Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris
A technology of sterilization system and cultivation method, which is applied in the field of cultivation of Cordyceps militaris, can solve the problems of inability to increase the biotransformation rate of the medium, low biotransformation rate of medium raw materials, low water content of the medium, etc., so as to improve the biotransformation rate , high practical value and application value, high drying effect
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Embodiment 1
[0063] Embodiment 1: the sterilization system of culture medium
[0064] The sterilization system of the culture medium of the present invention includes: a steam generator 1 connected with an exhaust pipe and a sterilizing cabinet 2 (with an inner tank 2a and an outer tank 2b, and the inner tank and the outer tank are connected with an exhaust pipeline) , the exhaust pipe connected to the end of the sterilization cabinet 2 is divided into two branches, and a straight pipe (such as image 3 In the lower right corner, an exhaust switch is installed), and the other exhaust pipe (with an exhaust switch) is equipped with a vacuum tank 5 connected in sequence (its volume is 5 times the volume of the inner tank 2a of the sterilizer, and the vacuum tank is soaked In the cooling circulation water tank) and the water circulation vacuum pump 3 (equipped with a vacuum gauge), another pipeline is drawn from the exhaust pipeline connected to the inner tank 2a and the outer tank 2b of the s...
Embodiment 2
[0065] Embodiment 2: Utilize the sterilization system of embodiment 1 to sterilize the culture medium
[0066] specific method:
[0067] The method of this embodiment can not only sterilize the culture medium in the cultivation process of Cordyceps militaris, but also sterilize other solid medium, especially the granular material medium, which can not only remove free water, strengthen the drying degree, but also accelerate Material cooling.
[0068] (1) Put the medium into the sterilizer, and supply steam with a pressure of 0.11MPa to the sterilizer from the steam generator, and sterilize at 121°C for 20 minutes;
[0069] (2) Turn on the exhaust switch on the two branch roads to exhaust the steam. When the steam pressure drops to half (0.055MPa), close the exhaust switch on the straight exhaust pipe, and turn on the water circulation vacuum pump to force the exhaust steam;
[0070] (3) When the pressure indication of the water circulation vacuum pump is -0.05MPa, turn off ...
Embodiment 3
[0074] Embodiment 3: the improved culture method of Cordyceps militaris of the present invention
[0075] 1. Experimental time: April 2012
[0076] 2. Experimental materials:
[0077] (1) Strain: the Cordyceps militaris strain with the strain number HX-64 (from the Biology Center of Beijing Academy of Agriculture and Forestry Sciences);
[0078] (2) Strain solid medium: solid PDA slant, ingredients: 300 grams of potatoes, 20 grams of glucose, 20 grams of agar, 1000 ml of water, natural pH.
[0079] (3) Seed medium for liquid shake flasks: 250 grams of peeled potatoes (boiled juice), 8 grams of glucose, 2.5 grams of peptone, 0.6 grams of potassium dihydrogen phosphate, 1.2 grams of magnesium sulfate, 1000 ml of water, and natural pH.
[0080] (4) Nutrient solution for fruiting body production: 2.2% soybean, 1.5% glucose, 4.5% milk powder, 0.8% potassium dihydrogen phosphate, 0.06% magnesium sulfate, vitamin B 115mg / 1000ml, add water to 1000mL to make a nutrient solution , ad...
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