The invention belongs to the technical field of apple
seedling propagation, and discloses a rapid propagation and
sugar-free rooting culture method and application of apple
rootstock malus prunifolia.The rapid propagation and
sugar-free rooting culture method comprises the following steps of collecting
malus prunifolia branches, carrying out water culture for about 15 days, taking grown tender shoots as explant materials, carrying out disinfection, inoculating into a
primary culture medium, and culturing in an illumination environment; after
seedling division, inoculating the seedlings into asubculture medium, and continuously culturing in an illumination environment; after growing for 30-40 days, selecting
malus prunifolia seedlings which grow robustly, transferring the malus prunifoliaseedlings into a
sugar-free culture box, placing the sugar-free culture box on a special culture shelf for sugar-free culture, introducing
carbon dioxide gas, and carrying out sugar-free rooting culture for 25-35 days in an illumination environment to emerge seedlings. The culture medium is not prone to
pollution, under the condition that
gas exchange with the outside is guaranteed,
photosynthesis of plants is fully utilized, the rooting rate and the
transplanting survival rate of malus prunifolia
tissue culture seedlings are increased, rooting and
seedling hardening are combined, the
time cost is saved, and high-quality and efficient production of the malus prunifolia
tissue culture seedlings can be accelerated.