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Method for breeding cordyceps militaris by utilizing silkworm larvae

A technology of Cordyceps militaris and larvae, applied in botany equipment and methods, applications, gardening, etc., can solve the problems of immature technology of Cordyceps militaris, waste of resources, pollution, etc., and achieve easy scale cultivation, increased success rate, and technological process easy effect

Active Publication Date: 2013-01-23
北京航天农业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are a small number of research reports on the use of silkworm larvae to cultivate Cordyceps militaris, the technology of using silkworm larvae to cultivate Cordyceps militaris is still immature and difficult to promote. The main disadvantages are: the growth period of Cordyceps militaris hosts is 4-5 instars. 6-day-old silkworm larvae, the silk gland protein in silkworm larvae in this growth period is not fully formed, which affects the accumulation of active substances of Cordyceps militaris. Low; in the process of cultivation, cocooning and pupation occur, resulting in waste of resources

Method used

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  • Method for breeding cordyceps militaris by utilizing silkworm larvae
  • Method for breeding cordyceps militaris by utilizing silkworm larvae
  • Method for breeding cordyceps militaris by utilizing silkworm larvae

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Selection of Cordyceps militaris strain host

[0032] Cordyceps militaris is cultivated with cooked silkworms (the 5th instar silkworm larvae develop to the end and stop eating mulberry leaves, which are called cooked silkworms) as hosts. The mature silkworms are required to have transparent bodies and light red feces.

[0033] (2) Preparation of Cordyceps militaris strain

[0034] Add a fruiting body of Cordyceps militaris (frozen preservation or fresh fruiting body) in a good growth state to the sterile PDA slant medium, cultivate it in a dark environment at 19°C, and carry out light culture after the aerial hyphae cover the entire slope , after the aerial mycelium turns yellow completely, gently scrape out the mycelium in a sterile environment, add sterile water and grind to make a suspension of Cordyceps militaris, the concentration of the suspension of Cordyceps militaris is 0.1mg / μL (Based on the mass of fresh mycelium).

[0035] (3) Hypothermic anesthesia a...

Embodiment 2

[0046] (1) Selection of Cordyceps militaris strain host

[0047] Cordyceps militaris is cultivated with cooked silkworms (the 5th instar silkworm larvae develop to the end and stop eating mulberry leaves, which are called cooked silkworms) as hosts. The mature silkworms are required to have transparent bodies and light red feces.

[0048] (2) Preparation of Cordyceps militaris strain

[0049] Add a fruiting body of Cordyceps militaris (frozen preservation or fresh fruiting body) in a good growth state to the sterile PDA slant medium, cultivate it in a dark environment at 20°C, and carry out light culture after the aerial hyphae cover the entire slope , after the aerial mycelium turns yellow completely, gently scrape out the mycelium in a sterile environment, add sterile water and grind to make a suspension of Cordyceps militaris, the concentration of the suspension of Cordyceps militaris is 0.1mg / μL (Based on the mass of fresh mycelium).

[0050] (3) Hypothermic anesthesia a...

Embodiment 3

[0061] (1) Selection of Cordyceps militaris strain host

[0062] Cordyceps militaris is cultivated with cooked silkworms (the 5th instar silkworm larvae develop to the end and stop eating mulberry leaves, which are called cooked silkworms) as hosts. The mature silkworms are required to have transparent bodies and light red feces.

[0063] (2) Preparation of Cordyceps militaris strain

[0064] Add a fruiting body of Cordyceps militaris (frozen preservation or fresh fruiting body) in a good growth state to the sterile PDA slant medium, cultivate it in a dark environment at 20°C, and carry out light culture after the aerial hyphae cover the entire slope , after the aerial mycelium turns yellow completely, gently scrape out the mycelium in a sterile environment, add sterile water and grind to make a suspension of Cordyceps militaris, the concentration of the suspension of Cordyceps militaris is 0.1mg / μL (Based on the mass of fresh mycelium).

[0065] (3) Hypothermic anesthesia a...

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Abstract

The invention relates to a novel method for breeding cordyceps militaris by utilizing silkworm larvae. The method comprises the steps of taking live matured silkworm as the host, vaccinating cordyceps militaris strains through an injection method, and promoting silkworm to sleep, and the vaccination attainment rate is improved; after the method is adopted, the inactivation rate of the silkworm larvae reaches 90% to 100%, and the outgrowth rate of fruiting body reaches 85% to 100%; moreover, the water-solubility protein, polysaccharide, cordycepic acid, cordycepin and adenosine of the cordyceps militaris bred by the method are higher than those of cordyceps militaris bred by silkworm chrysalis in content. The novel method for breeding cordyceps militaris by utilizing silkworm larvae has the advantages of convenience in operation, low breeding cost, good experiment repeatability and wide application prospect, and is suitable for large-scale cultivation.

Description

Technical field: [0001] The invention belongs to the field of edible fungus cultivation, and relates to a method for artificially cultivating Cordyceps militaris, in particular to a method for cultivating Cordyceps militaris with live silkworm larvae as hosts. Background technique: [0002] Cordyceps militaris, also known as Cordyceps militaris or Cordyceps militaris, is a kind of edible and medicinal fungus recognized both at home and abroad. Studies have shown that the types and contents of effective chemical components of Cordyceps militaris are the same or similar to those of Cordyceps sinensis, and have the same or similar functions on the human body. They all contain biologically active substances such as cordycepic acid, cordycepin, polysaccharides, SOD enzymes and ergosterol, It has the effects of improving immunity, anti-tumor, anti-oxidation, anti-fatigue, anti-radiation, lowering blood sugar, lowering blood fat and delaying aging (Wei Huiping, 2004). [0003] Com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 贾俊强桂仲争吴琼英
Owner 北京航天农业生物科技有限公司
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