32results about How to "Suitable for factory breeding" patented technology

The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; the inoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.

The invention discloses a greenhouse seedling cultivating method for kalopanax septemlobus seeds.The method comprises steps of seed collecting, seed processing, seed sprouting, sowing, seedling stage management, outplanting and kalopanax septemlobus seedling cultivating.The greenhouse seedling cultivating method is characterized in that for the seed sprouting process, the bottom of a sprouting disc provided with holes at the bottom is loaded with vermiculite which is 5-6 cm deep, the vermiculite is watered with sufficient water, the disinfected seeds are broadcast on the surface of the vermiculite and covered with a layer of vermiculite of 1-1.5 cm, the sprouting disc is covered with a layer of gauze and watered with sufficient water, the sprouting disc is put in a sunlight greenhouse, the greenhouse temperature is controlled to range from 0 DEG C to 25 DEG C under natural lighting, and the sprouting disc containing the seeds is kept wet.According to the greenhouse seedling cultivating method for the kalopanax septemlobus seeds, highly integrated control over the whole seedling cultivation process is achieved, the method is easy to master, the obtained cultivated kalopanax septemlobus seedlings are high in survival rate, and the technology is convenient to popularize and specially suitable for large-scale industrial seedling cultivation.

The invention belongs to the field of agricultural biotechnology and relates to a method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches. The method comprises the steps of prothallium culture process, multiplication culture process, sporophyte induction process, rooting induction process, and transplant and manage process. The technological process is simple; by reproduction in the form of prothallium, a great amount of prothallium can be obtained; by the processes of multiplication, sporophyte induction, sporophyte rooting and transplant, a great number of seedlings capable of being used for production and culture can be obtained; the obtained seedlings are at the same physiological age and grow uniformly; and the method is suitable for industrial seedling raising and scale culture.

The invention discloses a tissue culturing method for regenerating somatic cells of an aquatic iris, belonging to the technical field of plant tissue culturing. The tissue culturing method comprises the steps of: (1) preparing a culturing medium; (2) culturing detoxified tissue culture seedlings of the aquatic iris; (3) transplanting and culturing the tissue culture seedlings; and the like. A tissue culture rapid propagation technical system established by using aseptic seedling leaves as embryoid inducing explants is characterized by high propagation coefficient, high speed, complete structure, high regeneration rate, low production cost and the like; compared with the general organ tissue culturing method, the tissue culturing method for regenerating somatic cells of the aquatic iris has the advantages of shortening the whole culturing period to 75 days, increasing the seedling proliferation coefficient by 2.4 times, reaching a culture seedling rooting rate of 100 percent and a transplanting survival rate of 100 percent, being applicable to industrial seedling culture and realizing commercialized production. The method can be popularized and applied to enterprise production of the aquatic iris.

The invention discloses a high-efficiency expanding propagation method for crossbred Chinese pennisetum, and belongs to the technical field of plant tissue culture. The high-efficiency expanding propagation method comprises the following steps: explant selection and sterilization, callus induction, embryonic callus induction and proliferation, multiple shoot induction, transplanting acclimatization, and the like. A stable and efficient expanding propagation system is established, the callus induction is up to 90%, the expanding propagation coefficient of the embryonic callus induction is as high as 20 times, the multiple shoot induction rate is up to 95%, and the acclimatization survival rate is up to 97%. Due to adoption of an ex vitro rooting mode, the production cost is greatly lowered, the method is applicable to industrialized seedling production, and assurance is provided or commercial planting of high-quality Chinese pennisetum.

The invention discloses a method of a tissue culture of iris ensata. The good effects of quick multiplication rate, stable hereditary feature, high reproduction coefficient and exuberant test-tube plantlet growth are reached mainly through the steps of primary culture, secondary culture, multiplication culture, rooting culture and hardening-plantlet transplantation during a tissue culture process, a germinating rate of a callus of the iris ensata is up to 96%, the multiplication multiple is up to 4.35 times, the rooting rate is up to 100%, and the transplanting survival rate is up to 98%.

