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Bacillus subtilis NB12, as well as culture method and application thereof

A technology of Bacillus subtilis and microbial strains, which is applied in the field of Bacillus subtilis NB12 and its cultivation, can solve problems such as affecting food safety, pollute the environment, and drug resistance of pathogenic bacteria, and achieve the effect of good control effect.

Inactive Publication Date: 2012-05-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and treatment of the disease mainly uses chemical pesticides (fungicides), and most of the fungicides are systemic agents with a single action site. Long-term use will cause the pathogens to develop drug resistance and pollute the environment. will affect food safety

Method used

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  • Bacillus subtilis NB12, as well as culture method and application thereof
  • Bacillus subtilis NB12, as well as culture method and application thereof
  • Bacillus subtilis NB12, as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Screening and Identification of Bacillus subtilis NB12

[0019] 1. Screening of bacterial strains

[0020] (1) Collection of soil samples

[0021] In this experiment, soil samples were collected from the orchard of the farm of South China Agricultural University. According to the topography of the collection site, the 5-point method was used for sampling. The 5 samples were evenly mixed, put into a sterilized sealed polyethylene bag, and brought back to the laboratory for immediate separation.

[0022] (2) Preparation of sample suspension

[0023] Add the soil sample into sterile water and shake vigorously. Sample: water = 1:9 (mass to volume ratio), for example: add 10 g of soil to a conical flask filled with 90 mL of sterilized water to obtain a suspension with a concentration of 10 -1 , prepare 5 test tubes containing 9mL sterilized water for each sample, and dilute the above concentration to 10 by 10-fold gradient dilution method -1 The suspen...

Embodiment 2

[0045] Example 2 The inhibitory effect of Bacillus subtilis NB12 on plant pathogenic bacteria

[0046] 1. Control experiment of Bacillus subtilis NB12 on rice sheath blight at seedling stage

[0047] (1) Test strains of rice sheath blight

[0048] The rice sheath blight strain GD-118 used in this experiment is a highly pathogenic strain preserved in our laboratory.

[0049] (2) Control experiment of rice sheath blight at seedling stage

[0050] Sow the susceptible rice variety Wuyujing No. 3 for germination in a large white bread box, 6 rows per pot, 10 plants per row, and 10 pots in total. When the rice seedling grows to 3 leaves and 1 heart, the grain inoculum is used for inoculation, that is, the rice grain is flatly spread on the PDA plate connected with the mycelium block of the strain GD-118, and after the mycelium is covered with the grain, each plant The base of the seedlings was inoculated with 2 grains of rice with bacteria. The spraying treatment was car...

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Abstract

The invention discloses bacillus subtilis NB12, as well as a culture method and an application thereof. The strain with a preservation number of CGMCC No.5383 is preserved in China General Microbiological Culture Collection Center on October 24, 2011. Researches prove that: the strain has strong inhibition effects to rhizoctonia solani, colletotrichum musae, pyricularia grisea, fusarium graminearum, fusarium oxysporum f.sp.cubense, peronophythora litchi, ustilago scitaminea, fulvia fulva, xanthomonas oryzae and xanthomonas oryzae pv.oryzicola, is a broad-spectrum antagonistic strain, can be used for prevention and control of various plant diseases, has a particular good prevention and control effect to the rice sheath blight and damping off in seedling phase of cowpea, has the advantages of harmlessness to human and livestock, greenness and safety, good environment compatibility, difficult resistance generation and the like, can be used for commercial development, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection (microbial pesticides), and in particular relates to a bacillus subtilis NB12 and its cultivation method and application. Background technique [0002] Plant diseases caused by plant pathogenic bacteria (including fungi, bacteria, viruses, etc.) have caused significant economic losses to agricultural production. Rhizoctonia solani ( Rhizoctonia solani Kühn) is an economically important phytopathogenic fungus. In my country, rice sheath blight caused by Rhizoctonia solani has ranked first among the three major diseases of rice. In addition to causing sheath blight of rice and corn, the fungus can also cause blight at the seedling stage of many other plants (vegetables, fruit trees, forest trees, flowers, etc.). At present, the prevention and treatment of the disease mainly uses chemical pesticides (fungicides), and most of the fungicides are systemic agents with a single action site. Long-term u...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P1/00C12R1/125
Inventor 周而勋舒灿伟杨媚周登博张德涛曹琦琦
Owner SOUTH CHINA AGRI UNIV
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