in fish farming, applications, climate change adaptation, etc., and can solve problems such as drug residues, fish toxicity, and environmental pollution.

Inactive Publication Date: 2016-06-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current methods commonly used to prevent and treat cryptocystosis are still frequent use of chemical drugs such as formaldehyde, CuSO4, and copper-iron mixture. Although chemical drugs can effectively control cryptocystosis, there are huge hidden dangers in practical applications, such as Fish toxicity, drug residues, environmental pollution, etc., so an environmentally friendly treatment method is imminent

Method used

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  • Cyst inactivation method for controlling cryptocaryon irritans disease of fishes
  • Cyst inactivation method for controlling cryptocaryon irritans disease of fishes
  • Cyst inactivation method for controlling cryptocaryon irritans disease of fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: The effect of treating the disease by removing cysts of Cryptocaryon stimuli

[0019] In this experiment, the red pepper seabream (27.79±3.07g / tail) was purchased from Auda Bay, Sandu, Ningde City. Before the experiment, they were raised for two weeks in a circular plastic bucket (1 ton in volume), raised in micro-flow water, and fed pellets. The seawater was natural seawater with a salinity of 29-32%, dissolved oxygen ≥ 5.0mg / l, and pH7. 9~8.3, nitrite <0.1mgN / l.

[0020] The pepper seabream were randomly divided into three groups: control group, infection group, padding group, each group had three replicate groups, and each replicate group had 30 fish. Among them, the infection group and the pad group were infected with 30 larvae per fish with 3000 larvae / fish, the amount of seawater was 5L / fish, and the running water was turned on after 2 hours of infection. The control group and the infection group were cultured normally, and the pad cloth group was exp...

Embodiment 2

[0022] Make the pad cloth into a size of 5cm×5cm, artificially infect 20 capricorn breams (27.79±3.07g / tail) with 3000 larvae / fish, and place the prepared pad cloth on the third day to infect and stimulate Cryptocaryonia At the bottom of the fish tank, take out the cloth on the 4th day, and dry the cysts attached to the cloth at room temperature, and set up 5 groups of drying time: 10min, 30min, 60min, 120min, 240min, After drying, put them into seawater at 27°C for incubation, observe the number of empty cysts through a stereoscope, and count the hatching within 144 hours. The experimental results showed that when the cysts were dried for 120 minutes, the hatching rate of the cysts was zero.

Embodiment 3

[0024] Collect the cysts that fall off within 24 hours and put them in a beaker, add 20ml of seawater to the 100ml beaker and heat it in a water bath. When the seawater in the beaker reaches the test temperature, absorb about 100 cysts and add them to the beaker for 1min and 3min respectively Finally, take out the beaker and add 80ml of normal temperature seawater to it, transfer the cysts to a 24-well plate, let it incubate at 27°C, count the hatching rate within 144 hours, and set three parallels for each gradient. The results showed that treating the cysts at 43°C for 3 minutes and at 48°C for 1 minute could kill the cysts 100%.

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Abstract

The present invention discloses a cyst inactivation method for controlling the cryptocaryon irritans disease of fishes. Mainly through screening a cloth liner having a strong adhesion effect on cryptocaryon irritans cysts, laying the cloth liner to the water bottom of the fishes infected by the cryptocaryon irritans disease so as to enable trophozoites of the cryptocaryon irritans to be adhered on the cloth liner after falling off, and removing the cloth liner to remove the cryptocaryon irritans cysts, life history of the cryptocaryon irritans is cut off, and death of the fishes caused by repeated infection of the cryptocaryon irritans is reduced. According to the method, the cloth liner which is corrosion resisting and nontoxic and has a strong adhesion effect on the cysts is obtained through screening materials, plectorhinchus cinctus infected by the cryptocaryon irritans disease is treated by using the method of removing the cryptocaryon irritans cysts, and relative protection ratio can reach 98%. Drying treatment and heat treatment are carried out on the cysts, and the results show that the cysts can be 100% inactivated when the cysts are dried for 120 min, and the cysts can be 100% inactivated when the cysts are processed for 3 min at 43 DEG C or processed for 1 min at 48 DEG C.

Description

technical field [0001] The invention belongs to the field of fish disease prevention and control, and in particular relates to a cyst inactivation method for controlling fish irritating cryptocystosis. Background technique [0002] According to the present invention, Cryptocaryon stimuli cysts have strong adhesion, and it takes at least three days for the cysts to escape from the formation of larvae. Lay pads at the bottom of fish ponds infected with Cryptocaryon stimuli, replace them regularly, and cut off the cysts. Life history, inactivation of cysts on the pads, in order to achieve the purpose of controlling irritating Cryptocaryoniasis. [0003] Cryptocaryon irritatus is a ciliate widely parasitic on bony fish in tropical and subtropical seawater. Because white spots visible to the naked eye will appear on the infected body surface, fin rays, and gills of fish, it is named "white spot disease". Cryptocaryon stimuli has no intermediate host, and its life history is divi...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 李安兴江飚
Owner SUN YAT SEN UNIV
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