Method for detecting odor substance butanedione based on olfactory receptor sensor
A technology of olfactory receptors and sensors, applied in the field of detection, can solve the problems of difficult specific detection and poor specificity, and achieve the effects of improving immobilization efficiency and distribution density, improving stability, and improving sensitivity
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[0023] The following describes in detail the basic principles and specific steps of using the olfactory receptor protein ODR-10 as a sensitive element combined with a quartz crystal microbalance sensor to prepare an olfactory receptor sensor for gas detection.
[0024] The present invention detects the method for odor substance diacetyl based on olfactory receptor sensor, comprises the following steps:
[0025] 1. Preparation of olfactory receptor protein ODR-10
[0026] 1.1 Construction of expression vector
[0027] First, the plasmid containing the full-length cDNA of the olfactory receptor protein ODR-10 gene 3 and the rho-tag signal peptide 2 sequence was genetically engineered PEGFP-N1 / rho-tag / odr-10 (The gene sequence of which is shown in SEQ ID NO.1) is properly modified to construct the expression vector of olfactory receptor protein 5 suitable for the use of olfactory receptor sensors, so that the N-terminus has a fusion expression of His 6 -tag tag 6; first, design...
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