Adsorbent for endocrine disruptors and foods and drinks containing the same
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[0124] The sample was prepared by the same method as in (1)(a) above. (b) Prepare a sample of 4-nonylphenol
[0125] 100 mg of 4-nonylphenol (manufactured by GL Science Inc.) was weighed out on an electronic balance with a 10 ml volumetric flask, and dissolved in ethanol to obtain a final volume of exactly 10 ml. Transfer 3ml of this solution with a pipette to another volumetric flask (50ml) and then dilute to 50ml exactly with ethanol.
[0126] The above-mentioned bisphenol B was used as an internal standard. (c) Determination of the absorption of 4-nonylphenol by microorganisms and the residual amount of 4-nonylphenol
[0127] 0.2 ml of the ethanol solution of 4-nonylphenol obtained in (b) was added to a glass test tube containing 3.8 ml of the supernatant of live or heat-treated bacteria as described in (a) above. The mixture was stirred well and then left to stand in a 37°C water bath. After 60 minutes, the cells were pelleted by centrifugation and 0.95 ml of the super...
Embodiment 1
[0172] Statistical analysis was performed on the obtained data according to Dunnett's multivariate control method. Example 1 Lactobacillus casei (Lactobacillus casei) YIT 9029 absorbs bisphenol A (1)
[0173] Live bacteria or heat-treated bacteria of Lactobacillus casei (Lactobacillus casei) YIT 9029 were obtained according to the sample preparation method described in (1) (a) above. Using these samples, the relationship between the concentration of the microorganisms and the amount of bisphenol A was measured. Samples of live or heat-treated bacteria of Lactobacillus casei YIT9029 at different concentrations (0, 0.625, 1.25, 2.5, 5 and 10 g / l) were prepared. 40 µg of bisphenol A was added to these samples and reacted, and then the amount of bisphenol A remaining in the supernatant was analyzed by gas chromatography, thereby determining the amount of absorbed bisphenol A. The result is shown in Figure 1. (result)
[0174] As shown in Figure 1, the amount of bisphenol A ads...
Embodiment 2
[0175] Therefore, it can be determined that foods and beverages prepared using these microorganisms do not necessarily need to contain live bacteria, but that these foods and beverages can be prepared under a wide range of conditions such as temperature, pH, and the like. Example 2 Lactobacillus casei (Lactobacillus casei) YIT 9029 absorbs bisphenol A (2)
[0176] Using living bacteria of the microorganism Lactobacillus casei (Lactobacillus casei) YIT 9029, the relationship between the bacterial concentration, the amount of absorbed bisphenol A, and the amount of residual bisphenol A was investigated. Live bacterial samples of Lactobacillus casei (Lactobacillus casei) YIT 9029 were prepared at different concentrations (0, 0.625, 1.25, 2.5, 5 and 10 g / l). To these samples was added 20 μg of bisphenol A. After the reaction, according to the GC / MS method and the gas chromatography method described in the above-mentioned (1) (c) and (1) (e), and according to the calculation formu...
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