Auxiliary method for detecting whether genetically modified protein exists in food

An auxiliary detection and protein technology, which is used in material inspection products, measuring devices, biological tests, etc., can solve the problems of unreliable sample results and large differences in results, and achieve reliable and reliable test results, simple and easy to operate.

Inactive Publication Date: 2017-01-11
黎志春
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Application number: 201510005351.6 The device disclosed in the Chinese patent document, although it can be used for the rapid detection of protein in food, but there are large differences in the results of multiple parallel experiments of one sample, and the results of individual samples are not reliable

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  • Auxiliary method for detecting whether genetically modified protein exists in food
  • Auxiliary method for detecting whether genetically modified protein exists in food

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Embodiment Construction

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0034] Nuclei isolation buffer: 0.25M sucrose, 18mM PIPES (pH6.8), 5mM MgCl 2 , 60mM KCl, 15mM NaCl, 1mM CaCl 2 , 1.0% Triton X-100, 1 mM PMSF (protease inhibitors are added now). Prepare freshly and store at 4°C.

[0035] Nuclei lysis buffer: 50 mM HEPES (pH7.5), 150 mM NaCl, 1 mM EDTA, 1.0% SDS, 0.1% sodium choloxycholate, 1.1% Triton X-100, 1 μg / mL pestatin A and 1 μg / mL aprotinin (Protease inhibitors are added now). Prepare freshly and store at 4°C.

[0036] TBST buffer: 12.114g Tris base, adjust pH to 7.5, 16.332gNaCl, 1M1Tween-20, add water to 1L.

[0037] DAB Horseradish Peroxidase Chromogenic Kit was purchased from Biyuntian, the product number is P0202.

[0038] The invention provides a method for auxiliary detection of whether there are genetically modified in...

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Abstract

The invention discloses an auxiliary method for detecting whether genetically modified protein exists in food. The method comprises steps as follows: total protein is extracted; the total protein is injected to sample adding holes of a detection device, holes in a first perforated plate are closed, the detection device comprises the first perforated plate, a sample adding device and a hollow fiber polypropylene film tube, the sample adding device comprises a second perforated plate, a cone and multiple sample adding channels, the hollow fiber polypropylene film tube is arranged between the first perforated plate and the second perforated plate, and a first antibody specifically bound with to-be-detected genetically modified protein is attached to the inner wall; the holes in the first perforated plate are opened, and washing is performed; the holes in the first perforated plate are closed again, and a second antibody is injected into the sample adding holes in the detection device; the holes in the first perforated plate are opened again, and washing is performed; whether a mark of the second antibody exists in a hollow fiber polypropylene film is detected, and if the mark exists, the genetically modified protein exists in the total protein and existence of a genetically modified ingredient in the raw material is determined.

Description

technical field [0001] The invention relates to a method for assisting in detecting whether there is transgenic protein in food. Background technique [0002] One or several exogenous genes are transferred to a specific organism through genetic engineering technology, and the corresponding products (polypeptides or proteins) are effectively expressed. This process is called transgenesis. Foods processed from GMOs are genetically modified foods. According to the different sources of genetically modified food, it can be divided into plant genetically modified food, animal genetically modified food and microbial genetically modified food. Genetically modified food has many advantages: it can increase crop yield; it can reduce production cost; it can enhance the ability of crops to resist insect pests and viruses; it can improve the storability of agricultural products. The safety evaluation of GMOs is the prerequisite for the marketization and commercialization of GMOs and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/545
CPCG01N33/68G01N33/545
Inventor 黎志春
Owner 黎志春
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