30 results about "Transgelin Gene" patented technology

The invention provides, in part, methods for the production of proteins in a transgenic non-human mammal, wherein the proteins are transported from the blood to the mammary gland for secretion in milk. The transport of the protein to the mammary gland and / or milk is facilitated by binding to a transport receptor in the mammary gland.

The invention provides immunochromatography test paper assembled by taking a chitin / chitosan fiber membrane as a colloid label pad and a preparation method thereof. The test paper has the advantages of uniform adsorption of negative-charge colloid particles, hydrophilic steady flow, neutral biomacromolecules, stable test, high sensitivity, long useful life and the like. In the colloid label pad, pure chitin fiber and deacylated chitosan fiber are mixed in any ratio, and a fiber membrane with thickness of 0.2-4mm is prepared by a pulping method, spinning method or solution method; the test paper which can load multiple kinds of colloid particle markers and are assembled with different detection membranes can be widely applied to the fields such as physical health, disease detection, drug and doping detection and transgenic protein detection.

The invention discloses a g10-epsps quantitative detection kit including the following components: I, an antibody pre-coated ELISA plate; II, a sample diluent which is a phosphate buffer solution; III, a washing liquid which is a phosphate buffer solution containing Tween-20; IV, a reaction stop liquid which is a H2SO4 solution; V, an enzyme labeled antibody which is a mouse anti g10-epsps monoclonal antibody labeled by horseradish peroxidase; VI, a color developing agent; and VII, a g10-epsps standard substance. The invention also discloses a method for quantitative detection of a transgenic protein g10-epsps in a plant by using the kit, wherein the method includes the following steps: extracting a protein from a to-be-tested sample, to obtain a protein extract liquid; using the g10-epsps quantitative detection kit for detecting the protein extract liquid; and making a concentration-absorbance standard curve by using g10-epsps protein standard substances, and calculating the g10-epsps protein concentration in the to-be-tested sample.

A plant expressing a cytokine and an autoantigen is disclosed. An example of a cytokine is a contra-inflammatory cytokine, for example IL-4 and IL-10, and an example of autoantigen is GAD. A novel method for the production of transgenic proteins of interest suitable for oral administration is also disclosed. Further, non-food crop plants expressing one or more proteins of interest are disclosed, as is a method involving the preparation of a protein of interest suitable for oral administration within a non-food crop plant comprising, transforming the non-food crop plant with a suitable vector containing a gene of interest and appropriate regulatory regions to ensure expression of the gene of interest within the non-food crop plant, such that the non-food crop plant is characterized as being non-toxic, non-addictive, palatable, and requiring minimal or no processing prior to oral administration. An example of a non-food crop plant is low alkaloid tobacco. Proteins of interest may include pharmaceutically active proteins such as growth regulators, insulin, interferons, interleukins, growth hormone, erythropoietin, G-CSF, GM-CSF, hPG-CSF, M-CSF, Factor VIII, Factor IX, tPA, antibodies, antigens and combinations and / or derivatives thereof.

The present invention discloses one kind of rockfish sex reversal relative gene sequence, vector and recombinant strain containing the gene and their use. The gene has cDNA of 608 bp length, one open reading frame of 252 bp (54-305), 83 coded amino acids, 5' non-coding region of 53 bp length and starting at one ANNATG motif included inside vertebrate initiator codon, and 3' non-coding region of 303 bp length and with polypeptide tailing signal (AAUAAA) and polypeptide tail. The vector and recombinant strain containing the gene and protoyon expressed fusion protein are used in propagation regulation, sex gland differentiation and sex conversion of rockfish and the feed additive preparation.

The invention relates to the technical field of biochemical detection, in particular to a test strip, a preparation method of the test strip and application of the test strip in transgenic protein AM79 EPSPS detection. The handheld colloidal gold rapid test paper for the transgenic protein AM79 EPSPS provided by the invention can be widely applied to rapid detection of the transgenic protein AM79EPSPS, and has the following specific advantages that: (1) the test paper is simple to operate, does not need special instruments and equipment, and can be conveniently and directly used for detectionin fields and other fields; (2) the detection time is short (5-8 minutes); the result judgment standard is unified, i.e. for a negative result (-), only one C line appears; and for a positive result(+), T line and C line appear at the same time; and (3) the test paper is verified to have good sensitivity, and the lowest detection limit can reach 0.1microg / mL.

