Wheat variety scab comprehensive resistance identification method based on hyperspectral images
A technology of hyperspectral image and wheat scab, which is applied in the field of comprehensive resistance identification of wheat variety scab, can solve the problems of low precision, high cost, complex chemical method procedures, etc., and achieve the effect of improving the accuracy of detection
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[0036] The present invention will be described in detail below with reference to the drawings and specific embodiments.
[0037] (1) Wheat sample collection and processing
[0038] Sample selection: The samples in the experiment were all from the experimental farm in Liuhe District, Nanjing. Four types of healthy wheat from Wangshuibei, Zhenmai168, Huaimai, and Luomai 22 were taken from the farm, and the four wheat ears were cultivated separately. And make the corresponding mark according to the variety.
[0039] Cultivation of pathogens: ① Use potato dextrose agar (PDA) medium to cultivate Gibberella spp.: Wash potatoes, peel and cut 400g, heat them in boiling water for 5 minutes until the potatoes are soft, filter, pour the filtered juice into the pot and heat, add Agar, stir and add glucose to continue heating for 5 minutes, sealed and stored. ②Isolation and purification of pathogens: Obtain the diseased wheat grains, and then process them with "mercury-70% alcohol-sterilized wa...
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