131 results about "Endocrine disruptor" patented technology

The invention belongs to the field of food inspection, relates to an assay determination method for endocrine disruptors in food, and particularly relates to a method for analyzing and determining a plurality of endocrine disruptors in milk powder and liquid milk. The method comprises the steps of dissolving a sample with water, adding an organic solvent mixable with water, extracting a to-be-detected material by ultrasonic, adding a sodium salt to make the organic solvent separated from a water phase to realize liquid-liquid extraction, taking an organic solvent containing quantitative to-be-detected material, purifying by extraction with a solid phase filled with C 18 materials, deriving by dansyl chloride, and analyzing and detecting four types of 26 endocrine disruptors in the food by using ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry at the same time. The method can overcome the disadvantages that a conventional technology cannot realize simultaneous analysis of the plurality of the endocrine disruptors in the food by once chromatographic sample injection, can increase sensitivity of the quadrupole-time of the flight-mass spectrometry, and realize accurate analysis of the endocrine disruptors in the food by using the quadrupole-time of the flight-mass spectrometry.

A polycarbonate resin reduced in the release of environmental hormones which comprises structural units represented by general formula (A) and structural units represented by general formula (B), the structural units represented by general formula (A) accounting for 5 to 90 mol % of all the structural units, and which has an intrinsic viscosity of 0.30 to 0.60 dl / g. The polycarbonate resin is produced through the copolymerization of a bispenol whose ability to link to estrogen acceptors is low with an aliphatic dihydroxy compound. The resin hence has the basic properties inherent in polycarbonates, i.e. mechanical strength and heat resistance, and is reduced in the release of environmental hormones.

The invention relates to a preparation method of magnetic mesoporous molecular imprinted hybrid silicon spheres. The preparation method comprises the following steps: firstly preparing Fe3O4 magnetic microspheres by adopting a solvothermal method, then coating mesoporous silica on the surfaces of the Fe3O4 magnetic microspheres through a sol-gel method and finally synthesizing the magnetic mesoporous molecular imprinted hybrid silicon spheres on the surfaces of the magnetic mesoporous silicon spheres by utilizing an RAFT (reversible addition-fragmentation chain transfer) molecular imprinting technology. The prepared magnetic mesoporous molecular imprinted hybrid silicon spheres can be used for selectively removing alkylphenol in drinking water. The preparation method disclosed by the invention has the advantages that the preparation method of the magnetic mesoporous molecular imprinted hybrid silicon spheres is reasonable in process and easy to implement; when the materials are used for removing alkylphenol type endocrine disruptors, the materials have good chemical stability and reusability and are easy to realize active adsorption, identification and separation against the alkylphenol; and according to the preparation method, the properties of magnetic mesoporous microspheres and a molecular imprinted polymer can be combined into a whole, the using efficiency of the molecular imprinting technology can be greatly improved and the range of molecular imprinting applications can be further expanded.

The invention relates to a preparation method of a magnetic functionalized graphene oxide based molecularly imprinted hybrid material. The preparation method comprises the following steps: firstly, loading Fe3O4 magnetic nanoparticles on the surface of graphene oxide by chloroformylation reaction; and synthesizing the magnetic functionalized graphene oxide based molecularly imprinted hybrid material on the surface of the magnetic functionalized graphene oxide serving as a carrier by utilizing a molecular imprinting technology. The prepared magnetic functionalized graphene oxide based molecularly imprinted hybrid material can be used for selectively removing estrogen in drinking water. The preparation method of the magnetic functionalized graphene oxide based molecularly imprinted hybrid material has the advantages that the process is reasonable and easy to implement; when being used for removing endocrine disruptors, the material has good chemical stability and reusability, and active adsorption, identification and separation of the estrogen are easily carried out; and the preparation method has strong magnetic response, preordainment, identity and practicality, and can greatly improve the using efficiency of the molecular imprinting technology and expand the range of molecular imprinting applications.

