Rheum officinale virus-free tissue culture fast propagation method

Abstract

The invention discloses a rheum officinale virus-free tissue culture fast propagation method, and belongs to the technical field of plant virus-free tissue culture fast propagation. According to the preparation method, pure triennial seed plants of rheum officinale are taken as the female parents; strong and large buds are picked from matured mother plants, then soaked in alcohol (75%), and is taken as explants after mercury dichloride disinfection, then the explants are subjected to induction culture, multiplication culture, and rooting culture so as to obtain rooted tissue culture seedlings with a height of 3 to 5 cm, rooted bulbels with a diameter of 0.2 to 0.5 cm, and rooted small earthnuts with a diameter of 0.5 to 1.0 cm, and finally the seedlings, bulbels, and small earthnuts are transplanted to a culture matrix to culture sprouts. The buds are taken as the explants to carry out virus-free culture to obtain seedlings, compared with the conventional bud breeding method, a novel breeding method is provided, the quality of rheum officinale is improved, the breeding speed is accelerated, and the method can be widely popularized and used.

Description

technical field [0001] The invention belongs to the technical field of plant detoxification tissue culture and rapid propagation, in particular to a method for rhubarb detoxification tissue culture and rapid propagation. Background technique [0002] Rhubarb, also known as General, is a perennial herb of the Polygonaceae Rhubarb genus. It is mostly born on the edge of mountain forests or on grass slopes. There are two methods of propagation: wild and cultivated. The rhizome of rhubarb is strong, and it has the functions of purging and attacking accumulation, purging fire and detoxification, promoting blood circulation and removing blood stasis, clearing damp and heat, etc. It is a traditional Chinese medicinal material in my country and one of the main export medicinal materials in China. Rhubarb likes cold and cool climates, is cold-resistant, and avoids high temperatures. It grows wild in high mountainous areas with an altitude of about 2000m in the northwest and southwes...

AI Technical Summary

Problems solved by technology

It can be seen that the seed propagation in the existing rhubarb planting method is a traditional method, which is affected by the climate, and, due to the rough sowing method, the emergence rate is low and the growth of the seedlings is poor, which affects the quality of medicinal materials, especially the content of medicinal ingredients. lower

The seed bud propagation is to pick the seed buds and bury them directly in the planting hole to take root. The soil pathogens are complex, and the seed buds have poor disease resistance, which leads to serious contamination of the seed buds, which not only affects the rooting and emergence rate, but also reduces the content of medicinal ingredients. The quality of medicinal materials is low and cannot meet market requirements