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Novel fusarium fujikuroi and method for producing gibberellins A4 through fermenting fusarium fujikuroi

A technology of Fusarium fusarium and gibberellin, which is applied in the field of microbial fermentation, can solve the problems that are not conducive to the control of production costs of enterprises, the production scale is not easy to enlarge, and the strains and technical methods have not been reported.

Active Publication Date: 2016-04-27
ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method makes the downstream separation process complicated, which is not conducive to the control of the production cost of the enterprise
However, other separation methods such as high performance liquid chromatography and thin layer chromatography have disadvantages such as poor control of separation speed, expensive equipment, and difficult scale-up of production.
gibberellin A for non-industrial application 4 (Bioche.AndBiophy.Res.Comm.91 (1), 1979) found that the pathogen SphacelomaMonihoticola of cassava scab only produces gibberellin A 4 , but gibberellin A 4 The yield is only 400μg / L fermentation liquid, can't reach the lowest level of industrial production, and no further report has been seen so far
[0007] High level of direct production by fermentation method suitable for industrial production to obtain high content of gibberellin A 4 The strains and technical methods of the product have not been reported

Method used

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  • Novel fusarium fujikuroi and method for producing gibberellins A4 through fermenting fusarium fujikuroi
  • Novel fusarium fujikuroi and method for producing gibberellins A4 through fermenting fusarium fujikuroi
  • Novel fusarium fujikuroi and method for producing gibberellins A4 through fermenting fusarium fujikuroi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Seed culture: The composition of the seed medium is (g / L): 40 sucrose, 12 soybean powder, NH 4 NO 3 1.2, MgSO 4 ·7H 2 O1.5 and KH 2 PO 4 1.5, liquid volume 25 / 250mL. After sterilization, use a sterile toothpick to scrape off a single activated colony from the plate, put it into a test tube filled with 2mL water and about 20 sterile glass beads with a diameter of 0.3mm, shake vigorously for 10min, break up and mix well, and absorb 0.5mL Cultivate in the seed medium for 48 hours at a temperature of 30° C. and a shaker rotation speed of 200 rpm to obtain a seed liquid.

[0033] Before seed cultivation, connect the strains to PDA medium. The composition of PDA medium is (g / L): potato juice 150~250, glucose 10~30 and agar 15~20, at pH 6.0~7.0, temperature Under the condition of 24-30°C, cultivate for 3-6 days to obtain activated Gibberella colonies.

[0034] (2) Fermentation culture: The composition of the fermentation medium (formula 1) is (g / L): starch 120, soyb...

Embodiment 2

[0037] (1) Seed culture is the same as in Example 1.

[0038] (2) Fermentation culture: The composition of the fermentation medium (formula 2) is (g / L): lactose 120, soybean cake powder 10, corn steep liquor powder 30, K 2 SO 4 2.0, MgSO 4 ·7H 2 O1.0 and ZnSO 4 ·7H 2 O5×10 -3 , liquid volume 50 / 500mL. At the time of inoculation, 2 mL of the seed solution was added to the fermentation medium, and cultured for 9 days at 31° C. with a shaker rotation speed of 200 rpm to obtain a fermentation solution.

[0039] (3) Product detection: the detection method is the same as in Example 1, and the detection result: gibberellin A 4 870mg / L, gibberellin A 7 19mg / L, gibberellin A 4 accounted for GA 4+7 The total amount is 97.9% (weight ratio).

Embodiment 3

[0041] (1) Seed culture is the same as in Example 1.

[0042] (2) Fermentation culture: The composition of the fermentation medium (formula 3) is (g / L): glucose 120, soybean cake powder 10, corn steep liquor powder 30, K 2 SO 4 2.0, MgSO 4 ·7H 2 O1.0 and ZnSO 4 ·7H 2 O5×10 -3 , liquid volume 50 / 500mL. At the time of inoculation, 2 mL of the seed solution was added to the fermentation medium, and cultured at 31° C. with a shaker rotation speed of 200 rpm for 8 days to obtain a fermentation solution.

[0043] (3) Product detection: the detection method is the same as in Example 1, and the detection result is: gibberellin A 4 761mg / L, gibberellin A 7 28mg / L, gibberellin A 4 accounted for GA 4+7 The total amount is 96.5% (weight ratio).

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Abstract

The invention relates to novel fusarium fujikuroi. A strain is collected in CGMCC (China General Microbiological Culture Collection Center) of China microbe collection management committee on July 15, 2015; the collection number is CGMCC No.11101; the classification name is the fusarium fujikuroi; and the Latin name of the strain is the fusarium fujikuroi. The strain is obtained through screening and mutagenesis from original gibberellins A4+7 production strains. A method for producing gibberellins A4 through fermenting the fusarium fujikuroi comprises the following steps of firstly performing plate culture to activate the strains; and then, performing seed culture and fermentation culture to obtain target products of the gibberellins A4. The fermentation level reaches 1447mg / L; meanwhile, the byproducts of gibberellins A7 account for less than 4 percent of the fermentation mixed products (GA4+7); the product condition is stable; and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation and relates to the production of gibberellin A by fermentation of a new fusarium fusarium 4 method, especially the by-product gibberellin A in the fermentation broth 7 gibberellin A 4+7 Below 4%. Background technique [0002] Gibberellin A involved in the present invention 4 , its structural formula is as follows: [0003] [0004] Gibberellins (Gibberellins, GA) are tetracyclic diterpenoids, which are a kind of endogenous plant regulatory substances, which widely exist in plants. So far, 129 kinds of such substances have been isolated and identified. This type of substance has a significant regulatory effect on seed germination, stem and leaf growth, bolting, fruit setting and tuber formation, and is widely used in the planting and production of grain, vegetables and fruits. [0005] Currently, gibberellin A 3 gibberellin A 4+7 It is the main gibberellin product on the ma...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P27/00C12R1/77
Inventor 吴烨飞李震宇
Owner ZHEJIANG QIANJIANG BIOCHEMICAL CO LTD
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