The invention discloses a titanium-dioxide charcoal composite material and a preparation method thereof, and belongs to the field of semiconductor photocatalysts. According to the titanium-dioxide charcoal composite material, the microstructure of charcoal keeps the structure of wood tissue, titanium dioxide is uniformly dispersed on charcoal surface, and titanium dioxide has anatase phase and rutile phase at the same time. The method comprises: firstly performing pretreatment on a wood biological template; dipping the wood biological template in a precursor solution, using a sol-gel method or a hydro-thermal method to depositing TiO2 particles in micropores of the wood template, so as to obtain the wood biological template of titanium dioxide; and finally sintering in a muffle furnace to obtain the titanium-dioxide charcoal composite photocatalyst. The prepared titanium-dioxide charcoal composite material is applicable to remove environmental endocrine disruptors in water and other organic pollutants in water, has the adsorption removal rate of 32.3-86.5%, the photocatalysis removal rate of 11-20% and the total adsorption / photocatalysis synergic removal rate of 50.3-97.5%, and has relatively good application prospect.

In the method for efficiently extracting endocrine disruptors in a sample of the present invention, the sample to be tested is preliminarily treated by filtering, grinding, homogenizing, ultrasonication, protein precipitation, protein hydrolysis or degreasing to make a sample solution of an aqueous matrix; adjust the pH value of the sample solution, Or add inorganic salts or organic salts to increase the ionic strength of the sample solution to reduce the distribution coefficient of endocrine disruptors in the water phase; use the membrane disc solid phase extraction device to make the sample solution flow continuously or "flow-stop-flow" intermittent Through the activated nylon nanofiber membrane, the endocrine disruptors in the sample are retained by the nylon nanofiber to achieve extraction. The invention synergizes the advantages of solid-phase membrane extraction and nano-adsorption media, and can realize efficient extraction of internally distributed interfering substances in various actual samples with only a few milligrams of nylon nanofiber membrane and hundreds of microliters of elution solvent , The extraction and enrichment process of separation, adsorption, concentration and elution can be completed within a few minutes to ten minutes.

The invention provides a preparation method and an application method of a dummy template molecularly imprinted polymer selectively enriching bisphenol endocrine disruptors. The preparation method comprises the following steps: dissolving dummy template molecules, a crosslinking agent, a functional monomer and an initiator in a pore forming solvent to prepare a pre-polymerization solution, carrying out a polymerization reaction at 50-70DEG C for 24-48h; and grinding a white block polymer generated through the polymerization reaction, sieving, settling to obtain polymer particles with the particle size of 45-63[mu]m, carrying out soxhlet extraction or ultrasonic extraction with methanol / acetic acid and methanol as an extraction solvent, and removing residual template molecules and unreacted substances in order to obtain the dummy template molecularly imprinted polymer. The invention also provides a solid phase extraction filler. The filler is the dummy template molecularly imprinted polymer. The dummy template molecularly imprinted polymer has very strong specific selectivity and enrichment ability to bisphenol F, bisphenol E, bisphenol A, bisphenol B, bisphenol AF, tetrabromobisphenol A and other bisphenol compounds, solves the template bleeding problem during trace analysis, and realizes the rapid, senseitive, accurate and efficient detection of the bisphenol endocrine disruptors in environment, foods and biological samples.

A nonionic surfactant which is directly produced by causing an aliphatic alcohol (a1) to add an alkylene oxide (b1), is represented by the general formula R<1>O-[(C2H4O)m / (AO)n]-(C2H4O)p-H, and has a value of Mw / Mn in a specific range, and in which the distribution constant c determined using the following equation derived from the Weibull distribution law is 1.0 or smaller. c=(v+n0 / n00-1) / [Ln(n00 / n0)+n0 / n00-1] (4) (In the equation (4), v is the average of (m+n+p) in the above general formula; n00 is the number of moles of the aliphatic alcohol (a1) used in the reaction; and n0 is the number of moles of the aliphatic alcohol (a1) remaining unreacted.) Due to the specific composition and the specific molecular weight, the surfactant is comparable in surface activity to nonionic surfactants of the alkylphenol type and has no fear of generating an endocrine disruptor. A surfactant obtained by making the nonionic surfactant anionic is improved in foaming property and reduced in odor and skin irritation and hence gives an excellent detergent composition.

