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Construction method of microbial electrochemical system for hydrogen phosphide generation reinforcement

A technology of microbial electrochemistry and construction method, applied in the field of microbial electrochemistry, to achieve the effects of convenient operation, shortened start-up time, and convenient material collection

Inactive Publication Date: 2016-03-30
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the currently known phosphine-producing bacteria, there are only solvent-fermenting bacteria: Clostridium sporogenes, Clostridium cochliarium and Clostridium acetobutyricum, which have strong electrochemical activity. may be reinforced

Method used

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  • Construction method of microbial electrochemical system for hydrogen phosphide generation reinforcement

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Enrichment culture of phosphine-producing microorganisms outside the microbial electrolytic cell: 300 g of pig manure, 300 g of anaerobic sludge and 1 L of medium were added to a 3L brown anaerobic bottle. The medium used methanol as a carbon source and COD as 800mg / L, dipotassium hydrogen phosphate as phosphorus source, TP is 50mg / L; ammonium chloride is used as nitrogen source, TN is 30mg / L. Trace element nutrient solution, the addition amount is 1ml / L. The culture medium was ventilated with argon gas for 5 minutes to remove oxygen before use, and cultured anaerobically at 35°C in an incubator, with pH 6.5 and ORP in the range of 380-400mv. Each cycle is 4 days, and the enrichment of phosphine-producing microorganisms is completed after 8 cycles to obtain a domesticated activated sludge mixture rich in phosphine-producing microorganisms.

[0034] (2) Take 10mL of the acclimated activated sludge mixture, centrifuge at 3000r / min for 10min in a centrifuge, wash with...

Embodiment 2

[0037] (1) Enrichment culture of phosphine-producing microorganisms outside the microbial electrolytic cell: 300 g of pig manure, 300 g of anaerobic sludge and 1 L of medium were added to a 3L brown anaerobic bottle. The medium used methanol as a carbon source and COD as 800mg / L, dipotassium hydrogen phosphate as phosphorus source, TP is 50mg / L; ammonium chloride is used as nitrogen source, TN is 30mg / L. Trace element nutrient solution, the addition amount is 1ml / L. The culture medium was ventilated with argon gas for 5 minutes to remove oxygen before use, and cultured anaerobically at 35°C in an incubator, with pH 6.5 and ORP in the range of 380-400mv. Each cycle is 4 days, and the enrichment of phosphine-producing microorganisms is completed after 8 cycles to obtain a domesticated activated sludge mixture rich in phosphine-producing microorganisms.

[0038] (2) Take 10mL of the acclimated activated sludge mixture, centrifuge at 3000r / min for 10min in a centrifuge, wash with...

Embodiment 3

[0041](1) Enrichment culture of phosphine-producing microorganisms outside the microbial electrolytic cell: 300 g of pig manure, 300 g of anaerobic sludge and 1 L of medium were added to a 3L brown anaerobic bottle. The medium used methanol as a carbon source and COD as 800mg / L, dipotassium hydrogen phosphate as phosphorus source, TP is 50mg / L; ammonium chloride is used as nitrogen source, TN is 30mg / L. Trace element nutrient solution, the addition amount is 1ml / L. The culture medium was ventilated with argon gas for 5 minutes to remove oxygen before use, and cultured anaerobically at 35°C in an incubator, with pH 6.5 and ORP in the range of 380-400mv. Each cycle is 4 days, and the enrichment of phosphine-producing microorganisms is completed after 8 cycles to obtain a domesticated activated sludge mixture rich in phosphine-producing microorganisms.

[0042] (2) Take 10mL of the acclimated activated sludge mixture, centrifuge at 3000r / min for 10min in a centrifuge, wash with ...

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Abstract

The invention belongs to the technical field of microbial electrochemistry and discloses a construction method of a microbial electrochemical system for hydrogen phosphide generation reinforcement. The construction method comprises mixing animal wastes and anaerobic activated sludge, adding the mixture into an anaerobic reactor, carrying out culture through a medium to obtain a phosphine-producing microorganism-rich domesticated activated sludge mixed solution, taking the domesticated activated sludge mixed solution, carrying out centrifugation separation, carrying out washing through a phosphate buffer solution, adding inoculation sludge into a microbial electrolysis cell, carrying out biofilm culturing, applying power for the microbial electrolysis cell, adding the electrolysis product into a medium and carrying out anaerobic cultivation for hydrogen phosphide generation reinforcement. Through construction of the microbial electrolysis cell and domestication of microbes, a hydrogen phosphide yield of phosphate reduction and a hydrogen phosphide production rate are improved. The construction method has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial electrochemistry, and in particular relates to a method for constructing a microbial electrochemical system capable of strengthening phosphine production. Background technique [0002] As an important phosphorus carrier in the phosphorus cycle, phosphine is gradually attracting people's attention. A large number of scholars have found that microorganisms can convert phosphorus into phosphine in an anaerobic environment. There are relevant reports in the anaerobic sludge of sewage plants, livestock and poultry manure, wetlands, rice fields and other environments. However, in the research history of nearly a century, the generation mechanism of phosphine has not yet been clearly explained. The content of phosphine produced by microbial reduction is also only in trace level. [0003] The microbial electrochemical system introduces microorganisms on the basis of the electrochemical system. Accor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P9/00
CPCC12P9/00
Inventor 牛晓君刘炜
Owner SOUTH CHINA UNIV OF TECH
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