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A device and method for annual propagation of apple cotton aphid

A technology of apple cotton aphid and apple, which is applied in the field of apple cotton aphid inoculation, can solve the problems of reducing the success rate of inoculation, easily damaged stylets, and low success rate of inoculation, and achieve the effect of improving the success rate of inoculation and speeding up the progress of experiments

Active Publication Date: 2022-05-10
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time, the success rate of inoculation is low, and the seedlings can only be transplanted in March-April every year, which seriously limits the reproduction of the apple aphid. In the past, the apple aphid was inoculated, and the brush was mainly used to pick the apple aphid for inoculation. On the tender shoots of apple seedlings, it is very easy to damage the stylet and reduce the success rate of inoculation

Method used

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  • A device and method for annual propagation of apple cotton aphid
  • A device and method for annual propagation of apple cotton aphid
  • A device and method for annual propagation of apple cotton aphid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: Make the device of annual propagation apple cotton aphid

[0057] ① Production of plastic cultivation cups: Use an electric soldering iron to solder 4 round holes with a diameter of 50mm on the bottom of a disposable plastic cup with an upper mouth diameter of 8cm, a lower bottom diameter of 5.6cm, and a cup height of 10cm;

[0058] ②Manufacturing of the inoculation rack: use a saw blade to drill a long and narrow groove with a length of 5 cm and a width of 3.5 mm in the middle of the board I with a thickness of 10 cm × 10 cm and a thickness of 3 mm as the groove I. The distance from the upper side is 4.5cm; use a saw blade to saw three small rectangular grooves with an interval of 1cm, a length of 1cm and a width of 3mm on the right side of the rectangular plank II with a thickness of 10cm×5cm and a thickness of 3mm as the groove II , the upper side of the uppermost groove II is about 3cm away from the upper side of the board II; insert the board II into ...

Embodiment 2

[0059] Embodiment 2: A kind of device of annual propagation apple cotton aphid

[0060] The described device for propagating cotton aphids every year is made through the method of Example 1, and includes an inoculation frame and a cultivation plastic cup 1, and the inoculation frame includes wooden boards I 2 and wooden boards II 4 arranged crosswise:

[0061] The cultivation plastic cup is a disposable plastic cup, and its specifications are: the diameter of the upper opening of the cup is 8cm, the diameter of the lower bottom is 5.6cm, the cup height is 10cm, and the volume is 340mL; the bottom of the disposable plastic cup is provided with 4 round holes , the diameter of the round hole is 50mm;

[0062] The plank 1 has a strip-shaped groove I3 drilled in the central position, and the long side of the groove 1 is parallel to the upper side / lower side of the plank 1; the plank 1 has a specification of: 10cm long × width 10cm, thickness 3mm; the specification of the groove I ...

Embodiment 3

[0065] Embodiment 3: Utilize the device of embodiment 2 to carry out annual propagation apple cotton aphid

[0066] ①Cultivation of apple seedlings:

[0067] Cut a healthy apple with a knife, take out the mature and plump seeds, wash off the pulp on the surface of the seeds, add pure water to the plastic cup and soak the seeds in it for about 7 hours; then use pointed tweezers to remove the seed coat and inner Peel off the seed coat, leaving only the embryo and cotyledon; spread a layer of soaked absorbent cotton on the bottom of a disposable plastic culture dish with a diameter of 20 cm, evenly spread the seed embryo and cotyledon on the absorbent cotton, and then cover the seed with a layer of soaked The absorbent cotton is placed in an incubator with a temperature of 25°C, a photoperiod of 12L: 12D, and a humidity of 65 ± 5% to germinate. Use a watering can to spray water every day to keep the absorbent cotton moist.

[0068] ② Apple seedling transplanting:

[0069] Mix t...

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Abstract

The invention discloses a device and method for annual propagation of cotton aphid apple. The device is an insect rack, and an angle is formed by intersecting wooden boards I and II, and a cultivation plastic cup is placed on the angle; the cotton aphid is parasitized Apple seedlings and healthy apple seedlings were transplanted into cultivation plastic cups respectively, and the height and angle of the inoculation device were adjusted according to the height of healthy apple seedlings and apple seedlings parasitized with apple aphid, so as to ensure that healthy apple seedlings could reach The apple seedlings parasitic with the apple aphid are then completed the work of inoculation. The invention can realize the annual planting and propagation of apple seedlings, thereby realizing the annual propagation of the apple cotton aphid without being limited by time, and overcomes the method that only 3-4 months can be transplanted to obtain apple seedlings in production; The damage to aphids caused by inoculation with a writing brush greatly improved the success rate of inoculation. At the same time, the apple cotton aphid was easy to colonize on young apple seedlings, and the standardized test insects were bred to speed up the progress of the experiment.

Description

technical field [0001] The invention relates to an inoculation device and method for the cotton aphid, in particular to a device and a method for the annual propagation of the cotton aphid, which are suitable for the perennial propagation of the cotton aphid and belong to the technical field of cotton aphid inoculation. Background technique [0002] The apple aphid Eriosoma lanigerum (Hausmann) is an insect of the genus Eriosoma lanigerum (Hausmann) of Hemiptera, which is an important quarantine pest at home and abroad. The apple aphid mainly sucks and damages the young shoots of the twigs of apple plants, forming tumors at the damaged cortex, affecting the yield and quality of apples, and causing the death of apple trees in severe cases. [0003] In order to clarify the damage mechanism and prevention and control technology of the apple cotton aphid, the laboratory needs to develop a consistent source of insects for physiological and biochemical, molecular biology, drug scr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01G17/00A01G7/06
CPCA01K67/033A01G17/005A01G7/06
Inventor 周洪旭郭怡王凌云李慧芳滕子文范银君谭秀梅黄飞鹏万方浩
Owner QINGDAO AGRI UNIV
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