The invention relates to a chlorophenol compound measuring method, specifically to a method for determining a trace chlorophenol endocrine disruptor in water. The method comprises the following steps of: adding NaH2PO4 phase separation salt, adding a Na2HPO4-H3PO4 buffer solution to adjust pH value of the solution, adding a [Bmim]BF4 hydrophilic ionic liquid for extraction separation, separating by a centrifuge, taking an ionic liquid phase, and finally determining the content of the chlorophenol endocrine disruptor through HPLC. According to the invention, the ionic liquid is used as an extractant, NaH2PO4 is used as the phase separation salt, and the trace chlorophenol endocrine disruptor in water is determined by the utilization of the ionic liquid aqueous biphasic extraction-high performance liquid chromatography (HPLC) method, which has advantages of short phase separation time, no easy emulsification during the extraction process, nontoxicity, no pollution and the like in comparison with polymer aqueous biphasic extraction. The method provided by the invention has high accuracy and good precision.

The invention discloses a chitosan composite gel water purifying agent, which is gel prepared from solution of chitosan, cellulose, silica gel and activated carbon according to the mass ratio of 1:1-2.5:2-3.5:3-5. The invention also discloses a method for preparing the chitosan composite gel water purifying agent, which comprises the following steps of: adding the cellulose, the silica gel and the activated carbon into the solution of chitosan in turn; fully stirring at a high speed until black and homogeneous semifluid gel is formed, and obtaining the chitosan composite gel water purifying agent. The water purifying agent is natural and nontoxic, has functions of high-efficiency adsorption and flocculation, can be used for deep purification of an underwater environment polluted by endocrine disruptors and also can be used for emergency treatment in public health water pollution emergency. Meanwhile, the preparation method makes operation simple, reduces cost and is environmentally-friendly and suitable for industrial production.

Modified activated carbon for removing organic pollutants is prepared from the following raw materials: by weight, 80-90 parts of activated carbon, 6-8 parts of chitosan, 3-4 parts of Arabic gum, 1-2 parts of sodium alginate, 30-34 parts of sepiolite, 12-14 parts of zeolite, 8-10 parts of modified attapulgite and a proper amount of 20-25% hydrochloric acid and water. The modified activated carbon prepared by the method is used as an adsorbent, at the same time, the sepiolite, the zeolite and other substances with strong adsorption ability are added into the modified activated carbon, and the modified activated carbon can be used for removing the organic pollutants in a water treatment process, has the advantages of high purification capacity and long service life, and especially can solve the problem that water treatment methods in the prior art have poor operability on water polluted by endocrine disruptors of high concentration of phenols.

An object of the present invention is to provide a recording material or a recording sheet using, as a color-developing agent, a non-phenol compound that is a safe compound in no danger of corresponding to an endocrine disruptor and is good in color developing performance. The non-phenol compound used in the present invention is at least one selected from the group consisting of compounds represented by the following formulas (I) to (III).

The invention belongs to the technical field of a preparation and application of a primordial water hyacinth biomass carbon material with a porous structure, and specifically discloses a preparation method of a novel electrode material and an application of the electrode material as a cathode in an electro-Fenton system to degrade endocrine disruptors. The electrode material takes water hyacinth biomass powder as a precursor, zinc chloride powder is added in different proportions to perform activating, the novel biomass carbon material is prepared by high temperature calcination under the protection of an inert gas, and finally a porous electrode is obtained as the cathode material for E-Fenton to degrade endocrine disruptors. The material has relatively good electrical conductivity and relatively large specific surface area. The material can be used as a novel electrode material, and a good degradation effect is especially shown in the electro-Fenton experiment.

Disclosed herein is a method for producing a transparent or opaque flooring sheet or a flooring using a styrene-butadiene copolymer resin (as a thermoplastic elastomer) by calendering. The flooring causes no problems associated with environmental hormones, and generates no halogen gases or endocrine disruptors, such as dioxin, during incineration. Further, the flooring can be regenerated and reused at any time, and exhibits superior stability upon installation and mounting and excellent elastic restoration after application of stress due to inherent flexibility of rubber.

Provided herein is a method of effectively quantifying a target material by performing both colorimetry and fluorescence analysis on the same sample, based on metal nanoparticles and an aptamer.

