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Double recognition dopamine imprinting electrochemical sensor and preparation method and application thereof

A dopamine and electrochemical technology, which is applied in the field of dual-recognition dopamine imprinted electrochemical sensors and its preparation, can solve the problems of inaccurate imprinting sites, exposed monomer functional groups on the surface of polymer membranes, and inaccurate specific recognition.

Inactive Publication Date: 2014-09-03
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, the detection of dopamine can utilize the borate compound and the molecule of the ortho-dihydroxy structure to be covalently bonded under alkaline conditions to form a five-membered boronic ester complex, and the formed covalent bond is not stable under acidic conditions. It can be detected by the principle of rapid breakage, but this detection method is interfered by other adjacent dihydroxy structure substances (such as epinephrine EP, norepinephrine NE, ascorbic acid AA and uric acid UA, etc.)
Molecularly imprinted electrochemical sensors prepared based on molecularly imprinted technology can also be used to selectively detect dopamine, but this detection method has the defect of imprecise specific recognition of target molecules
The main reason is that the functional monomer in the electrochemical sensor is non-covalently bound to the template molecule, the imprinting site (cavity) is not accurate, and the functional groups of the monomer on the surface of the polymer membrane are exposed more, so that the non-specific adsorption The interference is more obvious, and the imprinting factor is not high

Method used

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  • Double recognition dopamine imprinting electrochemical sensor and preparation method and application thereof
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  • Double recognition dopamine imprinting electrochemical sensor and preparation method and application thereof

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preparation example Construction

[0026] The invention provides a method for preparing a double-recognition dopamine imprinted electrochemical sensor, the method comprising the following steps:

[0027] (1) 3-aminoboronic acid, 2,5-dimethoxyfuran and acetic acid are carried out contact reaction to prepare the operation of the functional monomer of structure as shown in formula (I);

[0028] (2) In the presence of a buffer solution, the functional monomer and dopamine are mixed to form a polymerization solution, and the glassy carbon electrode is placed in the polymerization solution and scanned by cyclic voltammetry to prepare a polymer film modified electrode;

[0029] (3) placing the polymer membrane modified electrode in an acid solution for electrochemical elution to prepare a double recognition dopamine imprinted electrochemical sensor;

[0030]

[0031] In the operation of preparing functional monomer of the present invention, the consumption of 3-aminoboronic acid, 2,5-dimethoxyfuran and acetic acid ...

Embodiment 1

[0046] 1) Preparation of functional monomers

[0047] Dissolve 100 mg (0.646 mmol) of 3-aminophenylboronic acid in 10 mL of acetonitrile, and slowly add 0.167 mL (1.292 mmol) of 2,5-dimethoxytetrahydrofuran and 0.018 mL (0.323 mmol) of acetic acid while stirring. The above mixed solution was heated to reflux at 80° C. for 6 h to obtain a yellow-brown crude solution. After extraction and purification by column chromatography, a dark pure solution was obtained. Transfer to a rotary evaporator and heat and evaporate at 80°C to obtain a light yellow powder solid product, namely Py-PBA.

[0048] The characterization data of Py-PBA are: 1 H NMR (CD 3 OD,300MHz):δ(ppm):6.262(bs,2H,Pyr-H),7.154(s,2H,BOH),7.417-7.465(m,1H,Ar-H),7.634(d,1H,Ar -H), 7.829 (bs,1H,Ar-H).

[0049] 2) Preparation of polymer membrane modified electrode

[0050] After polishing the glassy carbon electrode on the suede, the K 3 [Fe(CN) 6 ] / K 4 [Fe(CN) 6 ] solution (concentration: 0.1 mmol) was treated ...

Embodiment 2

[0054] According to the method of Example 1, the difference is that the values ​​of the pH of the buffer solution in the process of preparing the polymer film modified electrode are respectively 5, 6, 7, 8, and 9 for the prepared electrochemical sensor, and the electrode The chemical sensor is a working electrode, in a 0.05mol / L PBS solution with pH=7.0, detects 1×10 by DPV method -4 The current signal of mol / L dopamine, the scanning potential is -0.2~0.6V. Test results such as image 3 As shown, the electrochemical sensor prepared at pH 8 has the strongest electrochemical sensitivity to DA.

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Abstract

The invention discloses a double recognition dopamine imprinting electrochemical sensor and a preparation method and application thereof. The method comprises the following steps: (1) conducting contact reaction on 3-amino boronic acid, 2,5-dimethoxy furan and acetic acid to prepare functional monomers; (2) in the presence of a buffer solution, mixing the functional monomers and dopamine to form a polymerization liquid, placing a glassy carbon electrode in the polymerization liquid, and conducting scanning preparation of a polymer film modified electrode in the polymerization liquid in accordance with the a cyclic voltammetry; and (3) placing the polymer film modified electrode in an acidic solution for electrochemical elution, so as to prepare the double recognition dopamine imprinting electrochemical sensor. The electrochemical sensor provided by the invention has excellent specificity recognition ability and anti-interference ability for the detection of dopamine.

Description

technical field [0001] The invention relates to the technical fields of electrochemical sensors and molecular imprinting, in particular to a double-recognition dopamine imprinting electrochemical sensor and its preparation method and application. Background technique [0002] In recent years, molecular imprinting technology has developed rapidly, especially molecularly imprinted polymers (MIPs) (referring to polymers with specific selection and recognition for target molecules (also known as template molecules or imprinted molecules)) have a high level in the field of molecular recognition. application value. This technology usually constructs cavities complementary to the molecular size, three-dimensional structure and functional groups of the target molecule inside the polymer, so as to realize the purpose of recognition and separation of the target molecule. Due to the excellent ability of MIPs to selectively recognize molecules, it has gradually replaced enzymes and ant...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 阚显文钟敏庞署芬晏利琴
Owner ANHUI NORMAL UNIV
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