108 results about "Wheatear" patented technology

The invention discloses a method for extracting wheatear morphological parameters, which comprises the following steps: S1, adopting a reference object method to collecting a front view and a side view of the wheatear, and carrying out pre-process on the collected front view; S2, segmenting the pre-processed front view to obtain an image of the reference object and an image of the wheatear and calculate the pixel length ratio; S3, segmenting wheatear body and wheat awn of the wheat in the image of the wheatear to obtain images of wheatear body and wheat awn; S4, aiming at the image of wheatear body, adopting principal component analysis to find the axile wire and the outline of the wheatear body, calculating the length of the wheatear, and determining the shape of the wheatear; and S5, aiming at the wheat awn image, adopting a template matching method to count the number of the wheat awn and calculating the average length of the wheat awn. The method of the invention improves the comprehensiveness of the parameters extracting of the wheatear and the acquiring efficiency, and improves the objectivity and veracity of the wheatear morphological parameters extracting.

The invention discloses an ecological breeding method of efficient polyculture of pseudorasbora parva, crab larva and loach. The method comprises the steps of: pond conditions, disinfection, breeding water preparation, grass planting, fry breeding, water level adjustment, water quality adjustment and control, feeding management, disease control, daily management and the like. The breeding method provided by the invention focus on the breeding of crab larvae; the pseudorasbora parva is mainly used as cleaners for residual feeds, so that the pollution to water quality can be greatly reduced, the water quality can be improved, the growth of crab larvae and loaches is promoted, and meanwhile, blue-green algae outbreak in the crab larva pond can be biologically controlled by the polyculture of the pseudorasbora parva; the loaches play a role in loosening the soil, and eliminates hazardous substances in the soil in time, so as to avoid the water quality becoming bad due to the high temperature in summer, in addition, loaches do not need additional feeding and management, are beneficial supplement to the breeding of the crab larvae, and thus the pond benefit is greatly increased.

The invention relates to detection primers for molecular markers in close linkage with a major QTL (Quantitative Trait Locus) of wheat ear length and an application of the detection primers. The detection primers for the molecular markers include an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.1, of the molecular marker XWMC112 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.2, of the molecular marker XWMC112, and an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.3, of the molecular marker XCFD53 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.4, of the molecular marker XCFD53. The molecular marker XWMC112 and the molecular marker XCFD53 are studied and found for the first time, and can be used for quickly determining whether a wheat variety or strain has the QTL of the ear length, and thus quickly screening out the wheat variety or strain having the QTL for increasing the ear length, and therefore, the breeding progress of a high-yield wheat variety can be accelerated.

The invention discloses an identification method for sprouting conditions of wheat ears, which comprises the following steps: S1 collecting hyperspectral images of the wheat ears; S2 performing pretreatment to the hyperspectral images of the wheat ears; S3 synthesizing the hyperspectral images of the wheat ears after the pretreatment to obtain red green blue (RGB) images; S4 analyzing areas of the RGB images which operators are interested in, and obtaining average spectra of the areas which the operators are interested in; S5 judging whether the wheat ears sprout under a characteristic wave band; S6 calculating the proportion of sprouting areas in the whole wheat ear area; S7 extracting spectral reflectivity of the sprouting areas, and judging the sprouting degree of the wheat ears; and S8 calculating the sprouting grade according to the proportion of the sprouting areas in the whole wheat ear area and the spectral reflectivity. The identification method adopts the hyperspectral image technology to perform wheat ear sprouting detection on the wheat ears normally harvested in the fields, and rapid screening of wheat ear sprouting can be achieved.

The invention discloses a method for counting seeds of a wheatear portion per wheat. The method comprises the following steps: S1, collecting a front view of the wheatear portion by utilizing a CCD camera and performing pretreatment on the collected front view; S2, carrying out mathematical morphology analysis, wherein the mathematical morphology is a new method applied to the field of image processing and pattern recognition; S3, extracting wheatear portion characteristic parameters, wherein the characteristics of the wheatear portion are extracted so as to establish correlationship between correlated characteristic parameters of the wheatear portion and the number of the seeds; and S4, performing mathematic model testing, which is for testing the correlationships between area characteristics as well as long axis characteristics of the wheatear portion and the number of the seeds so as to ensure the practicality of the two models. Through the method, mathematic models between the wheatear portion image area characteristics as well as long axis length characteristics and the number of the seeds are established through extracting the wheatear portion image area characteristics as well as long axis length characteristics, so that the number of the seeds of the wheatear portion can be accurately, rapidly and objectively counted, and compared with the manual work, the method greatly helps to improve the efficiency of seed counting.