The invention discloses a microfluidic protein chip for high-through detecting genetically modified crops. The microfluidic protein chip comprises a nucleic acid chip which is synthesized on the basis of mu ParafloTM microfluidic protein chip and contains six nucleotide sequences and 6 types of antibody-oligonucleotide composite probes, thereby simultaneously detecting 6 transgenetic proteins of trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1Ac), trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1c), trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1F), trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1Ab), soybean trypsin (SBTI) and neomycin phosphate transferase II gene (NPTII). The invention further provides a transgenic assay kit which comprises a mu ParafloTM microfluidic deoxyribose nucleic acid (DNA) chip which contains six types of probes, six types of antibody-oligonucleotide composite probes, six types of detection antibodies and the like.

This invention is directed to characterizing a host system suitable for the production of functional transgenic proteins, such as anti-human IgG, for use in applications requiring government regulatory approval. It is well known that regulatory agencies required stable, consistent master cell banks and master cell lines for the production of transgenic proteins in order to ensure sufficient material for appropriate characterization, clinical trials, and potential sales. Current plant production systems require the establishment of seed banks for this purpose. However, there are many draw backs related to such a system for the production of a continuous reliable transgenic protein source. An aspect of this invention is directed to characterizing a plant production system suitable for transgenic proteins that meet the stringent regulatory requirements. Another aspect of this invention exemplifies the production and characterization of an anti-human IgG for use as a blood grouping reagents, through the expression of corresponding genes in transgenic alfalfa plants. The cDNAs of the heavy and light chains of a human IgG-specific IgG2a(kappa) murine mAb (C5-1) were transferred into alfalfa through Agrobacterium infection. Transgenic plants expressing the light- and heavy-chain encoding mRNAs were obtained and plants from the Fl progeny (obtained by sexual crossing) were found to express fully assembled C5-1. Furthermore, the transgenic protein was stable in vivo, as well as during extraction and purification procedures. Purification yielded a unique H2L2 form with a reactivity indistinguishable from hybridoma-derived C5-1 in standardized serological tests. Results indicate that plant-derived transgenic proteins, such as mAbs can be used as diagnostic reagents as effectively as hybridoma-derived mAbs, and demonstrates the usefulness of the transformed alfalfa system to produce large amounts of proteins, including multimeric proteins such as mAbs.

The invention discloses a method to produce transgene albumen medicine that takes galactophore expression using adenovirus as carrier. The feature is that: using copying defect type adenovirus framework plamid vector to carry different target albumen gene sequence, and gaining reconstructed albumen carrier from packaging cell in E1A sequence containing Ad5 adenovirus gene group, and gaining instantaneous high expression external target gene medicine albumen. The operation process includes the following steps: separating and cloning target medicine albumen gene sequence; constructing confluence eukaryon expression carrier to testing target albumen to gain external expression right medicine albumen gene; rebuilding the target albumen gene with the adenovirus gene group frame molecule to gain the adenovirus carrier of rebuilt target medicine albumen gene; inducing into animal galactophore; excreting the needed target medicine albumen from latex. The rebuilt albumen could be directly usedto develop kinds of health products and medicine.

The invention relates to a transgenic protein detection method combining antibody modification and terahertz spectrum. The invention aims to provide the method with the characteristics of high accuracy and rapid and convenient detection. According to a technical scheme involved in the invention, the transgenic protein detection method combining antibody modification and terahertz spectrum comprises the steps of: (1) quartz slide cleaning; (2) quartz slide surface modification; (3) quartz slide surface activation; (4) antibody immobilization; (5) quartz slide surface redundant site close; (6) transgenic protein immobilization; (7) acquisition of the terahertz time-domain spectrums of a to-be-measured point on a quartz slide and a reference point; and (8) comparison of the terahertz time-domain spectrums of the to-be-measured point on the quartz slide and the reference point.