67 results about "Gibberella acuminata" patented technology

The invention relates to novel fusarium fujikuroi. A strain is collected in CGMCC (China General Microbiological Culture Collection Center) of China microbe collection management committee on July 15, 2015; the collection number is CGMCC No.11101; the classification name is the fusarium fujikuroi; and the Latin name of the strain is the fusarium fujikuroi. The strain is obtained through screening and mutagenesis from original gibberellins A4+7 production strains. A method for producing gibberellins A4 through fermenting the fusarium fujikuroi comprises the following steps of firstly performing plate culture to activate the strains; and then, performing seed culture and fermentation culture to obtain target products of the gibberellins A4. The fermentation level reaches 1447mg / L; meanwhile, the byproducts of gibberellins A7 account for less than 4 percent of the fermentation mixed products (GA4+7); the product condition is stable; and the method is suitable for industrial production.

The invention belongs to the field of genetic engineering, and discloses a receptor protein kinase gene and an expression vector and application thereof. The complementary deoxyribonucleic acid (DNA) sequence of the receptor protein kinase gene TaRLK-B is a sequence identifier number 1 (SEQ ID N0.1), and the coded amino acid sequence of the receptor protein kinase gene TaRLK-B is an SEQ ID NO.2. The gene is from a wanghuibai variety of common wheat (Triticum asetivumL.), and is reported in the wheat for the first time. The TaRLK-B is induced by a gibberellic disease in wanghuibai one variety of the wheat capable of resisting the gibberellic disease to be enhanced in expression, and the expression level of the TaRLK-B is much higher than that in Alondra's one variety of the susceptible wheat. The gene is inserted in pAHC25 to obtain an overexpression vector to convert yang wheat 158 one variety of the wheat affected by the gibberellic disease. After identification of T0-generation gibberellic disease resistance, results show that overexpression of the TaRLK-B can improve resistance of varieties of wheat affected by the gibberellic disease on the gibberellic disease.

The invention discloses a quinoline compound and a preparation method and application thereof to prevention of plant diseases. The test result shows that the compound has the excellent prevention effects on plant diseases caused by cotton oxysporum, gibberella saubinetii, sclerotinia scleotiorum, rhizoctonia solani and rice blast and pumpkin powdery mildew and cucumber powdery mildew. The quinoline compound is simple in preparation technology, the raw materials are cheap and easy to obtain, the product purity is high, and the quinoline compound can be hopefully developed as a new bactericide.

The present invention provides one new gene MgKIN17 from Pyricularia gisea, and the gene features that it has the amino acid sequence from the site 1 to the site 3544 in the SEQ ID No. 1, the cDNA with the nucleotide sequence of SEQ ID No. 2, the promoter with the nucleotide sequence of SEQ ID No. 4, and coded protein with the amino acid sequence of SEQ ID No. 3. The insertion mutation and gene complementation of the present invention shows that the destruction of the gene leads the formation of Pyricularia gisea infecting organ and reduced lesion capacity of rice. Wheat bakanae fungus, corn stalk rot and other pathogenic fungus have homogenous protein with homogeneity over 40 % to MgKIN17 and maybe similar biological function. The gene of the present invention and its protein expression and modification may be used as the target site for designing and screening antifungal medicines.

The invention discloses a dihydroagarofuran type sesquiterpene compound, the preparing method of the compound, and the application of the compound in preparing anti-microbial pesticides and relates to the field of medicinal chemistry. According to the application of the novel sesquiterpene compound in anti-microbial pesticides, the structure of the adopted sesquiterpene compound is shown in the figure of the abstract and is named 1beta-acetyl-6alpha-(5-carboxyl-N-methyl-2-pyridone)-8beta-nicotinoyl-9alpha-benzoyl-beta-dihydroagarofuran. The compound is characterized in that the stem leaves of the plant Monimopetalum Chinese Rehd. are used as raw materials, and then the compound is obtained through extraction and separation with the natural product chemistry method. As a pesticide, the compound has a remarkable resisting effect on pests including plutella xylostella, cabbage caterpillar, corn armyworm, prodenia litura, argyrogramma agnate, pea aphids, rice-stem borer, rice tryporyza incertulas and henosepilachna vigintioctomaculata. As a sterilant, the compound has a remarkable resisting effect on sclerotinia sclerotioru, rhizoctonia cereal, fusarium graminearum and botrytis cinerea person.