The invention belongs to the technical field of aquatic livestock reproduction, and relates to a method for promoting the gonad maturing of pseudorasbora parva out of season. The method comprises the following steps of: (1) in the middle and lower reaches of Changjiang River, selecting pseudorasbora parva longer than 40mm and heavier than 1.0g in September to October, and carrying the pseudorasbora parva back with an oxygen bag as a parent; (2) soaking the pseudorasbora parva body in 2-3% saline solution for 5 minutes, and temporarily cultivating for 2 weeks in a circulating aquaculture system, wherein the daily feed is 5-8% of the pseudorasbora parva weight in the temporary cultivation period; (3) transferring the pseudorasbora parva into an aquarium with a circulating filtration and oxygen increasing device, wherein the density is 12 pseudorasbora parvas per aquarium; and controlling the water temperature to 22-24 DEG C, the light intensity on water surface is 400lux, and the illumination time is 16-24h / d; (4) feeding the pseudorasbora parvas in the morning and afternoon everyday; and (5) changing water once every 2 weeks, wherein 1 / 2 of the cultivation water is changed; and the physicochemical conditions of water are: the dissolved oxygen is greater than or equal to 7.5mg / L, the pH is 7.5-8.0, the ammonia nitrogen is less than or equal to 0.1mg / L, and the nitrite nitrogen is less than or equal to 0.01mg / L; and cultivating for 2 months to obtain the pseudorasbora parva with mature gonad development.

The invention discloses a molecular marker closely linked with a major gene locus of the grain weight of a wheatear. The DNA (Deoxyribose Nucleic Acid) of a wheat variety Jing 411 is subjected to PCR (Polymerase Chain Reaction) amplification by using marker primers Xcfd80.2 and Xbarc146.1, and then, amplification products are electrophoretically separated on 8% polyacrylamide gel, so that the amplification products with the molecular weights of respectively 160bp and 142bp are obtained, i.e., the molecular marker of the major gene locus QKws.cas-6A of the grain weight of the wheatear is obtained. The molecular marker of the major gene locus of the grain weight of the wheatear, provided by the invention, can be used for detecting whether a wheat variety or strain contains the major gene locus QKws.cas-6A for increasing the grain weight of the wheatear, so that the wheat variety or strain with the gene locus is rapidly screened and used for breeding, and the breeding progress of the high-yield wheat variety can be greatly accelerated.

The invention relates to a winter wheat head blight hyperspectral remote sensing monitoring method based on wheat ear scale analysis, and compared to the prior art, overcomes the defect that remote sensing monitoring on the head blight does not aim at wheat ear scale analysis. The winter wheat head blight hyperspectral remote sensing monitoring method comprises the following steps of: acquiring hyperspectral remote sensing data; carrying out data preprocessing; constructing a wheat head blight index; establishing a multiple stepwise regression model; and obtaining a remote sensing monitoring result. According to the invention, after the head blight index is constructed by utilizing a normalization ratio of a first-order differential sum in sensitive wavebands, unary linear regression and multiple stepwise regression models of the head blight index and disease severity are established, so as to implement effective monitoring on the wheat head blight and provide ideas and bases for noninvasive diagnosis on the head blight of infected wheat on the canopy scale and the field scale.

The invention discloses a method for artificially inducing field wheat scab, belonging to plant disease technical field. The method includes culturing conidiophore of wheat scab strain; preparing wheat scab strain inoculum; preparing moisture-retaining cloth; inoculating wheat scab strain inoculum, and retaining moisture; observing disease condition, recording, and performing statistics. The method retains moisture with moisture-retaining cloth, to significantly improve environmental humidity of wheat ear position and balance temperature, thereby facilitating invasion and fixation of wheat scab strain and improving field wheat scab artificial inducement effect. The method has simple implementation, no need of high input of manual labor and high-cost facilities, and applicability to wheat planting test areas.

