Immunochromatography test paper assembled by taking chitin/chitosan fiber membrane as colloid label pad and preparation method thereof

An immunochromatography, chitosan fiber technology, applied in the field of biological detection and biomaterials, can solve the problem of no special emphasis, and achieve the effects of improving stability, excellent detection performance, and improving test stability and sensitivity

Active Publication Date: 2017-11-24
RES INST OF ZHEJIANG UNIV TAIZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the currently disclosed prior art, the commonly used materials for this component are glass fiber membranes, filter paper or polypropylene fiber non-woven fabrics. Although they can meet the general needs of use, there is no special emphasis on the excellent properties of the above materials.

Method used

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  • Immunochromatography test paper assembled by taking chitin/chitosan fiber membrane as colloid label pad and preparation method thereof
  • Immunochromatography test paper assembled by taking chitin/chitosan fiber membrane as colloid label pad and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Pregnancy test paper using chitin / chitosan fiber film colloidal gold pad

[0030] 1. Select chitin / chitosan raw materials with 30% deacylation degree;

[0031] 2. Using mechanical disc grinding method, water, 5% sodium hydroxide by mass fraction, and 5% sodium carbonate by mass fraction are used as auxiliary materials to prepare the chitin / chitosan mixture into a wet slurry; After the slurry is taken from the slurry tank, the film material with a thickness of 2 mm is obtained through the electric heating drum, the film winding drum and the film receiving drum, and is washed and dried for later use;

[0032] 3. Prepare colloidal gold-labeled human chorionic gonadotropin (HCG) mouse antibody 1 solution.

[0033] 4. Spray the solution evenly on the chitin / chitosan film at a ratio of 1 ml to 15 square centimeters, and dry it for 2 hours in an environment with a humidity of 25% and a temperature of 30 degrees Celsius to make a colloid release pad.

[0034] 5. In ...

Embodiment 2

[0036] Example 2 Methamphetamine (Ice Poison) Competitive Inhibition Method Detection Test Paper Using Chitin / Chitosan Fiber Membrane Colloidal Gold Pad

[0037] 1. Select pure chitosan raw materials;

[0038] 2. Dissolve chitosan raw materials in acetic acid, and prepare chitosan fibers through wet spinning process, and obtain dry film material with a thickness of 1.5 mm through general non-woven fabric weaving process for future use.

[0039] 3. Prepare the methamphetamine monoclonal antibody-colloidal gold solution.

[0040] 4. Soak the chitosan film material in the antibody-colloidal gold solution, take it out, and dry it for 6 hours in an environment with a humidity of 35% and a temperature of 20 degrees Celsius to make a colloidal release pad.

[0041] 5. In an environment with a humidity of 35% and a temperature of 20 degrees Celsius, the nitrocellulose coated with T-line (coated with methamphetamine-bovine serum albumin) and C-line (coated with goat anti-mouse IgG pol...

Embodiment 3

[0043] Example 3 Soybean transgenic Bt-Cry1Ab / Ac detection test paper using chitin / chitosan fiber film colloidal carbon pad

[0044] 1. Select chitin / chitosan raw materials with 5% deacylation degree;

[0045] 2. Chitin / chitosan raw materials were dissolved in ionic solvent LiCl / DMAc to make a solution, and the solution was cast on a polytetrafluoroethylene template. After the solvent was volatilized, it was heated and dried to obtain a chitin / shell with a thickness of 2.5mm. The polysaccharide in-situ membrane material is ready for use;

[0046] 3. Prepare colloidal carbon-labeled Bt-Cry1Ab / Ac monoclonal antibody solution.

[0047] 4. Spray the solution evenly on the chitin / chitosan film at a ratio of 1 ml to 16 square centimeters, and dry it for 5 hours in an environment with a humidity of 20% and a temperature of 27 degrees Celsius to make a colloid release pad.

[0048] 5. Cut a chitin / chitosan membrane of appropriate size as a sample pad for later use.

[0049] 6. In a...

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Abstract

The invention provides immunochromatography test paper assembled by taking a chitin / chitosan fiber membrane as a colloid label pad and a preparation method thereof. The test paper has the advantages of uniform adsorption of negative-charge colloid particles, hydrophilic steady flow, neutral biomacromolecules, stable test, high sensitivity, long useful life and the like. In the colloid label pad, pure chitin fiber and deacylated chitosan fiber are mixed in any ratio, and a fiber membrane with thickness of 0.2-4mm is prepared by a pulping method, spinning method or solution method; the test paper which can load multiple kinds of colloid particle markers and are assembled with different detection membranes can be widely applied to the fields such as physical health, disease detection, drug and doping detection and transgenic protein detection.

Description

technical field [0001] The invention belongs to the field of biological detection and biomaterials, in particular to an immunochromatographic test paper using chitin / chitosan fiber membrane as a colloid carrier. Background technique [0002] Immunochromatography (immunochromatography) is a rapid diagnostic technique that has emerged and is widely used in recent years. Its principle is to fix specific antibodies on a certain zone of the nitrocellulose membrane first, and when one end of the dry nitrocellulose is immersed in the sample ( That is, the solution that may contain the target detection substance), the sample will move forward along the membrane due to capillary action, and when it moves to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. Immunocolloidal gold or immunoenzyme-linked staining can make this area show a certain color, so as to realize specific immunodiagnosis. Immunochromatograph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/558
CPCG01N33/531G01N33/558
Inventor 徐沁黄然
Owner RES INST OF ZHEJIANG UNIV TAIZHOU
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