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Hybrid between longtooth grouper Epinephelus bruneus and giant grouper Epinephelus lanceolatus and a hybridization method thereof

A kind of saddle grouper, production method technology, applied in fish farming, application, climate change adaptation and other directions, can solve the problem of not realizing hybridization with subtropical sushi, and achieve the effect of improving national competitiveness

Active Publication Date: 2017-08-11
KOREA INST OF OCEAN SCI & TECH +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, until now, the crossbreeding of genusidae has only been between subtropical fish species, and the hybridization between genusidae and subtropical genusidae that inhabits Korea has not been realized.

Method used

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  • Hybrid between longtooth grouper Epinephelus bruneus and giant grouper Epinephelus lanceolatus and a hybridization method thereof
  • Hybrid between longtooth grouper Epinephelus bruneus and giant grouper Epinephelus lanceolatus and a hybridization method thereof
  • Hybrid between longtooth grouper Epinephelus bruneus and giant grouper Epinephelus lanceolatus and a hybridization method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Ensuring of broodstock

[0046] In the present invention, as the brown grouper, a mature female brown grouper collected near Jeju, Korea and domesticated in a broodstock breeding tank with a body weight of 1.5 to 5.2 kg was used. , matured male grouper with a body weight of 54.2-112.0 kg imported from Taiwan and being domesticated in a broodstock breeding tank was used.

Embodiment 2

[0047] Example 2. Hybridization

[0048] Hybridization was carried out by artificial insemination using the dry method after extracting eggs and semen from mature female brown grouper and male saddle grouper by abdominal compression method.

[0049] First, in order to obtain high-quality mature eggs of mature female brown grouper, after observing the abdomen, the state of maturity was checked while touching with hands.

[0050] 25 μg / kg of luteinizing hormone-releasing hormone (LHRH) (Sigma, USA) and 1000 IU / kg of human chorionic gonadotropin (HCG) were intraperitoneally injected into the maturing female brown grouper.

[0051] From 36 hours after the injection, the mature eggs were checked by hand at intervals of 4 hours, and finally, the mature eggs were secured by compressing the abdomen.

[0052] Semen was extracted from male saddle grouper using abdominal compression.

[0053] It is necessary to prevent the ensured mature eggs from being exposed at room temperature for ...

Embodiment 3

[0057] Embodiment 3. Hatching and raising

[0058] Hatching and rearing of larvae were performed in the same manner in the hatching tank.

[0059] The results of the hatching experiment showed that all hatchings were carried out at the water temperature of the hatch tank up to 22-31°C, but the hatching rates at 22°C and 31°C were lower than those at 25°C and 28°C.

[0060] The survival rate of yolk-absorbed larvae showed high values ​​in the 25°C and 28°C experimental groups, but did not survive in the 22°C and 31°C experimental groups ( figure 1 ).

[0061] The results of the analysis of the deformity rate of hatched larvae show that at a temperature of 25°C, there is a deformity rate of 1.5%, while at a temperature of 28°C, the deformity rate is as high as 15.5%. It is 23.5-26.5°C.

[0062] Rotifers (rotifers) were fed from 2 days after hatching.

[0063] As the larvae grow, a compound feed is provided together with the Artemia larvae. The rearing water used filtered se...

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Abstract

The present invention relates to hybrids of brown grouper and grouper and their production methods, and more particularly to the use of two species of brown grouper and grouper in two families, and hybrids between the male brown grouper and the male grouper grouper and the hybrid of the male brown grouper and the female grouper grouper production method. The present invention can provide hybrids with high growth rate and excellent quality through the hybridization between highly marketed brown grouper and fast-growing and commercially available grouper grouper. The growth of the initial larvae in the hybrids of the present invention is so fast as to solve the problem of biological bait that becomes a major problem when breeding fish species, thereby contributing to the activation of the Korean aquaculture industry.

Description

technical field [0001] The present invention relates to a hybrid produced by crossing between two species of brown grouper and saddle grouper belonging to the grouper subfamily in the family Anguidae and a method for producing the hybrid, and relates to a female brown grouper (longtooth grouper, Epinephelus bruneus) and male saddle grouper (giant grouper, Epinephelus lanceolatus) hybrids and methods for producing hybrids of male brown grouper and female saddle grouper. [0002] The present invention is a method comprising the steps of securing mature eggs from females, extracting semen from males, performing artificial insemination between the two to produce fertilized eggs, and raising the fertilized eggs to hatch and seedlings. [0003] The hybrid between the brown grouper and saddle grouper has the advantage of growing very fast compared to the brown grouper. Background technique [0004] The family Serranidae has 529 species in 3 subfamilies, of which the main species f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/00Y02A40/81
Inventor 卢忠焕金菫植崔纹祯朴栖轮黄效伸房仁哲韩尚峯文信柱林汉奎郑泰赫尹乐进
Owner KOREA INST OF OCEAN SCI & TECH
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