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Kit for rapidly detecting urogenital tract pathogens

A technology for urogenital tract and pathogens, which is applied in the field of kits for rapid detection of urogenital tract pathogens, can solve the problems of cumbersome and complicated sample nucleic acid processing process, high detection cost, and time-consuming detection, so as to meet the needs of clinical diagnosis and improve detection. Efficiency and easy operation

Inactive Publication Date: 2020-09-11
安徽同科生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] At present, there are kits for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae or Ureaplasma urealyticum by fluorescent quantitative PCR method on the market, but the sample nucleic acid processing process is cumbersome and time-consuming, and most of them are detection kits for single pathogens. Low throughput, high detection cost

Method used

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  • Kit for rapidly detecting urogenital tract pathogens
  • Kit for rapidly detecting urogenital tract pathogens
  • Kit for rapidly detecting urogenital tract pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: the composition of the kit of rapid detection genitourinary tract pathogen

[0054] The composition of the kit for rapid detection of urogenital pathogens is shown in Table 1:

[0055] Table 1 The composition of the kit for rapid detection of urogenital pathogens

[0056]

[0057]

[0058] Among them, the sample diluent is 5 mL of sterile saline;

[0059] Among them, the final concentration of each component in the PCR reaction solution is 1×PCR buffer, 100nM each primer, 100nM each probe, dNTPs (0.4mM dATP, 0.4mM dGTP, 0.4mM dCTP, 0.2mM dTTP, 0.2mM dUTP) , 4mM MgCl 2 ;

[0060] The primers and probes for Chlamydia trachomatis CT, for Neisseria gonorrhoeae NG, for U. urealyticum UU, and for human β-globin HBB are as follows:

[0061] Chlamydia trachomatis CT upstream primer CF: 5′-CCGTCGATCATAAGGCTTGGT-3′;

[0062] Chlamydia trachomatis CT downstream primer CR: 5′-AGTATTTGCCGCTTTGAGTTCTG-3′;

[0063] Chlamydia trachomatis CT probe CP: 5′-ATTCCC...

Embodiment 2

[0084] Embodiment 2: the use of the kit of rapid detection genitourinary tract pathogen

[0085] (1) Sample processing

[0086] Take 200 μL of the genitourinary secretion storage tube and transfer it to a 1.5 mL centrifuge tube, centrifuge at 12,000 rpm for 1 minute, remove the supernatant, add 100 μL of the sample suspension, mix well, and store at 2-8°C for later use.

[0087] (2) PCR detection

[0088] Take the corresponding amount of PCR reaction solution and Taq / UDG enzyme mixture in proportion (PCR reaction solution 43μL / per person+Taq / UDG enzyme mixture 2μL / person), mix well and then dispense into PCR reaction in 45μL / tube In a tube, set aside. Add 5 μL of the above-mentioned treated sample to the PCR reaction tube prepared with the PCR reaction solution, close the tube cap tightly. If there are air bubbles, flick with your fingers to remove the air bubbles, and centrifuge instantaneously until there are no obvious droplets on the tube wall.

[0089] (3) PCR amplific...

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Abstract

The invention provides a kit for rapidly detecting urogenital tract pathogens. The kit is characterized by comprising PCR reaction liquid, Taq / UDG enzyme mixture liquid, positive control and negativecontrol; the PCR reaction liquid comprises 10*PCR buffer, a primer and a probe of chlamydia trachomatis CT, a primer and a probe of gonococcus NG, a primer and a probe of ureaplasma urealyticum UU, aprimer and a probe of human beta-globin HBB, 25 mM MgCl<2>, dNTPs (dATP, dGTP, dCTP, dTTP, dUTP mixtures), and sterile distilled water. The kit in the invention has the advantages that: sample nucleicacid does not need to be extracted; detection is carried out after direct treatment; operation is simple, convenient and rapid; a single tube can detect multiple pathogens at the same time; the kit is economic and high-efficiency; high-flux sample detection can be carried out; the detection efficiency is increased; and the kit is good in specificity, high in pollution and interference resistance,good in result repeatability, and relatively precise.

Description

technical field [0001] The invention relates to a kit for rapidly detecting urogenital pathogens, belonging to the field of biological detection. Background technique [0002] In recent years, the number of urogenital tract infections has been increasing, and the pathogens causing infections are more complex, and there are certain asymptomatic infections, which make it difficult to diagnose and treat diseases. Among them, Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Ureaplasma urealyticum (UU) are the most common single or cross-infection clinically, and they are also the main pathogens of sexually transmitted diseases. [0003] Chlamydia trachomatis is a kind of chlamydia, which has filterability and is mainly transmitted through sexual contact. The clinical manifestations are often chronic. Many infected people have no obvious clinical manifestations, but they may cause serious sequelae. The clinical manifestations of male infection are mucoid or mucopurulen...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/01
CPCC12Q1/686C12Q1/689C12Q2563/107C12Q2545/114
Inventor 陈磊涛彭令苇李晓松宋冬梅
Owner 安徽同科生物科技有限公司
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