Molecular marker closely linked with wheatear grain number major QTL and application of molecular marker

Abstract

The invention discloses a molecular marker closely linked with wheatear grain number major QTL. Wheat genome DNA serves as a template, a PCR primer pair is adopted to conduct PCR amplification, the amplificated product is subjected to ionophortic separation through polyacrylamide gel to obtain the molecular marker 2A-654918338. The invention further discloses application of the molecular marker 2A-654918338 in detecting whether a wheat variety or line contains the QTL for increasing the wheatear grain number and application of the molecular marker 2A-654918338 in molecular breeding of wheat. By means of the molecular marker, the genetic improvement progress of the wheatear grain number is accelerated, the selection efficiency and quality of the wheat variety or line are greatly improved, identification of the target gene in wheat genetic resources and breeding progenies is directly achieved, and more new wheatear grain number genetic resources are developed for wheat breeding.

Description

technical field [0001] The invention belongs to the field of wheat molecular biotechnology and breeding technology, and specifically relates to a molecular marker closely linked to the main effect QTL of wheat panicle number and its acquisition method and application. Background technique [0002] Wheat is the grain crop with the largest sowing area, the largest yield, and the widest distribution in the world, and it is also an important grain crop in my country. The three main factors that determine the yield of wheat are: the number of spikes per unit area, the number of grains per spike, and the thousand-grain weight. Among them, the impact of the number of grains per spike on yield is particularly prominent. Therefore, new genes related to increasing the number of grains per spike are excavated and functional molecular markers are developed. , which has important theoretical significance and practical application value for speeding up the breeding process of excellent wh...

AI Technical Summary

Problems solved by technology

[0003] The number of grains per spike in wheat is a quantitative trait regulated by multiple genes. The variation of these traits is continuous and greatly affected by the environment. The genetic basis is complex and the effect of a single gene is small. Therefore, it is difficult to study the genetics of the number of grains per spike.

The application of traditional quantitative genetics methods cannot effectively clarify the number of genes controlling the expression of traits, the location of genes on chromosomes, the effects of genes and their mode of action

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