The invention discloses a molecular marker closely linked with wheatear grain number major QTL. Wheat genome DNA serves as a template, a PCR primer pair is adopted to conduct PCR amplification, the amplificated product is subjected to ionophortic separation through polyacrylamide gel to obtain the molecular marker 2A-654918338. The invention further discloses application of the molecular marker 2A-654918338 in detecting whether a wheat variety or line contains the QTL for increasing the wheatear grain number and application of the molecular marker 2A-654918338 in molecular breeding of wheat. By means of the molecular marker, the genetic improvement progress of the wheatear grain number is accelerated, the selection efficiency and quality of the wheat variety or line are greatly improved, identification of the target gene in wheat genetic resources and breeding progenies is directly achieved, and more new wheatear grain number genetic resources are developed for wheat breeding.

The invention provides a method for counting wheat ears, which includes (1) collecting wheat ear image, (2) Converting the color space of the collected wheat ear image; (3) Image segmentation being carried out on the color space converted image, so that the content in the image is divided into three parts: wheat ear, cultivated land, wheat leaf and stem; (4) binarizing the segmented image to separate the ear from the image; (5) filtering the binary image, filtering out the binary connected area which is too large or too small, and then counting the number of wheat ears according to the binaryimage after filtering. The method of the invention establishes a direct mapping relationship from the low-level feature of an image to the number of ears of wheat contained in the image through the color feature, so that the target does not need to be segmented or detected again. This kind of method is more suitable for complex lighting and dense ears of wheat scene, and has higher computational efficiency and accuracy than image segmentation method.

The invention discloses a wheatear in-vitro culture solution formula of hybrid induced haploid embryos of wheat and maize and a preparation method thereof. The culture solution is prepared from the following compounds according to corresponding dosage: 100mg / L of 2,4-dichlorphenoxyacetic acid, 35-45g / L of cane sugar, 2-4g / L of monopotassium phosphate, 6-8ml / L of sulphurous acid, 0.5-1.5g / L of sodium tetraborate, 2-4g / L of carbamide, and 8-10mg / L of silver nitrate. The culture solution prepared by the formula is used for cultivating a wheatear which is pollinated by maize pollen and cut inside a room from a pan or a field, and inducing generation of haploid embryos. The inductivity of the haploid embryos after the formula is utilized can be improved by 10-15% in comparison with the existing wheatear culture solution under the condition of not changing all the other conditions.

The invention discloses a wheat ear-grain stripping structure for a small-scale harvester and the small-scale harvester with the same. The wheat ear-grain stripping structure comprises a rotation cylinder and an elastic threshing roller, a semicircular wire-mesh screen is further arranged below the rotation cylinder, a radial space between an inner surface of the mesh screen and an outer surface of the threshing roller is the grain diameter of 1.5-2.0 rice grains or wheat grains, and a space between two adjacent wefts on the mesh screen is the grain diameter of 2-3 rice grains or wheat grains. Grain casings of rice or ears entering the position between the mesh screen and the threshing roller are easy to separate under grinding and extruding effects of the elastic threshing roller, the rice or wheat grins are leaked to a collection tank from the mesh screen, and ear claws are discharged from the back along rotation of the rotation cylinder. The small-scale harvester with the wheat ear-grain stripping structure can be small in size, light in weight, less in energy consumption and low in cost, the small-scale harvester is applicable to use in time receiving in paddy fields, small wheat fields and hilly areas, and common peasant households can purchase the small-scale harvester due to low construction cost.

The invention provides an SNP molecular marker related to wheat ear germination resistance and application thereof, and particularly relates to the technical field of genetic breeding. An SNP locus corresponds to C / T base mutation at a wheat 4B chromosome 661498776 bp of a reference genome IWGSC RefSeq v1.0 version; and a CAPS marker is developed according to the SNP molecular marker, the CAPS marker is named as TaE3, and the TaE3 has a nucleotide sequence shown as SEQ ID NO. 5 or a nucleotide sequence shown as SEQ ID NO. 6. The CAPS marker is used for identifying wheat ear germination resistant varieties. The detection method is simple and convenient, and is used for wheat anti-PHS molecular marker assisted breeding.