The invention discloses a method for removing endocrine disruptors in water by using iron and manganese double-phase-doped graphene to activate a single persulfate and relates to a method for removing the endocrine disruptors in the water. The invention aims at solving the problems of low removal efficiency and high cost of the existing method for removing the endocrine disruptors in the water. The method comprises the following steps: 1) mixing the single persulfate with the water after pretreatment; 2) regulating a reaction pH value; 3) preparing iron and manganese double-phase-doped graphene; 4) putting iron and manganese double-phase-doped graphene; and 5) separating iron and manganese double-phase-doped graphene by adopting an external magnetic field, thereby completing the method for removing the endocrine disruptors in the water by using iron and manganese double-phase-doped graphene to activate the single persulfate. By using the method disclosed by the invention, the removal rate in removal of the endocrine disruptors in the water can achieve 85%-96%. By using the method disclosed by the invention, the endocrine disruptors in the water can be removed.

The invention relates to a method for quickly detecting environmental endocrine disruptors (EED) in seawater. The method comprises the following steps of: (1) after a seawater sample is subjected to ion concentration regulation, pH value regulation and water bath ultrasound-assisted extraction, enriching a target compound by adopting a solid-phase microextraction device, and then carrying out headspace derivatization; and (2) after thermal desorption, quantitatively analyzing the EED by using a gas chromatograph mass spectrometer (GCMS). The invention effectively solves the problems of large sampling quantity, long sampling period, difficult sample storage and complicated pretreatment in chemical analysis for testing while analyzing the EED in marine environment investigation, obtains a satisfactory result of the analysis of a selected target compound, has the advantages of small organic solvent dosage, high selectivity to an object to be tested, high extraction speed, high sample recovery rate, and the like, and is suitable for analyzing EEDs with lower contents and complicated structures in the marine environment.

The invention relates to a method for constructing a heart cell system of blotchy rockcod, which comprises: taking a heart tissue of the blotchy rockcod as a material, adopting a trypsin digestion method to start primary culture, and culturing the heart tissue in a DMEM / F12 liquid medium which contains fetal calf serum, basic fibroblast growth factors, I-type insulin-like growth factors, chondroitin sulfate and culture supernatant of fin cells of the blotchy rockcod in the log phase and has a pH value of between 7.0 and 7.4; and adopting the trypsin digestion method for subculturing. The heart cell system of the blotchy rockcod constructed by the method is subcultured for 70 generations currently. The technology is scientific and reasonable, is hopeful to be applied to separation and breeding of fish viroids, preparation of virus vaccines and research in the aspects of interaction of viruses and host cells, and so on, can also be taken as a model for researching environmental pollutants in environmental toxicology, and performs pollution monitoring and safety evaluation on various environmental pollutants in the sea such as genic toxins, mutagen, carcinogens, environmental hormones and endocrine disruptors.

The invention belongs to the technical field of analysis chemistry and particularly relates to a method for detecting and analyzing multiple EDCs (endocrine disruptor chemicals) in the environments and food. According to the detection and analysis method, 4'-phosgene-rhodamine is taken as a derivatization reagent, and the multiple EDCs are analyzed through ultrasonic-assisting-in-situ derivatization-dispersive liquid-liquid microextraction in combination with ultra-high performance liquid chromatography triple quadrupole mass spectrometry detection. According to the method, 4'-phosgene-rhodamine containing an intramolecular permanent positive charge is taken as the derivatization reagent for the first time, 23 EDCs are measured, and quantitation is performed with a tandem mass spectrometry multi-reaction monitoring mode in combination with an internal standard method. The method is environment-friendly, simple, rapid, high in sensitivity and good in selectivity, the recovery rate is good, and matrix interference of actual samples is effectively reduced. The method is applicable to detection and analysis of the EDCs in various environments and food samples, and an accurate and reliable technical means is provided for monitoring and evaluation of the environments and the food.

