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A method and primers for identifying sow's farrowing performance

A performance and technology for sows, applied in biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc., to achieve high accuracy, good comprehensive indicators, and increase the effect of total litter size

Active Publication Date: 2022-04-22
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on molecular breeding of reproductive performance focuses on the selection of single mutation sites, but reproductive performance is controlled by micro-effect polygenes, so the selection of single mutation sites for molecular breeding will have certain limitations

Method used

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  • A method and primers for identifying sow's farrowing performance
  • A method and primers for identifying sow's farrowing performance
  • A method and primers for identifying sow's farrowing performance

Examples

Experimental program
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Embodiment 1

[0041] The pig samples, reproductive performance data and pedigree data come from French Landrace pigs of Guangxi Shoumu Breeding Pig Breeding Co., Ltd.

[0042] 1. Pig FSHR c.532 G>A site and FSHR c. Determination of the 1166 C>T site mutation site.

[0043] (1) Collect pig ear samples, and use the DNA extraction kit to extract DNA from pig ear tissue.

[0044] (2) According to pig FSHR Gene GenBank Accession Number XM_021085881.1, design and synthesis of primers. FSHR The primers are as follows:

[0045] Table 1 FSHR Gene primer information

[0046]

[0047] (3) Using pig ear DNA as a template, using upper primers and PCR amplification system: 200 ng of genomic DNA, 5 µL of 10×PCR amplification buffer, 10 mM final concentration of dNTPs, 50 ng each of upper and lower primers, Taq DNA Polymerase 0.75mmol / L, make up the system to 50 µL with ddH2O.

[0048] PCR amplification program: pre-denaturation at 95°C for 5 min; denaturation at 95°C for 30 s, annealing at 58°C...

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Abstract

The invention discloses a FSHR A method and primer pair for joint analysis of two mutation sites to identify sow's farrowing performance. The present invention also discloses FSHR c.532 G>A and FSHR c. 1166 primers for C>T amplification and methods for using the primers to detect, analyze and identify sow farrowing performance. The primers, method and scheme can realize the early breeding of sows with high litter performance, shorten the breeding time of high-quality breeding pigs, speed up the breeding process, save the cost of breeding and feeding, and effectively increase the total litter size, live piglet size and health of pigs. Aberdeen. The method has high accuracy, simple operation and high practical application value in pig breeding.

Description

technical field [0001] The invention relates to a molecular breeding technique for two-point joint selection of reproductive performance of sows, a primer pair and the application of the result, and belongs to the field of biotechnology. Background technique [0002] In recent years, due to the impact of African swine fever, the world's pig industry has suffered huge losses, and my country's pig industry is no exception. The number and quality of reproductive sows decreased, and the price of pork increased. The traditional breeding cycle for pig reproductive performance is long. The level of reproductive performance directly affects the production efficiency and economic benefits of pig farms. The total number of litters, number of live pigs, and number of healthy pigs are three very important reproductive traits, and they are also very important economic traits. At present, the research on molecular breeding for reproductive performance focuses on the selection of single...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 蒲蕾温作晨李若岚黄越郭亮
Owner TIANJIN AGRICULTURE COLLEGE
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