The invention discloses a wheat variety scab comprehensive resistance identification method based on hyperspectral images, which takes wheat ears of different varieties infected by scab germs as research objects and comprehensively utilizes the knowledge in the fields of hyperspectral imaging technology, spectroscopy, time sequence analysis, deep learning and the like. The invention provides a resistance identification method for wheat varieties in an infection non-morbidity period, a resistance identification method for wheat varieties after morbidity, and a wheat variety scab comprehensive resistance identification method. The wheat variety scab comprehensive resistance identification method based on hyperspectral images overcomes the defects of difficult detection of resistance in an infection period, low speed and precision in manual identification operation, complex program and high cost in a chemical method, provides a quicker and more accurate method for identifying the resistance of wheat scab, and can identify the resistance of wheat varieties in a wheat scab infection non-morbidity period.

The invention discloses an erected-wheatear indica type three-line sterile line, and a cultivation method thereof. A dominant erected-wheatear mutant is obtained after an indica type three-line maintainer line is induced by radiation, an erected-wheatear gene is led to an excellent three-line sterile line in a hybrid backcross manner, the dominant erected-wheatear three-line sterile line is cultivated, a foundation is established for expanding the selection range of the variety by freely assembling and selecting the erected or partly erected indica hybrid rice between the varieties, a condition is provided for further enhancement of resistance to lodging, fertilizer tolerance and close planting of the rice, and the possibility of breakthrough of the yield of hybrid rice varieties is increased.

The invention discloses a method for artificially inducing parent pseudorasbora parva gonad synchronous development. The method comprises the steps of parent fish selecting, parent fish breeding, parent fish gonad synchronous development and the like. According to the method, through gradual temperature control, gradual lighting adjustment and appropriate feed putting, the purpose of promoting pseudorasbora parva gonad synchronous development is achieved, the problem of sperm and egg malformation caused by rapid environment changes is avoided, the brood rate and the fertility rate are improved and a technical basis is provided for pseudorasbora parva artificial breeding.

The invention discloses a compound fertilizer for selenium-enriched and chromium-enriched wheat. The compound fertilizer is prepared from the following raw materials in parts by weight: 400 to 600 parts of urea, 100 to 200 parts of ammonium sulfate, 250 to 300 parts of monoammonium phosphate, 100 to 150 parts of potassium chloride, 20 to 50 parts of humic acid, 5 to 10 parts of compound microelements, 40 to 60 parts of bentonite, 1 to 2 parts of inorganic selenium salt and 0.01 to 0.05 part of chromium trichloride. An application method of the compound fertilizer for the selenium-enriched and chromium-enriched wheat, provided by the invention, comprises the following steps: selecting a land with a deep and thick soil layer and without fertilizer leakage; taking lignite and turf as carriers and carrying out basal application in one step; separating seeds from the fertilizer. The compound fertilizer and the application method have the advantages of high wheat yield, high plant disease resistance and applicability to large-area application; fruited wheatears are relatively large and heavy and have good quality; the selenium-enriched and chromium-enriched wheat contains selenium and trivalent chromium elements required by human bodies and animals, is high in nutritional value and is good for health.

The invention discloses honey flour capable of enriching the blood. The honey flour is prepared from the following raw materials in parts by weight: 400-405 parts of wheat, 0.1-0.2 part of rose essential oil, 60-65 parts of black sesame,100-110 parts of green wheatear, 50-60 parts of tender peanuts, 60-70 parts of honey, 40-45 parts of milk, 60-70 parts of sweet potato powder, 30-35 parts of longan, 50-55 parts of grapes, 20-25 parts of jujube, 40-50 parts of brown sugar, 35-37 parts of egg yolk powder, 6-8 parts of ginseng and the like. Due to the addition of green wheatear and tender peanuts, the honey flour capable of enriching the blood disclosed by the invention is abundant in nutrient, and overflowing in fragrance, has good blood-enriching action by matching with jujube, the grape and the like, has a good blood-enriching effect by reasonably matching with extracts such as ginseng, donkey-hide glue, angelica sinensis, radix ophiopogonis and honeysuckle, is not easy to inflame, and has good life cultivation and health preservation functions.