The invention relates to the field of biotechnology application and discloses a rapid seedling propagation method for Chinese aralis. The method comprises the following specific steps: 1, washing a top dormant bud of Chinese aralis, which is taken as an explants, by using running water, soaking by using 70 percent alcohol, disinfecting in a 0.1 percent mercury bichloride solution, and finally thoroughly rinsing by using sterile water; 2, cutting the disinfected explant into small blocks of 0.5-1cm, inoculating to a prepared induction medium for cultivation, and differentiating adventitious buds, wherein the culture conditions are as follows: the light intensity is 1500-20001x, the temperature is 25+ / -1 DEG C and the illumination is 12 hours; 3, transferring the induced adventitious buds to a subculture medium for performing enrichment culture, so that bud seedlings are healthy and strong; and 4, inoculating the differentiated adventitious buds into a rooting medium, and transplanting when the adventitious roots grow out, wherein the survival rate of the transplanted seedlings can be over 80 percent. The method disclosed by the invention is easy and feasible to operate, high in propagation speed and suitable for industrialized seedling production.

The invention belongs to the technical field of plant cultivation, and relates to a method for rapidly breeding Spanish moss seedlings through suspension culture. The method comprises the processes of callus induction and propagation, embryogenic callus liquid suspension, embryoid adventitious bud germination, adventitious bud root induction, transplanting and management. The method has simple process flow, and allows a large amount of seedlings used for production and cultivation to be obtained in a short time through tissue culture adopting Spanish moss leaves as a material, the early stage culture period is short, the obtained seedlings have the advantages of consistent physiologic age, consistent growth, small occupied area and low cost, and the method is suitable for factory seedling growth and large scale cultivation, and provides technical support for development of the artificial plantation industry of Spanish moss.

The invention discloses an efficient cultivation method for excellent strains of Danish hibiscus. The method comprises the following steps of material collecting, explant pretreatment, explant adventitious root induction treatment, explant field planting and explant seedling establishment management. The method has the advantages that material collecting is convenient, the materials are rich, and regeneration plants can keep the original excellent character of a maternal plant; operation is easy, the reproduction speed is high, and meanwhile the type and concentration of a rooting promotion solution are strictly optimized and controlled; type and concentration selection of disinfectant, nutritious fluid, nutritious substrates and the like is strictly matched with essential elements needed in the Danish hibiscus plant growing process, and introduction of harmful elements is avoided; explants are placed in a mixed nutritious substrate to directly root in a greenhouse, seedling establishment is achieved through one step, and influences and limits of natural factors such as seasons and territories are avoided; the cost is low, operation is easy, mastering is easy, reproduction expanding can be achieved, and the method is suitable for factory cultivation of the Danish hibiscus.

The invention relates to a seedling-raising fertilizer for tomato, belonging to the technical field of industrial seedling-raising fertilizers. The seedling-raising fertilizer for the tomato is characterized by comprising the following components by weight: 30 to 40% of calcium magnesium nitrate, 35 to 45% of potassium nitrate, 15 to 25% of potassium dihydrogen phosphate, 0.5 to 1% of EDTA-Fe, 0.1 to 0.3% of EDTA-Zn, 0.06 to 0.1% of EDTA-Cu, 0.2 to 0.5% of EDTA-Mn, 0.1 to 0.2% of boric acid, 0.01 to 0.02% of ammonium molybdate and 0.7 to 1.2% of EDTA2Na. In a formula of the fertilizer in the invention, chelated trace elements are used to replace sulfate trace elements, and a complexing agent is added to prevent calcium phosphate from precipitation, so the prepared seedling-raising fertilizer is capable of meeting the nutritional needs of tomato seedling raising and can be completely dissolved; meanwhile, the seedling-raising fertilizer has the advantages of simple components, easily-available raw materials, low cost, convenient operation, economy, practicality, applicability to industrial vegetable seedling raising, etc.

