Method for identifying wheat scab extension resistance through wheat ear top double-flower inoculation
A technology for wheat head blight and wheat ear, which is applied in the field of identification and evaluation of plant disease resistance, can solve problems such as poor consistency of identification results, and achieve the effect of accurately expanding resistance and high success rate
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[0016] The present invention will be further described below. The following examples are only used to illustrate the technical solution of the present invention more clearly, but not to limit the protection scope of the present invention.
[0017] The invention uses the scab-resistant strain R75 and the scab-susceptible strain S98 in the field to carry out dynamic identification of scab inoculation and extended resistance. On the 14th and 21st days after inoculation, the rate of diseased spikelets in the lower part of the inoculated spikelets was dynamically counted, so as to identify the extended resistance of the variety.
[0018] The steps of the method for identifying wheat scab extended resistance of the present invention are: utilize disc sampler on the solid plate culture medium of gibberella strain through activation, take 3-5 pieces of PDA solid medium with gibberella strain and put Put it into 60mL of sterilized mung bean soup medium, then place it on a shaker, and ...
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