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Early diagnosis and early warning kit for transplanted kidney rejection reaction and detection method

A technology for rejection and early diagnosis, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficulty, difficulty, and complicated diagnosis of transplant rejection

Inactive Publication Date: 2012-07-04
THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Transplant rejection is a very complex process, both cellular and humoral immune mechanisms are involved in this process, and there are many factors such as differences in grafts, the impact of surgery, individual differences in transplant recipients, and the application of different immunosuppressive regimens in clinical practice. Participation of allografts complicates and makes the diagnosis of transplant rejection more complicated and difficult
In addition, previous studies have shown that it is difficult to definitively diagnose transplant rejection by detecting a single molecule

Method used

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  • Early diagnosis and early warning kit for transplanted kidney rejection reaction and detection method
  • Early diagnosis and early warning kit for transplanted kidney rejection reaction and detection method
  • Early diagnosis and early warning kit for transplanted kidney rejection reaction and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1 Screening of Significant Difference Markers in Early Diagnosis Kit for Transplanted Kidney Rejection

[0105] 1. Collect serum of kidney transplant recipients at multiple time points after transplantation, and analyze and group clinical cases.

[0106] 1.1 Select kidney transplant recipients with preoperative basic diseases or few complications, and no serious complications during the operation, and select kidney transplant recipients at 1 day, 7 days, 14 days, 21 days, 28 days, and 2 months after transplantation. 1. Collect 2ml of whole blood from 3 months to 12 months, let stand at room temperature for 1 hour, centrifuge at 1000rpm for 10 minutes to collect the supernatant, store in -80°C refrigerator after aliquoting. In addition, the serum of the patients in the acute rejection group after the diagnosis of acute rejection was collected at any time.

[0107]1.2 According to the comprehensive analysis and case screening of serum creatinine, urea nitrogen, t...

Embodiment 2

[0123] The 35 markers with significant differences obtained in Example 1 were analyzed, and the markers with significant differences in expression levels were analyzed by logistic regression to obtain 4 factors: SCF, sEGFR, sIL2Ralpha and Eotaxin. The early warning and diagnosis system of acute rejection, the statistical analysis shows that the predicted positive rate is 91.4. The area under the ROC diagnostic curve is 97.5%, see attached figure 1 .

[0124] Wherein, the concrete composition of kit is:

[0125] (1) 96-well filter plate and two sealing membranes; purchased from Millipore Company, item number MX-PLATE;

[0126] (2) Standards of protein antibodies in the kit; purchased from Millipore Company, catalog numbers MXH8060, MXH8062, MXH8063, LHSP-8063 and HSCR-8032;

[0127] (3) Quality control control; purchased from Millipore Company, the article numbers are: MXH6060, MXH6062, MXH6063, LHSP-6063 and HSCR-6032;

[0128] (4) Serum matrix; purchased from Millipore Co...

Embodiment 3

[0135] Example 3: The composition of the kit for early diagnosis of acute rejection of transplanted kidney:

[0136] The 35 markers with significant differences obtained in Example 1 were analyzed, and 14 factors were obtained after logistic regression analysis on the markers with significant differences in expression levels: sTNFR2, Flt3Ligand, Fractalkine, IL1ra, IL2, MDC , MIP1alpha, SDF1alphabeta, TARC, TRAIL, SCF, CCL20, MIP3alpha and XCL1Lymphotactin; statistical analysis shows that the positive rate of early diagnosis system is 98.6%, and the area under the ROC diagnostic curve is 99.8%. figure 2 .

[0137] Wherein, the concrete composition of kit is:

[0138] (1) 96-well filter plate and two sealing membranes; purchased from Millipore Company, item number MX-PLATE;

[0139] (2) Standards of protein antibodies in the kit; purchased from Millipore Company, catalog numbers MXH8060, MXH8062, MXH8063, LHSP-8063 and HSCR-8032;

[0140] (3) Quality control control; purcha...

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Abstract

The invention relates to an early diagnosis kit and an early warning kit, in particular to an early diagnosis and early warning kit for transplanted kidney rejection reaction and a use method of the kit, and further to some proteins applied in the early warning of transplanted kidney acute rejection reaction or transplanted kidney rejection reaction. By applying a liquid protein chip (luminex) detection technique, the expression level of 96 cytokines / chemokines and receptors thereof in 266 serum samples from 142 patients having acute transplanted kidney rejection reaction and transplant recipients with stable function of transplanted kidneys are monitored and comprehensively analyzed. An early warning system for the transplanted kidney rejection reaction comprising at least one combined marker and an early diagnosis system for the transplanted kidney rejection reaction comprising at least one factor combined marker are established. The prediction and accuracy rate of the acute rejection achieve 98.6%, and the internal cross validation rates are 97.1% and 97.2%, respectively.

Description

technical field [0001] The present invention relates to an early diagnosis kit and an early warning kit, in particular, to an early diagnosis and early warning kit and detection method for transplanted kidney rejection, and also relates to some early warning kits for transplanted kidney rejection or transplanted kidney rejection. Applied proteins in early warning. Background technique [0002] The number of people undergoing dialysis due to chronic renal failure in the world has increased from 426,000 in 1990 to 1.065 million in 2000, and is expected to reach more than 2 million by 2010. This increase in the number of people has led to a rapid increase in global dialysis costs, which has caused a huge economic burden on both developed and developing countries. There are about 1 million uremia patients in my country, 120,000 new patients are added every year, and about 500,000 patients need kidney transplantation every year. At present, my country has carried out a total of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 石炳毅许晓光黄海燕
Owner THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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