The invention discloses a virus-free tissue culture and rapid propagation method of castor oil plants. The method comprises the following steps: adopting current year's ripe seeds, mixing castor oil plant seeds with coarse sands according to a ratio of 1:5-8, carrying out medium speed grinding and shelling, screening to remove the coarse sands, cleaning the obtained seeds with warm water 2-3 times, immersing the washed seeds in 75% alcohol for 0.5-1.0min, disinfecting the immersed seeds with an aqueous solution of 2% sodium hypochlorite for 20-30min, and flushing the disinfected seeds with sterile water 3-5 times to obtain the flushed seeds used as explants; and carrying out induction culture, propagation culture, rooting culture, transplanting and virus detection to obtain mass propagated virus-free tissue culture seedlings, virus-free plant buds and virus-free small root tubers. Compared with present castor oil plant seedling growing methods, the method disclosed in the invention has the advantages of low inoculation and pollution rate, high transplanting survival rate and high quality.

The invention discloses a cutting fast propagation method of aronia melanocarpa. The method comprises the following steps: selecting an aronia melanocarpa plant to be cultured as a scion mother tree,cutting a strong semi-lignified branch with no diseases or insect pests in a growing period, soaking the lower part of the branch in clear water, placing the branch in a cool environment, and keepingbranches and leaves fresh and alive; acquiring cutting slips by cutting the branch into the cutting slips with a length of 8 centimeters, carrying out disinfection, soaking the lower notches in a rooting agent solution for 2 hours, inserting the cutting slips into a soilless medium subjected to disinfection, maintaining suitable temperature, humidity and illumination conditions, and preventing andtreating diseases and insect pests; and regularly spraying water for moisture preservation, and culturing for 45 days to reach rooting rate of 80% or above, and achieve transplanting conditions, wherein the obtained cutting slips can be used for culturing seedlings for production. The materials in the method provided by the invention are simple and easy to obtain, the operation specifications ofeach link are normative, the culture conditions are controllable, the repeatability is strong, the cost is low, the promotion and the production are facilitated, the rapid propagation of excellent seedlings of aronia melanocarpa is facilitated, and the method has a promotion and application value.

The invention provides a mango seedling grafting method, belongs to the technical field of fruit tree grafting, solves the problems of long seedling period, low survival rate and the like in the prior art, can be used for grafting when the diameter of the base part of a stock is greater than or equal to 5mm, realizes sowing and grafting in the current year and outplanting in the second year, greatly shortens the mango seedling period, and improves the yield. The survival rate is high and can reach 98.4%, and the method is easy to operate and suitable for industrialized seedling culture.

The invention discloses a method of a nanometer tissue culture of iris ensata. The good effects of quick multiplication rate, stable hereditary feature, high reproduction coefficient and exuberant test-tube plantlet growth are reached by mainly adding nanometer additives in primary culture, second culture, multiplication culture and rooting culture during a tissue culture process, a germinating rate of a callus of the iris ensata is up to 98%, the multiplication multiple is up to 4.55 times, the rooting rate is up to 100%, and the transplanting survival rate is up to 98%.

The invention provides a cowberry detoxification breeding method. The cowberry detoxification breeding method comprises the following steps: accelerating germination of cowberry resting shoots, shearing off a bud when the length of the bud reaches 1-3 cm and carrying out sterilizing treatment; cutting off 0.3-0.8 cm of a stem tip from the sterilized bud, and inoculating the stem tip on a foundational medium, and culturing under the conditions that the temperature is 25-28 DEG C, the illumination intensity is 1,800-2,200 Lx, and the illumination time is 12-16 h / d; after the stem tip grows until the length of the stem tip reaches 1.5-2.0 cm, cutting off 0.03-0.05 cm of a stem tip in a sterile environment and inoculating the stem tip to a detoxification medium, carrying out dark culture for 5-9 days at the temperature of 22-25 DEG C, and continuing culturing in an environment in which the temperature is 23-26 DEG C, the illumination intensity is 1,800-2,200 Lx and the illumination time is 12-16 h / d; and inoculating a detoxification seedling to a medium, carrying out rapid multiplication culture, and then carrying out seedling hardening and rooting after the length of the seedling reaches 2.0-3.0 cm.

