Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Transgenic protein detection method combining antibody modification and terahertz spectrum

A technology for antibody modification and protein detection, applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems of difficulty in meeting the needs of rapid detection, time-consuming, etc., and achieve high accuracy and time-saving effects

Active Publication Date: 2014-01-22
ZHEJIANG UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for detecting genetically modified products, such as polymerase chain reaction, enzyme-linked immunoassay, etc., but these methods are relatively time-consuming and difficult to meet the growing demand for rapid detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Transgenic protein detection method combining antibody modification and terahertz spectrum
  • Transgenic protein detection method combining antibody modification and terahertz spectrum
  • Transgenic protein detection method combining antibody modification and terahertz spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] Terahertz wave is an electromagnetic wave with a wavelength between microwave and infrared radiation, and its frequency is 0.1-10THz. Its photon energy is very low, only one millionth of that of X-rays, and the photon energy of terahertz waves is lower than that of various chemical bonds, so it will not cause ionization reactions harmful to human body or detection materials. In general, the vibrational absorption frequency of chemical bonds in organic molecules is mainly manifested in the ordinary infrared band. However, for relatively weak interactions between molecules (such as hydrogen bonds, etc.) Hertz infrared band. Therefore, although the energy of terahertz radiation is very low, a large number of molecules, especially many organic macromolecules (DNA, protein, etc.), show strong absorption and dispersion in this frequency band. Terahertz time domain spectroscopy (THz-TDS) is a new research and detection technology developed internationally in recent years. S...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a transgenic protein detection method combining antibody modification and terahertz spectrum. The invention aims to provide the method with the characteristics of high accuracy and rapid and convenient detection. According to a technical scheme involved in the invention, the transgenic protein detection method combining antibody modification and terahertz spectrum comprises the steps of: (1) quartz slide cleaning; (2) quartz slide surface modification; (3) quartz slide surface activation; (4) antibody immobilization; (5) quartz slide surface redundant site close; (6) transgenic protein immobilization; (7) acquisition of the terahertz time-domain spectrums of a to-be-measured point on a quartz slide and a reference point; and (8) comparison of the terahertz time-domain spectrums of the to-be-measured point on the quartz slide and the reference point.

Description

technical field [0001] The invention relates to a method for detecting a transgenic protein, in particular to a method for detecting a transgenic protein in combination with antibody modification and terahertz spectroscopy. Background technique [0002] With the development of biotechnology and the widespread application of transgenic technology, transgenic products have gradually entered people's lives. Due to the lack of understanding of the new combinations and traits of genetically modified organisms and the expression of traits in different genetic backgrounds, as well as the impact on the environment and humans, it is very necessary to do a good job of safety testing and evaluation of genetically modified organisms. At present, there are many methods for detecting genetically modified products, such as polymerase chain reaction, enzyme-linked immunoassay, etc., but these methods are relatively time-consuming and difficult to meet the growing demand for rapid detection....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/3586
Inventor 谢丽娟徐文道刘湘江
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com