Molecule seed breeding method of wheat with ear sprouting resistance

Abstract

The invention discloses a molecule seed breeding method of wheat with ear sprouting resistance, and relates to the technical field of seed breeding of wheat. The molecule seed breeding method comprises the following steps of (1) by using a sequence of a VplB gene of Wanxian white wheat variety with ear sprouting resistance as a template, blasting in GenBank, so as to obtain a BAC (bacterial artificial chromosome) sequence containing the VplB gene and an upstream sequence of the VplB gene; (2) by using the BAC sequence containing the upstream sequence of the VplB gene as a template, designing primers Bi-TF and Bi-TR, and separating a promoter at the upstream 2232bp of the VplB gene of the wheat; (3) adopting a 5'-deletion method to build a transient expression vector. The molecule seed breeding method has the advantage that the gene plant expression vector is built by the VplB gene of the Wanxian white wheat with the ear sprouting resistance, and the wheat is converted by an agrobacterium-mediated method, so that the wheat has the ear grouting resistance, the economic loss due to ear sprouting of the wheat is effectively relieved, and the output and quality of the wheat are improved.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, in particular to a wheat molecular breeding method with ear germination resistance. Background technique [0002] Ear germination of wheat refers to the ear germination in rainy or humid environment before harvesting. Ear germination not only affects the yield, but also seriously reduces the processing quality and planting value of wheat. Countries such as Canada, the United Kingdom, the United States, Germany, and Australia are all suffering from ear germination hazards, and Canada and Australia are particularly serious. Winter wheat areas in the middle and lower reaches of the Yangtze River, southwest winter wheat areas, and northeast spring wheat areas frequently occur in China. The most recent wheat ear germination disasters occurred in 2008, 2009, and 2010, resulting in a sharp decline in the quality of commercial wheat in Hebei, Henan, Jiangsu, Anhui and other provinces. Seed source...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a wheat molecular breeding method with ear germination resistance, construct gene plant expression vector through Wanxian white wheat VplB gene with ear germination resistance, and transform wheat by Agrobacterium-mediated method, Therefore, the wheat has ear germination resistance, and solves the problem that the existing wheat ear germination causes the decline of wheat yield and quality