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Virus-free tissue culture and rapid propagation method of castor oil plants

A technique for tissue culture and rapid propagation of castor oil, applied in horticultural methods, botanical equipment and methods, gardening and other directions, can solve problems such as restricting medicinal use and oil output, affecting the application quality of castor oil, virus infection, etc. The effect of incomplete detoxification, solving the degradation of castor germplasm, and restoring yield and quality

Inactive Publication Date: 2016-07-13
岳兰兰
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AI Technical Summary

Problems solved by technology

[0004] At present, castor planting mainly adopts direct seeding in the field, the emergence rate is low, and the virus infection is serious, which greatly limits the output of medicinal and oil, and affects the application quality of castor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Selection and sterilization of explants: select the mature seeds that were born in the current year, mix them with castor seeds: coarse sand at a ratio of 1:5-8, grind at a medium speed to remove the shell, sieve the coarse sand, and wash with warm water 2 to 3 times, soaked in 75% alcohol for 0.5 to 1.0 min, then sterilized with 2% sodium hypochlorite aqueous solution for 20 to 30 min, and finally rinsed with sterile water for 3 to 5 times, as explants for detoxification tissue culture ;

[0030] 2) Basic medium: select MS basic medium, sucrose 25g / L, carrageenan 6.5g / L, pH6.5;

[0031] 3) Induction culture: under aseptic conditions, the seeds were inoculated on the induction medium of MS+6-BA1.2mg / L+NAA0.35mg / L+chlorella powder 0.8mg / L, and the callus was first formed. Then transplant the callus to the differentiation medium of MS+6-BA2.5mg / L+NAA0.2mg / L+chlorella powder 1.2mg / L to induce cluster buds to form rootless tissue cultured plantlets and bulbils;

[0032...

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Abstract

The invention discloses a virus-free tissue culture and rapid propagation method of castor oil plants. The method comprises the following steps: adopting current year's ripe seeds, mixing castor oil plant seeds with coarse sands according to a ratio of 1:5-8, carrying out medium speed grinding and shelling, screening to remove the coarse sands, cleaning the obtained seeds with warm water 2-3 times, immersing the washed seeds in 75% alcohol for 0.5-1.0min, disinfecting the immersed seeds with an aqueous solution of 2% sodium hypochlorite for 20-30min, and flushing the disinfected seeds with sterile water 3-5 times to obtain the flushed seeds used as explants; and carrying out induction culture, propagation culture, rooting culture, transplanting and virus detection to obtain mass propagated virus-free tissue culture seedlings, virus-free plant buds and virus-free small root tubers. Compared with present castor oil plant seedling growing methods, the method disclosed in the invention has the advantages of low inoculation and pollution rate, high transplanting survival rate and high quality.

Description

technical field [0001] The present invention relates to plant detoxification tissue culture rapid propagation technology, in particular to a method of castor plant detoxification tissue culture rapid propagation Background technique [0002] Castor is an annual or perennial herbaceous plant of the genus Castor in the family Euphorbiaceae. At present, castor is cultivated for extracting medicine and industrial oil, or as a landscape tree for viewing its leaves. Among them, the medicinal value is mainly reflected in ricin, which has the effects of detoxification, anti-cancer, swelling and stagnation; castor oil is used in fine chemicals, and can be used to make surfactants, fatty acid glycerides, fatty diols, dry oils, etc. Oil, sebacic acid, stabilizers and plasticizers for polymerization, foam plastics and elastic rubber, etc. Castor oil has high viscosity, high specific gravity (0.958-0.968), does not solidify at a low temperature of -18°C, does not deteriorate or burn at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 岳兰兰
Owner 岳兰兰
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