The invention discloses a wheat scab control method. The method includes 1, preparing selenium solution; 2, on a sunny day, spraying the selenium solution with the certain concentration on wheat in flowering period for 1 to 3 times through a spray device, allowing the wheat to serve as a treatment group, and spraying water on wheat serving as a control group; 3, on a sunny day for 3 to 5 days after flowering, namely in the early filling period, spraying the selenium solution on the treatment group through the spray device for 1 to 3 times, and spraying water on the control group; 4, recording the gibberella expanding speed on panicle portions of the treatment group and control group; 5, recording the diseased panicle rates of the treatment group and control group after 21 to 25 days after flowering; 6, recording grain infection rate after harvesting. The gibberella expanding speed on the panicle portions and the grain infection rate can be decreased significantly, and the wheat nutritional value can be increased and human, animal and plant health can be promoted through proper application.

The invention discloses 2,8-bis(trifluoromethyl)quinoline 4-modified derivatives, a preparation method thereof and an application of the derivatives in prevention and treatment of plant diseases. Thetest results show that the compounds have excellent control effect on sclerotiniasclerotiorum, rhizoctonia solani, wheat phytoalexin, pyriculariaoryzae, phyllostictazeae, botrytis cinerea, watermelongummy stem blight and cotton fusarium wilt. The derivatives of the invention are simple in preparation process, low in price and easy to obtain raw materials, high in product purity and wide in bactericidal spectrum, and are expected to be developed into a new bactericide.

The invention relates to the genetic engineering field, especially to a novel gibberella Gibberela.sp. F75 with a preservation number CGMCC No.2499, and an acidic alpha-galactosidase Aga-F75 of antiprotease from said strain, further a gene thereof and a recombinant vector containing said gene. The amino acid sequence of the alpha-galactosidase Aga-F75 is shown as SEQ ID NO.1. The enzyme has a proper action pH value, a stronger protease resistance and a better hydrolysis ability for various substrates and is used for the feed and food industries as a feed or food additive agent.

The invention provides a polysubstituted pyrido (4, 3-d) pyrimidine compounds with bactericidal activity and a synthesis method thereof. The structural general formula of polysubstituted pyrido (4, 3-d) pyrimidine compounds of the invention is showed in general formula I which includes three compounds as showed in general formulas I-1, I-2 and I-3; the proposed structure of general formula I-1 is the development and complement of Patent CN 100335481C while no relevant coverage on the structure of the general formulas I-2 and I-3 is released currently. The experiments indicate that the compounds with the general formula I can obviously inhibit various pathogenic bacteria such as fusarium oxysporum vasinfectum, rhizoctonia solani, botrytis cinera pers, gibberella saubinetii, ring rot of apple, cotton anthracnose, cucumber timberrot, cucumber target leaf spot, scab of cucumber, pepper phytophthora blight, etc., and can be used as the active ingredient of bactericides.

The invention discloses a rice endotrophic azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on fusarium graminearum and a purpose thereof. The rice endotrophic azotobacter is Burkholderia sp. GDSD125 and has the preservation number being CGMCC No.5038 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD125 CGMCC No.5038 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.

The invention discloses a hydroxyquinoline compound as well as a preparation method and application thereof in preventing and treating agricultural diseases. Testing results show that the compound hasremarkable activity upon corn leaf fulvia fulvum, cotton oxysporum, potato rhizoctonia solani kuhn, didymella bryoniae, fusarium oxysporum, wheat gibberella, rice blast, botrytis cinerea, lycium barbarum anthracnose, cotton anthracnose, sclerotinia sclerotiorum, pepper phytophthora blight, potato late blight, rhizoctonia solani, melon bacterial fruit plaque bacteria, grape meloidogynosis bacteria, Chinese cabbage erwinia carotovora, kiwi fruit anabrosis bacteria, pseudomonas lachrymans, ralstorinia solanacearum, rice bacterial leaf blight and rice bacterial stripe bacteria. The hydroxyquinoline compound is simple in preparation process, cheap and easy in raw material obtaining, high in product purity, good in bioactivity and wide in sterilization spectrum and has the potential of being developed as new fungicides.

The invention discloses a Yangbi walnut germin-like protein gene JsGLP1 and an application thereof. The nucleotide sequence of the JsGLP1 gene is as shown in SEQ ID NO:1, and is capable of encoding germin-like protein. Through functional genomics related technological research, the gene JsGLP1 is proved to have the function of enhancing plant fungal infection resistance; the antifungal gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for over expression; the transgenic tobacco plant is strong in the in-vitro antifungal activity; and JsGLP1 over-expression transgenic tobacco has a significant inhibitory effect on the growth of sclerotinia sclerotiorum, beaded gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

