Construction and application of electrochemical sensor based on copper porphyrin base covalence organic frame material

A covalent organic framework, porphyrin-based technology, applied in the field of electrochemical sensors and analysis and detection, can solve the problems of high price, long time, and cumbersome processing.

Active Publication Date: 2019-11-08
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these detection methods have high sensitivity and good selectivity, they are time-consuming, expensive, and sample pretreatment is cumbersome.

Method used

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  • Construction and application of electrochemical sensor based on copper porphyrin base covalence organic frame material
  • Construction and application of electrochemical sensor based on copper porphyrin base covalence organic frame material
  • Construction and application of electrochemical sensor based on copper porphyrin base covalence organic frame material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the construction of electrochemical sensor

[0034] (1) Preparation of carboxylated multi-walled carbon nanotubes (MWCNTs-COOH): 0.5 g of multi-walled carbon nanotubes was weighed and dispersed in 90 mL of concentrated nitric acid, heated to reflux at 80 °C, and stirred for 40 h. After the reaction, cool to room temperature, wash with distilled water until neutral, and vacuum dry at 50° C. to obtain purified multi-walled carbon nanotubes. Disperse 300 mg of purified multi-walled carbon nanotubes in a mixed solution of concentrated sulfuric acid (45 mL) and concentrated nitric acid (135 mL), and stir at 50 °C for 6 h. Repeated washing until neutral, the product is MWCNTs-COOH;

[0035] (2) Synthesis of copper porphyrin-based covalent organic frameworks (CuP-SQ COFs): a mixture of 162 mg metallic copper tetra-p-aminophenylporphyrin and 51 mg squaraine was added to 2 mL of n-butanol and o-dichlorobenzene In a mixed solvent (volume ratio 1:1), ultrasonically...

Embodiment 2

[0039] Embodiment 2, the detection of low concentration guanine and adenine

[0040] (1) Configuration of sample solution: Prepare 15 µmol / L and 25 mL of guanine and adenine solutions in phosphate buffer solution with pH=3.5 respectively;

[0041] (2) Electrochemical detection: the modified electrode MWCNTs-COOH / CuP-SQ COFs / Co NPs / GCE was used as the working electrode, the platinum electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode, and differential pulse voltammetry was used (DPV) Perform electrochemical detection on the test solution of guanine and adenine to obtain the maximum peak current Ipa value, according to the linear relationship Ipa = -0.9967c -0.7715 and Ipa = -0.6047c -0.5828, respectively calculate guanine and adenine concentration of purines. The calculated results are 15.34µmol / L, 14.81µmol / L.

Embodiment 3

[0042] Embodiment 3, the detection of high concentration guanine and adenine

[0043] (1) Configuration of sample solution: prepare 80 µmol / L and 25 mL of guanine and adenine solutions in phosphate buffer solution with pH=3.5 respectively;

[0044] (2) Electrochemical detection: the modified electrode MWCNTs-COOH / CuP-SQ COFs / Co NPs / GCE was used as the working electrode, the platinum electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode, and differential pulse voltammetry was used (DPV) Perform electrochemical detection on the test solution of guanine and adenine to obtain the maximum peak current Ipa value, according to the linear relationship Ipa = -0.2539c -30.1341 and Ipa = -0.1898c -11.0629, respectively calculate guanine and adenine concentration of purines. The calculated results are 80.28 µmol / L, 49.67 µmol / L.

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Abstract

The invention provides preparation of an electrochemical sensor based on a copper porphyrin base covalence organic frame material. Dispensing parts are adopted for modifying a bare electrode through carboxylic carbon nano-tubes, the copper porphyrin base covalence organic frame material is modified through a circular voltmeter-ammeter method, then, a constant potential deposition method is used for depositing metal cobalt nano-particles CoNPs on the surface of the modified electrode, the obtained modified electrode is the electrochemical sensor MWCNTs-COOH / CuP-SQ COFs / CoNPs / GCE based on the copper porphyrin base covalence organic frame material. The electrochemical sensor is adopted as a working electrode, a platinum electrode is adopted as a counter electrode, a saturated calomel electrode is adopted as a reference electrode, and a differential pulse voltmeter-ammeter method is adopted for simultaneously detecting guanine and adenine. The constructed electrochemical sensor has the advantages of being high in sensitivity, low in detection limit, wide in linear range, good in stability, high in interference resistance and the like.

Description

technical field [0001] The invention relates to a construction method of an electrochemical sensor based on a copper porphyrin-based covalent organic framework material, in particular to the simultaneous detection of guanine and adenine by the electrochemical sensor, and belongs to the field of electrochemical sensors and analysis and detection. Background technique [0002] Guanine (2-amino-6-ketopurine) and adenine (6-aminopurine) are two important purine bases in deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Purine bases are important biomolecules that play important roles in genetic information storage, cell proliferation, protein biosynthesis, and regulation of the cardiovascular system. Abnormal changes in the content of purine bases in organisms may affect the immune system and cause various diseases, including cancer, epileptic lupus erythematosus and anemia. Therefore, accurate detection of guanine and adenine in biological samples is of great significanc...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/30G01N27/48
Inventor 郭昊王明玥吴宁刘慧杨武
Owner NORTHWEST NORMAL UNIVERSITY
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