The present invention relates to an isolation material for an endocrine disrupter, which is prepared by immobilizing an anti-endocrine disrupter antibody to a graft-chain-containing carrier made of a polymeric material via covalent binding. According to the present invention, an isolation material for an endocrine disrupter, which is capable of specific and efficient isolation of an endocrine disruptor, and an endocrine disrupter concentration and a clean-up method capable of specific and efficient concentration or clean-up of an endocrine disruptor at a high rate can be afforded.

The present invention relates to a recording fabric comprising a substrate layer, and an ink receiving layer formed on one or both surfaces of the substrate layer and containing glycol-modified polyethylene terephthalate, and a manufacturing method of the recording fabric. A recording fabric according to the present invention does not require an additional printing layer and is excellent in chemical properties, mechanical properties and flatness, thereby preventing a spreading or breaking phenomenon of the recording fabric from occurring when using the recording fabric. Furthermore, a recording fabric according to the present invention is characterized in that the recording fabric does not emit endocrine disruptor and harmful gases during use, incineration or fire.

A mayonnaise-like food, being an edible emulsified matter prepared by mixing and emulsifying soybean milk or bean curd, vinegar, seasoning, spice, emulsifier, and vegetable oil, in which the soybean milk is a concentrated soybean milk at Brix concentration of 20 to 40, or the bean curd is a concentrated bean curd prepared by coagulating a concentrated soybean milk at Brix concentration of 15 or more by use of a coagulant. Since concentrated soybean milk or concentrated bean curd is used, peculiar greenstuff smell and acridity of soybean can be suppressed, and a mayonnaise-like food having rich flavor and smooth texture can be obtained.

The invention discloses an endocrine disrupter high-throughput screening model and screening method, and relates to the field of virtual screening and activity prediction of endocrine disrupters. Themethod comprises the following steps: extracting a first-stage warning structure, a second-stage warning structure and a third-stage warning structure of a compound aiming at a nuclear receptor, forming a nuclear receptor high-throughput screening model by the first-stage warning structure, the second-stage warning structure and the third-stage warning structure; carrying out graded warning structure matching on the target compound through a nuclear receptor high-throughput screening model, and carrying out ligand-ligand matching; Analyzing a receptor binding mode; and semi-quantitatively predicting the binding activity and the interference activity. The method overcomes the defect that the potential nuclear receptor-mediated endocrine disrupter cannot be effectively screened in a high throughput manner in the prior art, can be used for screening the potential nuclear receptor-mediated endocrine disrupter in a high throughput manner, and can be used for judging the receptor competitionactivity and the quasi-antibody activity of the nuclear receptor-mediated endocrine disrupter.

The invention discloses an artificial composite soil layer treatment system capable of reinforcing the removal of endocrine disruptors. The system comprises three layers from top to bottom, namely a degradation layer, an adsorption layer and a filtering and bearing layer in turn, wherein the filling medium of the degradation layer is natural soil, artificially improved soil or the permeable soil obtained by mixing soil and river sand so that the aerobic degradation of endocrine disruptors can be reinforced; the medium of the adsorption layer is a large particle size hard adsorption material with good adsorbability to endocrine disruptors so that endocrine disruptors and other pollutants in the leachate can be further removed through adsorption; and the medium of the filtering and bearing layer is coarse gravels and pebbles which are used to support the upper filling medium and filter fine particle pollutants. The system can be used to remove more than 85% of endocrine disruptors; and the system has higher hydraulic load and lower site requirement and is suitable for the advanced treatment of the slightly polluted water body or the pretreatment process of the groundwater recharge of the regenerated water.

The invention discloses a method for simultaneously measuring various endocrine disruptors in an environment water sample, and belongs to the field of environment detection. The method includes the steps of firstly, taking a water sample, taking supernate after conducting natural depositing or centrifuging, enriching the supernate through an activated solid-phase extraction small column by means of a solid-phase extraction device, and conducting centrifuging or vacuum drying on the extraction column after enriching; secondly, eluting the extraction column through an organic solvent, and conducting volume fixing through methyl alcohol after eluant is concentrated to prepare to-be-measured liquid; thirdly, establishing a quantifying method of target pollutants; fourthly, measuring the concentration of the endocrine disruptors in the water sample through the efficient liquid-phase chromatography-triple quadrupole mass spectrometry. The method is small in reagent consumption, low in cost and few in step, but can simultaneously quantitatively analyze the endocrine disruptors in the water body, several types to more than one dozen of types of traditional concerned endocrine disruptors are expanded into 103 types of endocrine disruptors, the analysis flux is greatly improved, the application range is expanded, and the potential high-risk endocrine disruptors in the environment water sample can be better recognized.

