Wild type EGFR high expressing recombinant HEK 293 cells
A HEK293-EGFR, wild-type technology, applied in the direction of cells modified by introducing foreign genetic material, recombinant DNA technology, microbial assay/inspection, etc., can solve the application of wild-type full-length EGFR gene cells related biological characteristics research Issues that have not been reported
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[0029] In the present invention, on the basis of constructing eukaryotic expression carrying pcDNA3.1(+)-EGFR of the wild-type EGFR full-length gene, HEK293 cells are transfected to obtain a recombinant HEK293-EGFR cell strain that stably and highly expresses EGFR molecules.
[0030] Compared with the negative control cells not transfected with the expression vector, the EGFR expression of the recombinant HEK293-EGFR cell line increased by 4 to 5 times, which can be used in drug screening targeting EGFR to increase the sensitivity and specificity of drug screening;
[0031] And compare the wild-type EGFR high expression cell HEK293-EGFR and the cancerous EGFR high expression cell line A431, apply to the study of the biological characteristics of EGFR and the difference comparison of EGFR pathway proteins, and improve the effectiveness of discovering functional proteins.
[0032] The following is a detailed description of this aspect:
[0033] The present invention is based on ...
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