The invention discloses a pathogenesis-related protein 1 family gene PnPR1-2 of panax notoginseng. The nucleotide sequence of the pathogenesis-related protein 1 family gene PnPR1-2 is as shown in SEQ ID No. 1. The pathogenesis-related protein 1 family gene PnPR1-2 comprises protein encoded with an amino acid sequence as shown in SEQ ID No. 2. Related technological researches of functional genomics prove that the PnPR1-2 gene is capable of increasing the fungus resistance of plants; when the PnPR1-2 gene is built onto a plant expression carrier and transferred into tobacco for overexpression, the transgenic tobacco plant is high in in-vitro antifungal activity, and the overexpression PnPR1-2 transgenic tobacco has an evident inhibiting effect on the growth of various fungi such as Fusarium solani, Verticillium Fusarium, beaded gibberella and Alternaria panax.

The invention discloses application of a Yangbi big-bubble walnut transcription factor gene JsWRKY1. The JsWRKY1 gene is proved to have a function of enhancing the fungal disease resistance of a plant through technical research related to functional genomics. The antifungal gene JsWRKY1 is established to a plant expression vector and transferred into a tobacco for overexpression, and a transgenic tobacco plant has very high in-vitro antifungal activity, and a transgenic tobacco subjected to JsWRKY1 overexpression achieves obvious inhibiting effect on the growth of botryosphaeria dothidea, moniliform gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

The invention belongs to the application field of biology engineering technology, and particularly relates to a method of immobilizating gibberella CA3-1(Gibberella intermedia) with a preserved number of CGMCC No.4903 and using the gibberella for biological transformation to prepare nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid includes: immobilizing the gibberella CA3-1, adding a proper amount of 3-cyanopyridine, and choosing an optimum reaction condition for immobilizing thalluses to obtain a product which is the nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid strengthens tolerance of the thalluses to substrates after the thalluses are immobilized, improves production capacity of unit thalluses, simplifies separation processes of products greatly, increases reutilization times obviously, and provides a foundation for achieving technology of performing a biological transformation method by using the gibberella to prepare the nicotinic acid.

The invention discloses a method for breeding gibberellic acid high-producing strains by performing low-energy ion induced mutation on gibberella. The method comprises the following steps: firstly, preparing a protoplast suspension of 108-109 / mL by use of a gibberella strain and sterile water; applying the protoplast suspension to a culture dish for air drying; injecting a pulsed nitrogen ion beam into the air-dried protoplasts to induce the mutation of the protoplasts; adding the mutated protoplasts to sorbitol solution ice bath for suspending, and then applying the suspension to a PDAS regeneration medium plate for cultivation; performing inoculation of the well growing individual strains into a shake flask filled with a liquid fermentation culture medium for shake cultivation, thereby obtaining the gibberellic acid high-producing strains. The method for breeding the gibberellic acid high-producing strains by performing low-energy ion induced mutation on the gibberella has the advantages that the mutation of the gibberella is induced in a pulsed nitrogen ion beam injection manner and the high-producing mutant gibberella strains can be bred; according to the method, the direct mutation rate of the mutant strains can be 10%-40%, and the fermentation titer is increased by 30-90% in contrast with that of the original strain.

The invention relates to an application of a lilium regale germin protein gene LrGLP1. The nucleotide sequence of the LrGLP1gene is shown as SEQ ID NO:1, a germin-like protein is encoded, and according to the invention, functional genomics related technical researches prove that the LrGLP1gene has a function of improving the fungal infection resistance of plants. An antifungal LrGLP1 gene in the invention is built into a plant expression vector, and transferred into a tobacco to be subjected to excessive expression, so that a transgenic tobacco plant has an extremely strong in-vitro antifungal activity. LrGLP1overexpressed transgenic tobaccos have an obvious inhibitory effect on the growth of alternaria alternate, sclerotinia sclerotiorum and catenuliform gibberella.

The invention belongs to the field of genetic engineering and discloses a proline-rich protein gene as well as an expression vector and application thereof. A cDNA (complementary Deoxyribonucleic Acid) sequence of Ta-PRP3 is SEQ ID NO.1, and an encoded amino acid sequence is SEQ ID No.2; the gene is from a common weat gibberellic disease resistant variety wnghuibai (Triticum Aestivumcv.Wanghuibai); the expression is enhanced in the wanghuibai due to the induction of gibberella; the gene transforms an infected wheat variety Yangmai 158 through particle bombardment; the result shows that the excessive expression of the Ta-PRP3 can be used for enhancing the resistance of the Yangmai 158 to the gibberellic disease, therefore the Ta-PRP3 is expected to be applied to the genetic engineering breeding; and the fusarium head blight resistance of the wheat is expected to be enhanced by introducing the Ta-PRP3 into the wheat varieties which are easily infected by the fusarium head blight.