The invention discloses a method for removing endocrine disruptors in water by using cobalt-doped magnetic redox graphene to work in coordination with a persulfate and relates to a method for removing the endocrine disruptors in the water. The invention aims at solving the problems of poor removal effect, high cost and production of toxic byproducts of the existing method for removing the endocrine disruptors in the water. The method comprises the following steps: 1) mixing the persulfate with the water after pretreatment; 2) regulating a reaction pH value; 3) preparing cobalt-doped magnetic redox graphene; 4) putting cobalt-doped magnetic redox graphene; and 5) separating cobalt-doped magnetic redox graphene by adopting an external magnetic field, thereby completing the method for removing the endocrine disruptors in the water by using cobalt-doped magnetic redox graphene to work in coordination with the persulfate. By using the method disclosed by the invention, the removal rate in removal of the endocrine disruptors in the water can achieve 88%-95%. By using the method disclosed by the invention, the endocrine disruptors in the water can be removed.

The invention provides a composite soil layer system for removing typical endocrine disruptors from water. The system comprises a degradation layer located in the topmost layer for enhancing the degradation effect of microorganisms and ensuring the high hydraulic load, wherein volcanic rock is added to filling soil of the degradation layer; an adsorption layer located in the middle for enhancing the adsorption removal effect of a filler for pollutants; and a filtering and supporting layer located at the bottommost layer for supporting and filtering. To-be-treated water is enabled to flow from top to bottom to pass through the degradation layer, the adsorption layer and the filtering and supporting layer sequentially, so that water treatment is realized. The present invention further provides an experimental apparatus for removing typical endocrine disruptors from water. Compared with the prior art, the system is advantaged in that on account of target pollutants, an artificial filter material with high adsorption capability and high biodegradability is adopted to replace a natural soil layer in a traditional soil treatment system to construct a composite soil layer treatment system; and the purpose of water purification is achieved through physical, chemical and biological effects between a medium, which mainly uses the filter material, and the pollutants.

The present invention provides methods for detecting epigenetic, multigenerational DNA alterations caused by toxicants such as endocrine disruptor agents in a subject. The practice of the present invention can be used to diagnose and / or treat a subject having the identified DNA alterations by developing therapeutics, to prevent or delay the onset of associated diseases and / or dysfunctions.

The invention provides a fluorescent material, and also provides a fluorescent molecularly imprinted polymer prepared by the fluorescent material. The fluorescent material is dansyl methacrylic acid, and the preparation method comprises the following steps: performing a sulfonylation reaction of dansyl chloride and aminoethyl methacrylamide hydrochloric acid under the action of a catalyst to obtain a dansyl methacrylic acid solution, performing extraction and purification of the obtained solution to obtain the dansyl chloride fluorescent material of dansyl methacrylic acid. A molecularly imprinted polymer synthesized by using the dansyl methacrylic acid fluorescent material of the invention as a functional monomer not only can be used as a traditional molecular recognition material for specific separation and combination of target template molecules, but also can be used as a sensing material for direct rapid qualitative and quantitative analysis of endocrine disruptors in environment and food.

The invention discloses an immunoaffinity column for simultaneous purification of bisphenol A and its analogues and a preparation method and application thereof. An artificial antigen of bisphenol A and its analogues provided herein is an antigen prepared by coupling bisphenol A shown as any one of formulas I (1-5) and its analogue hapten to a carrier protein; an antibody prepared by immunizationis used to prepare the immunoaffinity column. The immunoaffinity column for simultaneous purification of bisphenol A and its analogues allows various components under test to be highly purified through single extraction; matrix effect of a sample can be substantially completely eliminated; purifying efficiency is greatly improved. The immunoaffinity column can be combined with LC-MS / MS detection technique to estimate exposure of food endocrine disruptors.