The invention relates to the technical field of
molecular biology detection, in particular to a
TaqMan-MGB fluorescent quantitative
polymerase chain reaction (PCR) detection specific primer (as shown in SEQ ID No. 1 and SEQ ID No. 2), a
TaqMan-MGB fluorescent quantitative PCR detection probe as shown in SEQ ID No. 3) and a
TaqMan-MGB fluorescent quantitative PCR detection method for
seneca valley virus (SVV). The method comprises the steps of drawing a
standard curve; extracting ribonucleic acid (
RNA) of a sample
virus; carrying out reverse transcription on the
RNA of the sample
virus; enabling the product of the reverse transcription to have a TaqMan-MGB fluorescent quantitative PCR, and reading the result. The primer provided by the invention has better specificity and sensitivity; the
MGB probe provided by the invention is shorter and is beneficial to probe design, and the Tm value difference between a paired template and a non-paired template is improved, so that the experimental result is more stable and accurate; the method provided by the invention has the advantages of being simple and rapid, easy to operate, visual in results, high in sensitivity, good in stability, real-time quantitative, and the like, and shortens the reaction time.