67 results about "Gibberella acuminata" patented technology

A substituent thieno [3', 2':5,6] pyrido [4,3-d] pyrimidine-4(3H)-one with bactericiding activity and its preparing process is also disclosed. It can be used as the bactericide with high suppression activity to cotton wilt fungus, the sheath and culm blight bacteria of rice, gray mold of cucumber, etc.

The invention provides a polysubstituted pyrido (4, 3-d) pyrimidine compounds with bactericidal activity and a synthesis method thereof. The structural general formula of polysubstituted pyrido (4, 3-d) pyrimidine compounds of the invention is showed in general formula I which includes three compounds as showed in general formulas I-1, I-2 and I-3; the proposed structure of general formula I-1 isthe development and complement of Patent CN 100335481C while no relevant coverage on the structure of the general formulas I-2 and I-3 is released currently. The experiments indicate that the compounds with the general formula I can obviously inhibit various pathogenic bacteria such as fusarium oxysporum vasinfectum, rhizoctonia solani, botrytis cinera pers, gibberella saubinetii, ring rot of apple, cotton anthracnose, cucumber timberrot, cucumber target leaf spot, scab of cucumber, pepper phytophthora blight, etc., and can be used as the active ingredient of bactericides.

The invention discloses a method for detecting the active thallus amount of Fusarium fujikuroi in gibberellin fermentation broth, and belongs to the technical field of fermentation. The method comprises the steps: (1) the to-be-detected fermentation broth is taken and mixed with a pre-blended XTT-menadione color-producing reagent to form a reaction system, and to-be-detected broth is prepared through incubation; (2) the light density OD450 of the to-be-detected broth at 450 nm is measured through a spectrophotometer; and (3) the measured OD450 value is directly used for representing the activethallus amount of the Fusarium fujikuroi in the to-be-detected fermentation broth, or the dry cell weight in the to-be-detected fermentation broth is calculated according to a formula to represent the active thallus amount of the Fusarium fujikuroi. According to the detecting method, interference of the physical properties of insoluble substances, dead cell residues and filamentous thallus in thegibberellin fermentation broth to detection is greatly reduced, the thallus amount of the Fusarium fujikuroi in the gibberellin fermentation broth can be accurately reflected, operation is easy and convenient and easy to implement, cells do not need to be washed, the detection sensitivity is high, and repeatability is achieved.