Method for detecting odor substance butanedione based on olfactory receptor sensor

Abstract

The invention discloses a method for detecting an odor substance butanedione based on an olfactory receptor sensor. The method comprises the following steps of: at first, expressing olfactory receptor protein ODR-10 with a His6-tag on an HEK-293 cell surface, extracting and effectively immobilizing the olfactory receptor protein ODR-10 on a quartz crystal microbalance surface through an aptamer so as to form the olfactory receptor sensor; testing a series of odor material butanedione with different set concentrations, thus obtaining a butanedione-resonance frequency variation standard curve; and then, testing a gas which has unknown concentration and needs to be tested, thereby obtaining the concentration of the odor substance-butanedione in the gas to be tested according to the quartz crystal microbalance resonant frequency and the butanedione-resonance frequency variation standard curve. The method is simple in required instrument and convenient to operate, a problem that the olfactory receptor protein ODR-10a of a sensitive element and a quartz crystal microbalance are stably coupled with each other is solved, and the odor substance butanedione is quickly and specially detected.

Description

technical field [0001] The invention relates to a detection technology, in particular to a method for detecting the odor substance diacetyl based on an olfactory receptor sensor. Background technique [0002] The biological olfactory system can quickly and sensitively perceive and distinguish odorants in the air, thanks to the ability of olfactory receptors to specifically bind to odorant molecules in the air. In the design of traditional gas sensors, conductive polymers and polymer materials are mostly used as sensitive materials, and the disadvantage is poor specificity. There are many kinds of odor molecules, most of which are small molecular weight volatile organic gases with different structures. Especially when the air in the detection environment contains multiple odors, it becomes relatively difficult to specifically detect a certain odor. Emerging biosensors intend to solve this problem from the perspective of bionics, imitating the biological olfactory system to u...

AI Technical Summary

Problems solved by technology

In the design of traditional gas sensors, conductive polymers, polymer materials, etc. are mostly used as sensitive materials, and the disadvantage is that the specificity is poor

There are many types of odor molecules, most of which are small molecular weight volatile organic gases with different structures. Especially when the air in the detection environment contains a variety of odors, it becomes relatively difficult to specifically detect a certain odor

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