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Preparation of electro-polymerization molecularly imprinted polymer hollow channel paper device and application of paper device in instant pesticide residue detection

A technology of molecular imprinting and polymer, which is applied in the construction of paper analysis devices, can solve the problems of slow liquid flow, inability to move freely, loss of solution volatilization, etc., and achieve the goal of reducing preparation cost, improving repeatability, and reducing detection time Effect

Inactive Publication Date: 2014-12-03
UNIV OF JINAN
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AI Technical Summary

Problems solved by technology

But at present, the microfluidic paper chip laboratory still promotes the liquid flow through the capillary force of the porous paper fiber. Therefore, the flow speed of the liquid is slow and takes a long time. Bacteria and microbeads cannot move freely in paper fibers

Method used

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  • Preparation of electro-polymerization molecularly imprinted polymer hollow channel paper device and application of paper device in instant pesticide residue detection
  • Preparation of electro-polymerization molecularly imprinted polymer hollow channel paper device and application of paper device in instant pesticide residue detection

Examples

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Embodiment

[0035] Example: （ Organophosphates, such as omethoate)

[0036] A method for preparing an electropolymerization molecularly imprinted polymer hollow channel paper device for detecting omethoate, comprising the following steps:

[0037] (1) Use Adobe Illustrator CS4 software on the computer to design the hydrophobic pattern of the top filter paper, including the entrance (diameter 4mm), detection area (diameter 4mm) and exit (diameter 4mm), the distance between the entrance and the detection area is 20mm, and the distance between the entrance and the exit is 30mm. The hydrophobic pattern of the hollow channel layer filter paper includes an inlet (diameter 4mm), a hollow channel (width 2mm) and an outlet (diameter 4mm), and the distance between the inlet and the outlet is 30mm. The hydrophobic pattern of the bottom filter paper includes an inlet (diameter 4mm), a hydrophilic channel (width 2mm) and outlet (diameter 4mm), the distance between inlet and outlet is 30mm;

[0038](...

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Abstract

The invention discloses preparation of an electro-polymerization molecularly imprinted polymer hollow channel paper device and an application of the paper device in instant pesticide residue detection. A wax printing pattern and a hollow channel cutting pattern are designed on a computer by utilizing AdobeIllustratorCS4 software; a hydrophobic area can be printed by a wax printing machine; an entrance, an exit and a hollow channel are cut by a laser cutting machine; a carbon working electrode, a carbon counter electrode and an Ag / AgCl reference electrode are printed on a working area by the screen printing technique; molecularly imprinted polymers are electro-polymerized on the working electrode by a cyclic voltammetry method; a plurality of pieces of prepared filter paper are stacked between glass sheets sequentially to prepare the electro-polymerization molecularly imprinted polymer hollow channel paper device, and the electrode in the device is connected with an electrochemical workstation to realize instant detection of pesticide residues.

Description

technical field [0001] The invention relates to the technical field of rapid, low-cost and high-selection detection of pesticide residues, and more specifically relates to the construction of a paper analysis device suitable for on-site instant detection. Background technique [0002] Acute poisoning incidents caused by food contamination have been reported frequently in recent years, but the physiological changes caused by the chronic harm of pesticides to the human body are often easily overlooked because there are no obvious symptoms. Some pesticide varieties have cumulative toxicity, and even produce three toxicities: carcinogenic, teratogenic, and mutagenic. With the improvement of people's knowledge and living standards, people pay more and more attention to the methods and technologies of pesticide content determination in food. At the same time, as my country's international trade develops more frequently, the world Each country has higher and higher requirements for...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/48
Inventor 葛慎光于京华颜梅黄加栋张彦刘海云王衍虎
Owner UNIV OF JINAN
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