Pathogen nucleic acid-drug resistant gene detection kit and application thereof

A gene detection and drug resistance technology, applied in the field of medicine and biology, can solve the problems of no mycoplasma resistance detection reagents, multiple target genes or pathogen detection, etc., and achieve rapid and objective detection results, strong repeatability and sensitivity high effect

Active Publication Date: 2015-04-15
常州百代生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the products currently on the market are single-channel detection, which can only perform single-plex PCR reactions. One PCR reaction tube can only detect one gene or pathogen in one reaction, and cannot specifically detect multiple target genes or pathogens, and Mycoplasma resistance detection reagents without clinically relevant methods

Method used

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  • Pathogen nucleic acid-drug resistant gene detection kit and application thereof
  • Pathogen nucleic acid-drug resistant gene detection kit and application thereof
  • Pathogen nucleic acid-drug resistant gene detection kit and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0065] The kit includes the following components:

[0066] PCR reaction solution, enzyme mixture, Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis / drug resistance gene multiple reaction solution, positive control 1, negative control;

[0067] The PCR reaction solution includes 10× buffer, 25mM MgCl2, 10mM dUTP and 10mM dNTPs;

[0068] The enzyme mixture includes Taq enzyme and UNG enzyme, the Taq enzyme is a hot start Taq enzyme, and the UNG enzyme is uracil-N-glycosylase.

[0069] Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis / drug resistance gene multiplex reaction solution upstream and downstream primers, and the ratio of the probe is: 6:4:2; the preferred upstream primer is 600nM, the downstream primer is 400nM, the probe is 200nM.

[0070] The nucleotide sequences of primers and probes of Mycoplasma hominis, Ureaplasma urealyticum, Chlamydia trachomatis and Ureaplasma urealyticum tetracycline resistance gene used in this example can be ...

Embodiment 2

[0110] The kit includes the following components:

[0111] PCR reaction solution, enzyme mixture, Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis multiplex reaction solution, drug resistance gene reaction solution, positive control 1, positive control 2, negative control;

[0112] The PCR reaction solution includes 10× buffer, 25mM MgCl2, 10mM dUTP and 10mM dNTPs;

[0113] The enzyme mixture includes Taq enzyme and UNG enzyme, the Taq enzyme is a hot start Taq enzyme, and the UNG enzyme is uracil-N-glycosylase.

[0114] Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis multiplex reaction solution has a ratio of upstream and downstream primers and probes of 6:4:2; the preferred upstream primer is 600nM, the downstream primer is 400nM, and the probe is 200nM.

[0115] The ratio of the upstream and downstream primers and probes of the drug resistance gene reaction solution is: 6:4:2; the preferred upstream primer is 600nM, the downstream primer is...

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Abstract

The invention relates to the technical field of medical biology, and especially relates to a pathogen nucleic acid-drug resistant gene detection kit and an application technology thereof. The kit designed by the invention includes the following two kinds of compositions: a PCR reaction buffer solution, an enzyme mixed solution, and a chlamydia trachomatis/mycoplasma urealytium/mycoplasma hominis/drug resistant gene multiple reaction solution; or a PCR reaction buffer solution, an enzyme mixed solution, a chlamydia trachomatis/mycoplasma urealytium/mycoplasma hominis multiple reaction solution, and a drug resistant gene reaction liquid. The kit adopts a multi-fluorescence quantitative PCR technology, can simultaneously detect multiple target genes in a same PCR reaction tube, and provides a powerful technical support for simultaneous detection of pathogens and drug resistant genes. A method has the characteristics of less required amount on samples, low cost, simple and convenient operation, high sensitivity and good specificity, and has extremely great social and economic significances.

Description

[0001] technical field [0002] The invention relates to the field of medical biotechnology, in particular to a detection kit for pathogen nucleic acid and drug resistance gene and its application, in particular to a kit for Chlamydia trachomatis / Ureaplasma urealyticum / Mycoplasma hominis and drug resistance gene and its application. application. Background technique [0003] Sexually transmitted diseases (STDs) are a group of worldwide infectious diseases that are primarily transmitted through sexual contact, although some STDs can be transmitted through non-sexual contact, such as through sharing needles, breastfeeding or even touching. [0004] At present, the incidence of STDs in my country is on the rise. Among them, urogenital diseases caused by three pathogens, Chlamydia trachomatis (CT), Mycoplasma hominis (M.Humenis, MH ) and Ureaplasma urealyticum (UU). Tract infection has become a common disease in the departments of gynecology, dermatology and venereal diseases, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/35C12R1/01
Inventor 孔祥宾夏国庆丁燕芬朱啸悦
Owner 常州百代生物科技股份有限公司
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