The invention discloses a wheat grain color related CAPS marker and a detection method thereof and relates to the technical field of molecular breeding of wheat. The CAPS marker is named as KU4104-6AL, and has a sequence of SEQ ID NO. 1. A method for identifying the wheat grain color comprises the following steps: 1, extracting DNA of to-be-detected wheat genome; 2, performing PCR (Polymerase Chain Reaction) amplification on the DNA of the wheat genome by utilizing sequences such as SEQ ID NO. 2 and SEQ ID NO.3 so as to obtain an amplification product; 3, performing digestion on the amplification product by utilizing a NruI incision enzyme having a recognition sequence of TCG / CGA so as to obtain enzyme-digested products; 4, ensuring that the color of corresponding wheat grains is dark whenthe enzyme-digested products are 214bp and 212bp, and naming genotype of the corresponding amplification product as KU4104-6A-a; ensuring that the color of corresponding wheat grains is light when the enzyme-digested product is 426bp. The CAPS marker KU4104-6AL developed by the method in the invention is capable of effectively distinguishing colors of the wheat grains and is used for genetic improvement of sprouting resistance of wheatears and flour quality.

The embodiment of the invention provides an identification method and device for a winter wheatear differentiation phase. The method comprises the following steps that: according to collected parameters in a plantation area of winter wheat to be identified, obtaining the model parameter of a wheat growth simulation model, and obtaining a localized wheat growth simulation model; according to the agricultural meteorological station data of the plantation area of winter wheat to be identified, obtaining the first air temperature data time sequence of the winter wheat to be identified, and obtaining the second air temperature data time sequence of the winter wheat to be identified according to a multi-temporal remote sensing image in the plantation area of winter wheat to be identified; utilizing a data assimilation method, carrying out data assimilation on the first air temperature data time sequence and the second air temperature data time sequence, and obtaining the assimilation data time sequence of the winter wheat to be identified; and according to the assimilation data time sequence and the localized wheat growth simulation model, obtaining a wheatear differentiation phase identification result in the plantation area of winter wheat to be identified. By use of the embodiment of the invention, the wheatear differentiation phase identification on a regional scale is realized.

The invention discloses a method for increasing yield by utilizing weather conditions to increase the weight of inferior grains of winter wheat, which includes a fertilization measure for the winter wheat spike differentiation period, a dry-wet alternate irrigation measure for the winter wheat fructification period and an ABA spraying measure for the filling period after the flowering of winter wheat. According to weather conditions, the phasic development process of wheat is artificially controlled, so that the growth and development of inferior grains are controlled, uncontrollable weather resources are actively transformed into useful conditions, and by integrating weather conditions with the usage of exogenous hormone ABA in the phasic development process of wheat, the invention puts forward a complete new set of winter wheat cultivation method which can increase the weight of inferior grains of wheat and the uniformity of wheat grains.

The invention discloses a strengthening breeding method of parent fishes of pseudorasbora parva. The method includes the steps of parent fish selection, pond selection and treatment, parent fish strengthening breeding, water temperature adjustment, daily management and the like. According to the strengthening breeding method, gonad development of the parent fishes of the pseudorasbora parva can be stimulated, and the success rate of parent rearing maturity is improved; meanwhile rapid growth of the parent fishes is promoted, the survival rate of the parent fishes is improved, the problem that the parent quality and the survival rate of the pseudorasbora parva are hard to guarantee is effectively solved, and the method lays a technical foundation for artificial breeding of the pseudorasbora parva in China and has important significance for industrialization exploitation of the pseudorasbora parva.

The invention discloses an ear falling prevention whole-feed combine harvester. An upward inclined grass conveying chain is arranged in the rear of a stem poking roller, an upward inclined threshing roller is mounted at the upper end of the grass conveying chain, a screen, a conveying belt, a fan, a vibrating screen and a bottom screw conveyer are arranged on the lower portion of the threshing roller, the screen is fixed with a baffle plate matched with the grass conveying chain, the baffle close to the end middle of the screen is recessed to form a groove, a baffle plate replacement screen isfixed in the groove, a wind retaining plate matched with the fan is fixed below the baffle plate and connected with the mounting plate, a flow comb is fixed on the mounting plate, and a fixing plateis mounted between the mounting plate and the baffle plate. The combine harvester has the advantages that the threshing roller is obliquely arranged, grains are threshed, kernels and shells can be rapidly separated, a double-box four-gear structure in the grass conveying chain cannot be locked, and the combine harvester is provided with the baffle plate, so that rapeseed stems, long ears and wheatears cannot fall when the combine harvester harvests crops.