The invention provides a factory eggplant double-root-cutting grafting seedling raising method. The method comprises the following steps of debugging equipment, preparing raw materials (inoculating rootstocks, scions and the like), carrying out matrix nutrition proportioning, carrying out germination accelerating treatment, sowing and transplanting, carrying out double-root-cutting grafting, healing, carrying out seedling hardening management, and standard finished products emergence. The production design is ordered, the production period is fixed for 80 days, automatic coordination of factory matching water, temperature, fertilizer and air is realized, high grafting survival rate, difficulty in excessive growth, neat and strong seedlings, consistent cultivation standard and strong seedlings are achieved, vegetable seedling quality is improved, soil harmful disease (eggplant verticillium wilt and bacterial wilt) resistance is remarkable, secondary infection prevention effect after plant formation is good, grafting seedling recovery time is shorter and faster, the survival rate is higher, the growth vigor is stronger, the root system is strong, the stress resistance is strong, the yield is high, the quality is better, and the technology breaks through the defects of the conventional grafting technology; and the grafting process is easy to decompose, suitable for industrialized seedling production and capable of reducing labor intensity.

The invention discloses a tissue culturing method for regenerating somatic cells of an aquatic iris, belonging to the technical field of plant tissue culturing. The tissue culturing method comprises the steps of: (1) preparing a culturing medium; (2) culturing detoxified tissue culture seedlings of the aquatic iris; (3) transplanting and culturing the tissue culture seedlings; and the like. A tissue culture rapid propagation technical system established by using aseptic seedling leaves as embryoid inducing explants is characterized by high propagation coefficient, high speed, complete structure, high regeneration rate, low production cost and the like; compared with the general organ tissue culturing method, the tissue culturing method for regenerating somatic cells of the aquatic iris has the advantages of shortening the whole culturing period to 75 days, increasing the seedling proliferation coefficient by 2.4 times, reaching a culture seedling rooting rate of 100 percent and a transplanting survival rate of 100 percent, being applicable to industrial seedling culture and realizing commercialized production. The method can be popularized and applied to enterprise production of the aquatic iris.

The invention relates to a seed treatment method for neat seedling emergence of euscaphis japonica seeds. The seed treatment method comprises the following steps: uniformly mixing harvested, impurity-removed and selected seeds and river sand in the volume ratio of 1 to 3 every year; placing the mixture in a wood box with good drainage; after 300 days, adding 0.5 to 1 weight percent of plant ash, mixing uniformly, and watering often to keep the humidity, wherein the humidity is suitable when the mixture kneaded by a hand can form dough; the seeds start to crack on 350 to 360 days, and the seedscan be screened for sowing when the number of cracks reaches 3 to 5 percent. The germination rate of seed seedlings is over 80 percent; the treatment time is shorter than 360 days; the time from sowing to germinating and growing into bud seedlings is short; the seedlings emerge neatly; the sowing time controllability is high; the euscaphis japonica seed treatment method or a germination accelerating method is suitable for industrialized seedling production; the euscaphis japonica seeds can germinate indoors about three months earlier; the height of the seedlings which are transplanted in a nursery land for planting in current year can reach over 80 cm; bare roots in the current year can be delivered out of the nursery land.

The invention discloses a rheum officinale virus-free tissue culture fast propagation method, and belongs to the technical field of plant virus-free tissue culture fast propagation. According to the preparation method, pure triennial seed plants of rheum officinale are taken as the female parents; strong and large buds are picked from matured mother plants, then soaked in alcohol (75%), and is taken as explants after mercury dichloride disinfection, then the explants are subjected to induction culture, multiplication culture, and rooting culture so as to obtain rooted tissue culture seedlings with a height of 3 to 5 cm, rooted bulbels with a diameter of 0.2 to 0.5 cm, and rooted small earthnuts with a diameter of 0.5 to 1.0 cm, and finally the seedlings, bulbels, and small earthnuts are transplanted to a culture matrix to culture sprouts. The buds are taken as the explants to carry out virus-free culture to obtain seedlings, compared with the conventional bud breeding method, a novel breeding method is provided, the quality of rheum officinale is improved, the breeding speed is accelerated, and the method can be widely popularized and used.

