A method for detection of transgenic proteins based on antibody modification and terahertz spectroscopy

A technology for antibody modification and protein detection, which is applied in measurement devices, material analysis through optical means, instruments, etc., can solve the problems of time-consuming and difficult to meet the needs of rapid detection, and achieve the effect of saving time and high accuracy

Active Publication Date: 2015-08-12
ZHEJIANG UNIV
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Problems solved by technology

At present, there are many methods for detecting genetically modified products, such as polymerase chain reaction, enzyme-linked immunoassay, etc., but these methods are relatively time-consuming and difficult to meet the growing demand for rapid detection

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  • A method for detection of transgenic proteins based on antibody modification and terahertz spectroscopy
  • A method for detection of transgenic proteins based on antibody modification and terahertz spectroscopy
  • A method for detection of transgenic proteins based on antibody modification and terahertz spectroscopy

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Embodiment Construction

[0032] Terahertz wave is an electromagnetic wave with a wavelength between microwave and infrared radiation, and its frequency is 0.1-10THz. Its photon energy is very low, only one millionth of that of X-rays, and the photon energy of terahertz waves is lower than that of various chemical bonds, so it will not cause ionization reactions harmful to human body or detection materials. In general, the vibrational absorption frequency of chemical bonds in organic molecules is mainly manifested in the ordinary infrared band. However, for relatively weak interactions between molecules (such as hydrogen bonds, etc.) Hertz infrared band. Therefore, although the energy of terahertz radiation is very low, a large number of molecules, especially many organic macromolecules (DNA, protein, etc.), show strong absorption and dispersion in this frequency band. Terahertz time domain spectroscopy (THz-TDS) is a new research and detection technology developed internationally in recent years. S...

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Abstract

The invention relates to a transgenic protein detection method combining antibody modification and terahertz spectrum. The invention aims to provide the method with the characteristics of high accuracy and rapid and convenient detection. According to a technical scheme involved in the invention, the transgenic protein detection method combining antibody modification and terahertz spectrum comprises the steps of: (1) quartz slide cleaning; (2) quartz slide surface modification; (3) quartz slide surface activation; (4) antibody immobilization; (5) quartz slide surface redundant site close; (6) transgenic protein immobilization; (7) acquisition of the terahertz time-domain spectrums of a to-be-measured point on a quartz slide and a reference point; and (8) comparison of the terahertz time-domain spectrums of the to-be-measured point on the quartz slide and the reference point.

Description

technical field [0001] The invention relates to a method for detecting a transgenic protein, in particular to a method for detecting a transgenic protein in combination with antibody modification and terahertz spectroscopy. Background technique [0002] With the development of biotechnology and the widespread application of transgenic technology, transgenic products have gradually entered people's lives. Due to the lack of understanding of the new combinations and traits of genetically modified organisms and the expression of traits in different genetic backgrounds, as well as the impact on the environment and humans, it is very necessary to do a good job of safety testing and evaluation of genetically modified organisms. At present, there are many methods for detecting genetically modified products, such as polymerase chain reaction, enzyme-linked immunoassay, etc., but these methods are relatively time-consuming and difficult to meet the growing demand for rapid detection....

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/3586
Inventor 谢丽娟徐文道刘湘江
Owner ZHEJIANG UNIV
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