The invention provides a method for measuring the ear length and the ear number of multiple wheat plants based on image processing, and belongs to the technical field of computer image processing. Themethod solves the problem that the number of spikelet is mainly observed and counted by human eyes in the existing manual visual inspection, and is characterized by comprising the following steps: S1, acquiring a wheat graph; s2, geometrically correcting the image; s3, carryng out single-plant wheat segmentation; s4, cutting out an effective area of the length of the wheat ears; s5, calculating the ear length; and S6, calculating the spikelet number of the wheat. The method has the advantages that the labor cost and the equipment cost are saved, the working efficiency is greatly improved, andthe like.

The invention relates to an odontobutis obscurus large-size fry artificial classification separate culture method. The method comprises the steps that 1, a net cage is arranged in an indoor culture groove, oxygen increasing heads are arranged on the periphery of the indoor culture groove, a water pushing device is arrange on one side, and a water filtering device is arranged on the other side; 2,newly hatched larvae of odontobutis obscurus are put in the net cage of the culture groove; 3, the culture water depth is controlled to be 0.3+ / -0.6 m, the water temperature is controlled to be 22-28DEG C, and sewage suction and water replenishing are conducted every afternoon; 4, after hatching is conducted for 3 d, cladocerans and copepods obtained after filtering through a 60-mesh sieve serveas initial feed, after 7 d, bait mainly adopts limnodrilus and secondarily adopts the cladocerans and the copepods, and after 14 d, while the limnodrilus is used for feeding, loach and newly hatched larvae of pseudorasbora parva are additionally put; 5, after hatching is conducted for about 10 d, by taking the head width as the standard, odontobutis obscurus artificial classification separate culture is conducted, and hereafter, specification screening and classification are conducted every 10 d. Accordingly, indoor odontobutis obscurus large-size fry culture is achieved, after culture is conducted for about 50 d, for the odontobutis obscurus, the average overall length reaches about 20 cm, the head width is about 3.3 mm, and the pond survival rate reaches 90%.

The invention discloses a stamen killing device and method based on temperature sensitivity of wheat and rice pollen. The device comprises a casing, wherein a temperature control device, a fan-type heating device and a stamen killing tank for accommodating a wheat ear or rice ear are mounted in the casing from left to right; a left side plate of the stamen killing tank is a seal plate, a plurality of heat circulation channels are evenly distributed on a top plate and a bottom plate, a temperature sensor is further mounted in the stamen killing tank and connected with an input end of a temperature control device, and an output end of the temperature control device is connected with the fan-type heating device; a water spray door gap is reserved in a top door cover of the casing. The device is easy to operate and convenient to carry and kills stamens thoroughly, and the temperature is controlled accurately; temperature control accuracy plays a critical role in stamen killing effect of wheat and rice, stamen killing is not affected by weather, time and places and is time-saving, efficient and thorough, and stamen killing efficiency and accuracy of hybrid wheat and rice are improved.

The invention relates to the field of agricultural machinery and equipment and provides a wheat grain parameter measuring device for individual wheat and a threshing and measuring system for the individual wheat. The device comprises an image data processing unit, a measuring box, a case, and an image acquisition unit arranged within the case. The measuring box is detachably arranged at a lower portion of the case; a sample tray for placing the grain is provided in the measuring box; a weighing sensor is arranged between the sample tray and the measuring box; the image acquisition unit is suspended above the sample tray for collecting an image of the grain placed on the sample tray; and the image acquisition unit and the weighing sensor are electrically connected with the image data processing unit respectively. The system comprises a wheat thresher and the wheat grain parameter measuring device for individual wheat. According to the wheat grain parameter measuring device for individual wheat, and the threshing and measuring system for the individual wheat, the invention has a simple structure and is convenient to move; staff can use the device to achieve online measurement of grain parameters, so that the efficiency of wheat yield and quality assessment can be greatly improved.

The invention relates to a target gene silencing method for wheatears and grains mediated through recombination barley streak mosaic virus (BSMV), which achieves silencing of target gene expression of the wheatears and the grains by mixing an equal amount of alpha, beta, gamma, c deoxyribonucleic acid (DNA) in vitro transcript of recombination BSMV carrying target gene fragments and then vaccinating the wheatears ranging from a heading stage to a flowering stage. Compared with the existing ribonucleic acid interfere (RNAi) technical method based on transgenic wheatears and seeds, the target gene silencing method has the advantages of being easy, fast, accurate and good in repeatability.