The invention discloses a method for identifying wheat scab extension resistance through wheat ear top double-flower inoculation. A prepared gibberella spore liquid is inoculated into bilateral florets of a sixth spikelet at the top of a wheat ear by utilizing a double-flower instilling method in a wheat flowering stage, a browning condition of inoculated spikelet is recorded, a diseased spikeletrate of infected spikelet at the lower part of the inoculated spikelet after inoculation is counted, and wheat scab extension resistance is dynamically evaluated. According to the inoculation method,a diseased part mainly expands downwards from the sixth spikelet on the top, the diseased spikelet rate at the lower part of the sixth spikelet is counted, diseased parts are consistent, the problem that identification reliability is affected due to the fact that white spikes are prone to being withered through a traditional single-flower instillation method is solved, and the extension resistanceof varieties can be reflected more accurately.

The invention discloses a plant extract for promoting seed germination, and a preparation method and the application thereof, and relates to the field of the seed cultivation. The preparation method comprises the following steps: cultivating gibberella, adjusting the pH value and filtering to obtain a filter cake and filtrate containing microorganism mycelium; washing the filter cake, combining the washing liquor and the filtrate; mixing with an organic solvent at 5-10 DEG C after evaporating and concentrating in vacuum; separating the mixture into an aqueous phase and a first organic phase, and removing the first organic phase; extracting the aqueous phase by adopting the organic solvent to obtain the second organic phase; combining the first organic phase and the second organic phase toform a concentrated organic solution; heating at 60-70 DEG C, stirring until the precipitation of the solid substance is terminated; cooling to a room temperature, filtering to obtain the precipitate;washing the precipitate by using the organic solvent, and drying to obtain a grey-white solid; and dissolving the grey-white solid to obtain the plant extract. The injury on the seed germination by excessive gibberella concentration is solved, the plant extract and the ordinary pesticide can be mixed and have a mutual synergy effect; the preparation method is simple, convenient, economical and labor-saving.

A substituent thieno [3', 2':5,6] pyrido [4,3-d] pyrimidine-4(3H)-one with bactericiding activity and its preparing process is also disclosed. It can be used as the bactericide with high suppression activity to cotton wilt fungus, the sheath and culm blight bacteria of rice, gray mold of cucumber, etc.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats) / Cpf1 gene editing system as well as a construction method and the application thereof in gibberella. The system is a skeleton plasmid pCpf1Ff-DR, and comprises an Fncpf1 gene, a functional crRNA gene, a promoter pGPD, a promoter pTRPC, a strong RNA polymerase III type promoter 5srRNA and a hygromycin selection marker hph. The system is high in efficiency, strong in universality, easy to operate and capable of being rapidly applied to filamentous fungus gibberella, and the gene editing system can be used for achieving knockout of the bicarpin gene cluster as long as 17kb in the gibberella. According to the method, large-fragment gene knockout is achieved in gibberellin for the first time, it is found for the first time that knockout of the bicarpin gene cluster is beneficial to increase of the yield of gibberellin, and the method is expected to be further used for transforming gibberellin industrial bacteria, shortening the time of bacterial strain upgrading and providing powerful guarantee for increasing the yield of gibberellin and increasing economic benefits.

The invention discloses a method for identifying wheat scab resistance. The method comprises the following steps: inoculating wheat spike base internodes with a gibberella spore liquid after wheat heading, recording the browning condition of an inoculation point, judging whether the inoculation succeeds or not, respectively counting the scab rate on the 7th day, the 14th day and the 21st day afterthe inoculation, and dynamically evaluating the wheat scab resistance. The method has the greatest advantages of fastness and accuracy in observation of the disease condition and identification of the variety resistance, and solves the problems of difficult control of the spore inoculation amount, slow disease incidence, large simultaneous inoculation of the same variety in spike rate difference,high stem corrosion possibility and identification reliability influence of a stem injection method. Compared with the ear base stalk injection method, the method of the invention has the advantagesof consistent and small dosage of the spore liquid at the inoculation part, improvement of the consistency of disease attack, high inoculation efficiency, low cost, obvious phenotypic difference between variety resistance and variety susceptibility, and facilitation of common technicians can judge the gibberella disease resistance of wheat varieties.

