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Method and primers for identifying farrowing performance of sows

A technology of sow, performance, applied in the direction of biochemical equipment and methods, determination/examination of microorganisms, DNA/RNA fragments, etc.

Active Publication Date: 2021-03-09
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on molecular breeding of reproductive performance focuses on the selection of single mutation sites, but reproductive performance is controlled by micro-effect polygenes, so the selection of single mutation sites for molecular breeding will have certain limitations

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  • Method and primers for identifying farrowing performance of sows
  • Method and primers for identifying farrowing performance of sows
  • Method and primers for identifying farrowing performance of sows

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Experimental program
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Embodiment 1

[0041] The pig samples, reproductive performance data and pedigree data come from French Large White pigs of Guangxi Shoumu Breeding Pig Breeding Co., Ltd.

[0042] 1. Pig FSHR c.532 G>A site and FSHR c. Determination of the 1166 C>T site mutation site.

[0043] (1) Collect pig ear samples, and use the DNA extraction kit to extract DNA from pig ear tissue.

[0044] (2) According to pig FSHR Gene GenBank Accession Number XM_021085881.1, design and synthesis of primers. FSHR The primers are as follows:

[0045]

[0046] (3) Using pig ear DNA as a template, using upper primers and PCR amplification system: 200 ng of genomic DNA, 5 µL of 10×PCR amplification buffer, 10 mM final concentration of dNTPs, 50 ng each of upper and lower primers, Taq DNA Polymerase 0.75mmol / L, make up the system to 50 µL with ddH2O.

[0047] PCR amplification program: pre-denaturation at 95°C for 5 min; denaturation at 95°C for 30 s, annealing at 58°C for 30 s, extension at 72°C for 30 s, a tota...

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Abstract

The invention discloses a method and primer pair for analyzing and identifying farrowing performance of sows by combining two mutation sites of FSHR. The invention also discloses primers for amplifying FSHR c.532 G> A and FSHR c.1166 C> T, and a method for detecting, analyzing and identifying the farrowing performance of the sow by using the primers. The primers, the method and the scheme can realize early breeding of high farrowing performance of the sows, shorten the breeding time of excellent breeding pigs, accelerate the breeding process, save the breeding cost, and effectively improve thetotal litter size, the live litter size and the healthy litter size of the sows. The method is high in accuracy and simple to operate, and has very high practical application value in the aspect of pig breeding.

Description

technical field [0001] The invention relates to a molecular breeding technique for two-point joint selection of reproductive performance of sows, a primer pair and the application of the result, and belongs to the field of biotechnology. Background technique [0002] In recent years, due to the impact of African swine fever, the world's pig industry has suffered huge losses, and my country's pig industry is no exception. The number and quality of reproductive sows decreased, and the price of pork increased. The traditional breeding cycle for pig reproductive performance is long. The level of reproductive performance directly affects the production efficiency and economic benefits of pig farms. The total number of litters, number of live pigs, and number of healthy pigs are three very important reproductive traits, and they are also very important economic traits. At present, the research on molecular breeding for reproductive performance focuses on the selection of single...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 蒲蕾温作晨李若岚黄越郭亮
Owner TIANJIN AGRICULTURE COLLEGE
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