36 results about "Mugil" patented technology

The invention discloses a wheat pre-harvest sprouting tolerance gene TaZFP18. The gene includes the nucleotide sequence shown as SEQ ID NO.1, or includes the nucleotide sequence shown as complementary with the SEQ ID NO.1 sequence, and the gene encodes a CCCH type zinc finger protein 18 and can serve as the molecular marker gene of a wheat pre-harvest sprouting tolerance variety. The wheat pre-harvest sprouting tolerance gene TaZFP18 is a novel major wheat pre-harvest sprouting tolerance gene, can be utilized to develop a molecular marker so as to rapidly identify whether wheat belongs to the pre-harvest sprouting tolerance variety or not and provides a new gene resources for breeding of wheat pre-harvest sprouting tolerance varieties.

The embodiment of the present invention provides a method and device for identifying the ear differentiation stage of winter wheat. The inventive method includes: obtaining the model parameters of the wheat growth simulation model according to the collected parameters in the winter wheat planting area to be identified, and obtaining the localized wheat growth simulation model ; According to the data of the agricultural meteorological station in the winter wheat planting area to be identified, the first temperature data time series of the winter wheat to be identified is obtained, and the second temperature data time series of the winter wheat to be identified is obtained according to the multi-temporal remote sensing images in the winter wheat planting area to be identified; The data assimilation method is to assimilate the first temperature data time series and the second temperature data time series to obtain the assimilated temperature data time series of winter wheat to be identified; according to the time series of assimilated temperature data and the localized wheat growth simulation model, the winter wheat to be identified is obtained Identification results of panicle differentiation stages in the planting area. The embodiment of the present invention realizes identification of winter wheat panicle differentiation stage on a regional scale.

The invention belongs to the technical field of aquatic livestock reproduction, and relates to a method for promoting the gonad maturing of pseudorasbora parva out of season. The method comprises the following steps of: (1) in the middle and lower reaches of Changjiang River, selecting pseudorasbora parva longer than 40mm and heavier than 1.0g in September to October, and carrying the pseudorasbora parva back with an oxygen bag as a parent; (2) soaking the pseudorasbora parva body in 2-3% saline solution for 5 minutes, and temporarily cultivating for 2 weeks in a circulating aquaculture system, wherein the daily feed is 5-8% of the pseudorasbora parva weight in the temporary cultivation period; (3) transferring the pseudorasbora parva into an aquarium with a circulating filtration and oxygen increasing device, wherein the density is 12 pseudorasbora parvas per aquarium; and controlling the water temperature to 22-24 DEG C, the light intensity on water surface is 400lux, and the illumination time is 16-24h / d; (4) feeding the pseudorasbora parvas in the morning and afternoon everyday; and (5) changing water once every 2 weeks, wherein 1 / 2 of the cultivation water is changed; and the physicochemical conditions of water are: the dissolved oxygen is greater than or equal to 7.5mg / L, the pH is 7.5-8.0, the ammonia nitrogen is less than or equal to 0.1mg / L, and the nitrite nitrogen is less than or equal to 0.01mg / L; and cultivating for 2 months to obtain the pseudorasbora parva with mature gonad development.

The invention discloses a strengthening breeding method of parent fishes of pseudorasbora parva. The method includes the steps of parent fish selection, pond selection and treatment, parent fish strengthening breeding, water temperature adjustment, daily management and the like. According to the strengthening breeding method, gonad development of the parent fishes of the pseudorasbora parva can be stimulated, and the success rate of parent rearing maturity is improved; meanwhile rapid growth of the parent fishes is promoted, the survival rate of the parent fishes is improved, the problem that the parent quality and the survival rate of the pseudorasbora parva are hard to guarantee is effectively solved, and the method lays a technical foundation for artificial breeding of the pseudorasbora parva in China and has important significance for industrialization exploitation of the pseudorasbora parva.

The invention discloses an inoculation identification method capable of observing the symptoms of wheat black germ disease in time. First, after wheat earing, the wheat ears are subjected to hulling treatment and the husked wheat ears are subjected to miscellaneous bacteria isolation; the pathogenic bacteria of wheat black embryo disease are inoculated in a petri dish containing PDA medium for dark culture, Obtain the pathogenic bacteria colonies after cultivation; wash the pathogenic bacteria colonies obtained, filter, centrifuge, and adjust the spore concentration to obtain the spore suspension for later use; when the wheat grains are full of kernels, spray the spore suspension on the shell-cut ears Cover the ears with a plastic bag to keep the ears moist; observe the infection of the germs on the inoculated grains and the formation of black germ disease symptoms after inoculation; count the black germ rate when the wheat is mature, and use it as the identification result of wheat black germ disease. The method of the invention can not only observe the occurrence of symptoms in time, but also make the identification result more stable and reliable; and can timely and reasonably prevent and control the wheat according to the dissemination situation.

The invention discloses a high yield and high efficiency fertilizing method for rain-fed wheat in rice stubble in the northern Jiangsu area. The method specifically comprises the following steps of: (1) additionally applying a phosphorus and potassium base fertilizer, wherein urea of the base fertilizer is reduced in dosage while the date of seeding is postponed; (2) strictly controlling a striking root fertilizer, and applying a jointing fertilizer due to seedlings and rain; and (3) generally applying and heavily applying a booting fertilizer, determining whether a flower raising fertilizer is applied according to the number of spikelets which do not bloom, and taking good fecundity of the spikelets of the ears of wheat, three green leaves obtained by heading a single stem in 25 days as a testing index whether the reviving jointing fertilizer is reasonable or not and the booting fertilizer is sufficient or not. The invention provides the fertilizing method with limiting conditions such as the date of seeding of the rain-fed wheat in rice stubble in the northern Jiangsu area is late, the seeding is slow to emerge, the rice straws are returned in full dose, surface soil moisture content in autumn and winter is relatively poor, the rice is hard to irrigate, and the fertilizer-retaining ability of the soil is poor. The method has the major effects of being large in spike, full in grain and high in yield and overcomes the defects that in the prior art, the base fertilizer has more striking root fertilizer and jointing fertilizer and the booting fertilizer is severely insufficient.