The invention belongs to the field of chemical synthesis medicine, and particularly relates to a tetraniliprole compound with antibacterial activity as well as a preparation method and application thereof. The structure formula of the tetraniliprole compound with antibacterial activity is shown as the following formula, wherein R1 is H or F; R2 is H or alkyls of C1-C12; R3 is substitutional-group-free or F, Cl, Br, methyl, -CH2OH, CO2Et or NO2-containing or ester group substituted aromatic ring, pyridine, pyrimidine, thiazole or parazole. The tetraniliprole compound has the inhibition activityon penicillum italicum, gibberella saubinetii, gloeosporium musarum, lychee anthrax, tomato blight and magnaporthe oryzae. The tetraniliprole compound provided by the invention has good sterilizationand antibacterial activity. The formula is shown in the description.

The invention provides a polysubstituted pyrido (4, 3-d) pyrimidine compounds with bactericidal activity and a synthesis method thereof. The structural general formula of polysubstituted pyrido (4, 3-d) pyrimidine compounds of the invention is showed in general formula I which includes three compounds as showed in general formulas I-1, I-2 and I-3; the proposed structure of general formula I-1 isthe development and complement of Patent CN 100335481C while no relevant coverage on the structure of the general formulas I-2 and I-3 is released currently. The experiments indicate that the compounds with the general formula I can obviously inhibit various pathogenic bacteria such as fusarium oxysporum vasinfectum, rhizoctonia solani, botrytis cinera pers, gibberella saubinetii, ring rot of apple, cotton anthracnose, cucumber timberrot, cucumber target leaf spot, scab of cucumber, pepper phytophthora blight, etc., and can be used as the active ingredient of bactericides.

The invention provides a fullerene / gutta-percha ultraviolet absorption film material and a preparation method and application thereof, and belongs to the technical field of composite materials. The fullerene / gutta-percha ultraviolet absorption film material provided by the invention comprises a film matrix and fullerene dispersed in the film matrix, wherein the film matrix is formed by gutta-percha, and the fullerene is C60. The film material provided by the invention is obtained by compounding fullerene C60 and gutta-percha, wherein the fullerene C60 has good ultraviolet absorption capacity,and the gutta-percha has good biodegradability and is environment-friendly, so that the film material obtained by compounding the fullerene C60 and the gutta-percha retains respective characteristics,is good in biodegradability and has a wide ultraviolet absorption band, the ultraviolet absorptivity in the wave band of 200-400 nm almost reaches 100%, and a good prevention and control effect on the gibberella saubinetii is achieved.

The invention relates to a genetically engineered bacterium for producing gibberellin GA3 at high yield, a construction method and application. According to the present invention, by enhancing the gene expressing the nitrogen source regulatory factor AreA and the gene expressing the global regulatory factor Lae1 in the gibberella zeylanica, the positive regulation effect of the gibberella zeylanica on the related gene in the gibberellin anabolism path is enhanced, the transcriptional level of the related gene is improved, and the accumulation of gibberellin GA3 is improved. According to the recombinant gibberellin GA3 high-yield recombinant gibberellin GA3 provided by the invention, compared with wild gibberellin GA3, the transformed recombinant gibberellin GA3 has the advantages that the overall transcriptional level of a GA gene cluster is enhanced, the synthesis capability of a metabolic process is improved, the transcriptional inhibition of GA3 synthesis related genes is weakened, a carbon source substance and a nitrogen source substance can be better utilized for producing gibberellin GA3, and the yield of gibberellin GA3 is improved. The level of the transformed strain with the optimal performance for producing gibberellin GA3 through fermentation is improved from 2 g / L to 3.25 g / L compared with the original strain, the production capacity of gibberellin GA3 is remarkably improved, and the strain can be applied to large-scale production of gibberellin GA3.

The invention discloses a method for detecting the active thallus amount of Fusarium fujikuroi in gibberellin fermentation broth, and belongs to the technical field of fermentation. The method comprises the steps: (1) the to-be-detected fermentation broth is taken and mixed with a pre-blended XTT-menadione color-producing reagent to form a reaction system, and to-be-detected broth is prepared through incubation; (2) the light density OD450 of the to-be-detected broth at 450 nm is measured through a spectrophotometer; and (3) the measured OD450 value is directly used for representing the activethallus amount of the Fusarium fujikuroi in the to-be-detected fermentation broth, or the dry cell weight in the to-be-detected fermentation broth is calculated according to a formula to represent the active thallus amount of the Fusarium fujikuroi. According to the detecting method, interference of the physical properties of insoluble substances, dead cell residues and filamentous thallus in thegibberellin fermentation broth to detection is greatly reduced, the thallus amount of the Fusarium fujikuroi in the gibberellin fermentation broth can be accurately reflected, operation is easy and convenient and easy to implement, cells do not need to be washed, the detection sensitivity is high, and repeatability is achieved.