The invention discloses a greenhouse seedling cultivating method for kalopanax septemlobus seeds.The method comprises steps of seed collecting, seed processing, seed sprouting, sowing, seedling stage management, outplanting and kalopanax septemlobus seedling cultivating.The greenhouse seedling cultivating method is characterized in that for the seed sprouting process, the bottom of a sprouting disc provided with holes at the bottom is loaded with vermiculite which is 5-6 cm deep, the vermiculite is watered with sufficient water, the disinfected seeds are broadcast on the surface of the vermiculite and covered with a layer of vermiculite of 1-1.5 cm, the sprouting disc is covered with a layer of gauze and watered with sufficient water, the sprouting disc is put in a sunlight greenhouse, the greenhouse temperature is controlled to range from 0 DEG C to 25 DEG C under natural lighting, and the sprouting disc containing the seeds is kept wet.According to the greenhouse seedling cultivating method for the kalopanax septemlobus seeds, highly integrated control over the whole seedling cultivation process is achieved, the method is easy to master, the obtained cultivated kalopanax septemlobus seedlings are high in survival rate, and the technology is convenient to popularize and specially suitable for large-scale industrial seedling cultivation.

The invention provides a cowberry detoxification breeding method. The cowberry detoxification breeding method comprises the following steps: accelerating germination of cowberry resting shoots, shearing off a bud when the length of the bud reaches 1-3 cm and carrying out sterilizing treatment; cutting off 0.3-0.8 cm of a stem tip from the sterilized bud, and inoculating the stem tip on a foundational medium, and culturing under the conditions that the temperature is 25-28 DEG C, the illumination intensity is 1,800-2,200 Lx, and the illumination time is 12-16 h / d; after the stem tip grows until the length of the stem tip reaches 1.5-2.0 cm, cutting off 0.03-0.05 cm of a stem tip in a sterile environment and inoculating the stem tip to a detoxification medium, carrying out dark culture for 5-9 days at the temperature of 22-25 DEG C, and continuing culturing in an environment in which the temperature is 23-26 DEG C, the illumination intensity is 1,800-2,200 Lx and the illumination time is 12-16 h / d; and inoculating a detoxification seedling to a medium, carrying out rapid multiplication culture, and then carrying out seedling hardening and rooting after the length of the seedling reaches 2.0-3.0 cm.

The invention discloses a method of a tissue culture of iris ensata. The good effects of quick multiplication rate, stable hereditary feature, high reproduction coefficient and exuberant test-tube plantlet growth are reached mainly through the steps of primary culture, secondary culture, multiplication culture, rooting culture and hardening-plantlet transplantation during a tissue culture process, a germinating rate of a callus of the iris ensata is up to 96%, the multiplication multiple is up to 4.35 times, the rooting rate is up to 100%, and the transplanting survival rate is up to 98%.

The invention discloses an efficient cultivation method for excellent strains of Danish hibiscus. The method comprises the following steps of material collecting, explant pretreatment, explant adventitious root induction treatment, explant field planting and explant seedling establishment management. The method has the advantages that material collecting is convenient, the materials are rich, and regeneration plants can keep the original excellent character of a maternal plant; operation is easy, the reproduction speed is high, and meanwhile the type and concentration of a rooting promotion solution are strictly optimized and controlled; type and concentration selection of disinfectant, nutritious fluid, nutritious substrates and the like is strictly matched with essential elements needed in the Danish hibiscus plant growing process, and introduction of harmful elements is avoided; explants are placed in a mixed nutritious substrate to directly root in a greenhouse, seedling establishment is achieved through one step, and influences and limits of natural factors such as seasons and territories are avoided; the cost is low, operation is easy, mastering is easy, reproduction expanding can be achieved, and the method is suitable for factory cultivation of the Danish hibiscus.

