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Pinellia detoxification, tissue culture and quick propagation method

A technology of tissue culture rapid propagation and pinellia, applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve the problems of incomplete removal of viruses, large stem tip tissues, and easy pollution. Achieve the effect of solving the problem of pinellia germplasm degradation, easy detoxification, and easy seed retention

Inactive Publication Date: 2006-07-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But the problem that this shoot tip meristem detoxification method exists is: to cut very small shoot tip meristem, need to operate under microscope, has certain difficulty; The cut shoot tip tissue is too large, and the virus cannot be completely removed, and it is easy to cause pollution; therefore, a better method of virus removal is still needed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 (with Pinellia immature seeds as explant)

[0035] 1) Selection and sterilization of explants: In the middle and late May, when the involucral bracts, pericarp and seeds are green, take the immature seeds of Pinellia, soak them in 75% alcohol for 0.5-1.0min, and then use 0.1 % mercuric chloride solution for 20 minutes, and finally rinsed with sterile water for 3 to 5 times, as explants for detoxification tissue culture;

[0036] 2) Basic medium: select MS basic medium, sugar 25g / L, agar 8g / L, pH5.6;

[0037] 3) Induction culture: inoculate the seeds on the induction medium of MS+6-BA 0.5mg / L+NAA 0.5mg / L under sterile conditions, first form callus, and then transplant the callus to MS+ On the differentiation medium of 6-BA 3.0mg / L+NAA0.3mg / L, cluster buds were induced to form rootless tissue cultured seedlings, bulbils and small roots;

[0038] 4) Proliferation culture: Under sterile conditions, inoculate rootless tissue cultured seedlings, bulbils and sma...

Embodiment 2

[0042] Embodiment 2: (with the ripe seed of pinellia radix as explant)

[0043] 1) Selection and sterilization of explants: in late June and early July, when the involucral bracts turn yellow, the pericarp turns white and green, and the seeds are light brown, tea green, and easy to fall off, take the mature seeds of Pinellia ternata through Soak in 75% alcohol for 0.5-1.0 min, then sterilize with 2% sodium hypochlorite aqueous solution for 30 min, and finally rinse with sterile water for 3-5 times, as explants for detoxification tissue culture; 2) basic medium: select MS Basic medium, sugar 20g / L, agar 9g / L, pH5.7;

[0044] 3) Induction culture: inoculate the seeds on the induction medium of MS+6-BA 2.0mg / L+IBA 0.1mg / L under sterile conditions, directly form rootless tissue culture seedlings, and then form bulbils and small roots;

[0045]4) Proliferation culture: Under aseptic conditions, inoculate rootless tissue cultured seedlings, bulbils and small roots on the proliferat...

Embodiment 3

[0049] Embodiment 3: (with the immature seed of pinellia radix as explant)

[0050] 1) Selection and sterilization of explants: In the middle and late May, when the involucral bracts, pericarp and seeds are green, take the immature seeds of Pinellia, soak them in 75% alcohol for 0.5-1.0min, and then use 0.1 % mercuric chloride solution for 20 minutes, and finally rinsed with sterile water for 3 to 5 times, as explants for detoxification tissue culture;

[0051] 2) Basic medium: select MS basic medium, sugar 30g / L, agar 7g / L, pH5.8;

[0052] 3) Induction culture: Inoculate the seeds on the induction medium of MS+6-BA 1.5mg / L+NAA 0.15mg / L under sterile conditions, form callus by induction, and then inoculate the callus on MS+ On the callus differentiation medium of 6-BA2.5mg / L+NAA 0.2mg / L, cluster buds are induced to form rootless tissue cultured plantlets, bulbils and small roots;

[0053] 4) Proliferation culture: Under sterile conditions, inoculate rootless tissue cultured ...

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Abstract

The invention relates to a method of tissue culturing and fast breeding pinellia tuber of toxicity-removing, comprising the following steps: employing the immature seed and / or mature seed of pinellia tuber as explant, breeding large quantity of tissue culturing sprout of toxicity-removing, bulbil of toxicity-removing and small root tuber of toxicity-removing after evoked culturing, enrichment culturing, root growing culturing, replanting and virus detection. The invention is characterized by the simple inoculation, low inoculation pollution rate, high survival rate; simple, fast and through toxicity removing; 5-10 times breeding coefficient, fast breeding, suitable for factory breeding and commercial production.

Description

technical field [0001] The invention relates to a technique for rapid propagation of plant virus-free tissue culture, in particular to a method for rapid propagation of Pinellia pinellia through tissue culture. Background technique [0002] Pinellia is a perennial herbaceous plant of Araceae, most of which are wild. Due to the large demand for medicinal and export, the wild resources are exhausted and it is difficult to meet the demand. Artificial cultivation has now begun. Artificial cultivation generally uses small tubers and bulbils as reproductive organs, but the reproductive coefficient is low, and the annual reproductive coefficient is only 2 to 3 times; at the same time, in cultivation and production, due to long-term asexual reproduction, virus infection and continuous accumulation make its yield and medicinal value Components are all reduced, causing the cultivation and production of pinellia to be greatly restricted. Studies have shown tha...

Claims

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Application Information

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IPC IPC(8): A01C1/00C12N5/04A01H4/00
Inventor 徐刚陈炯汪一婷牟豪杰吕永平陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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