The invention discloses a seed tissue culture and propagation method of fourstamen stephania roots. The seed tissue culture and propagation method comprises six steps of seed storage, seed disinfection, initiation culture, induction culture, multiplication culture and rooting culture, and is characterized in that in the induction culture step, an induction culture medium I is used for culturing germinated and inflated seeds to induce aseptic seedlings and an induction culture medium II is used for continually culturing the aseptic seedlings so that the browning rate of the multiple shoots is reduced and the phenomenon that the multiple shoots are partially vitrified is eliminated; and in the multiplication culture step, two steps of multiplication culture are adopted: first step, a lot of multiple shoots can be rapidly cultured; and second step, strong multiple shoot seedlings for rooting and parts of newly-propagated multiple shoots can be cultured, so that the propagation efficiency of the tissue culture method is effectively improved. The method is simple in culture flow, high in propagation coefficient and convenient to operate, is suitable for industrialized seedling production and provides technical supports for industrially culturing fourstamen stephania root seedlings.

The invention relates to a culture method of hyacinth virus-free tissue culture bulb, which belongs to plant propagation technical field. The method comprises the steps of the preparation of a culture medium, the culture of the virus-free tissue culture bulb, the detection of a virus ELISA and so on. The invention can detect tissue culture bulb seedlings rapidly with high sensitivity and can stopthe virus from carrying and transmission. Comparing with the bulbs which are not virus-free, the virus-free tissue culture bulb has rapid growth speed, early flowering stage and bright flower colour.Virus-free tissue culture bulb breeds rapidly, the breeding ratio per month of which can reach 3-5 times more than the bulbs which are not virus-free. The invention is suitable for industrial seedling production and can be widely used in the purification, the rejuvenescence and the development of the hyacinth.

The invention belongs to the technical field of forest biology, particularly relates to a method for in-vitro rapid propagation of lycium ruthenicum by taking young seedlings as explant donors. The method is completed through obtaining of a sterile seedling, inoculation of an explant, subculture multiplication and acclimatization and transplanting. The method comprises the following steps: taking seedling leaves as explants to carry out inoculation, wherein 1 month after the inoculation, the bud forming rate of the explants is 87.5%; taking stems as explants to carry out inoculation, wherein 1 month after the inoculation, the bud forming rate of the explants is 100%; carrying out starvation and drying treatment, carrying out inoculation, wherein 1 month after the starvation and drying treatment, the vitrification relieving rate is 85.3%. The method is extremely high in regeneration efficiency, along with the increase of the reproduction times, the regeneration efficiency increases exponentially, and both the rooting percentage and the transplanting survival rate are above 80%. According to the method, mature seeds are used as the initial experiment materials, so that the storage is convenient and is not limited by the seasons; by combining a vitrification relieving method, the method is particular suitable for the protection, development and utilization of rare lycium ruthenicum resources; the individual reproduction cost is extremely low, so that the method is suitable for the industrialized seedling production of the lycium ruthenicum.

The invention discloses a high-efficiency expanding propagation method for crossbred Chinese pennisetum, and belongs to the technical field of plant tissue culture. The high-efficiency expanding propagation method comprises the following steps: explant selection and sterilization, callus induction, embryonic callus induction and proliferation, multiple shoot induction, transplanting acclimatization, and the like. A stable and efficient expanding propagation system is established, the callus induction is up to 90%, the expanding propagation coefficient of the embryonic callus induction is as high as 20 times, the multiple shoot induction rate is up to 95%, and the acclimatization survival rate is up to 97%. Due to adoption of an ex vitro rooting mode, the production cost is greatly lowered, the method is applicable to industrialized seedling production, and assurance is provided or commercial planting of high-quality Chinese pennisetum.

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; theinoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.