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Fluorescence enhanced two-color visual narcotic detection probe and preparation method thereof

A detection probe and fluorescence enhancement technology, applied in the field of public health and safety detection, can solve the problems of inability to achieve simultaneous detection of multiple drugs, insufficient detection sensitivity, and high signal-to-noise ratio of detection signals, achieving no need for professional skills and abundant raw material sources. , the effect of high detection sensitivity

Active Publication Date: 2019-12-24
ANHUI UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

However, in the field of drug detection, most of the current fluorescence detection methods are based on the fluorescence quenching mechanism. Therefore, the detection sensitivity is not high enough, the signal-to-noise ratio of the detection signal is high, and the accuracy is poor.
In addition, most fluorescent drug detection laws are aimed at a single type of drug, and cannot realize the simultaneous detection of multiple drugs

Method used

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  • Fluorescence enhanced two-color visual narcotic detection probe and preparation method thereof
  • Fluorescence enhanced two-color visual narcotic detection probe and preparation method thereof
  • Fluorescence enhanced two-color visual narcotic detection probe and preparation method thereof

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Embodiment 1

[0035] In this embodiment, the fluorescence-enhanced two-color visualized drug detection probe is prepared according to the following steps:

[0036] (1) Synthesis of polydopamine nanospheres

[0037] Add 0.1g of dopamine hydrochloride to a mixed solution of 60mL Tris buffer and 40mL isopropanol, and stir for 48 hours; after the reaction is completed, centrifuge, wash with ultrapure water for 3-5 times, and dry in a vacuum oven at 30°C for storage. That is, polydopamine nanosphere powder is obtained; in the mixed solution, the concentration of Tris buffer is 10 mM.

[0038] The absorption spectrum of gained polydopamine nanosphere powder is as follows figure 1 shown.

[0039] (2) Synthesis of copper nanoclusters

[0040]Synthesis of copper nanoclusters emitting orange fluorescence: Mix 360mmol copper nitrate and 9mol glutathione in 8mL ultrapure water, adjust the pH to 4 with 1.5M sodium hydroxide to obtain a mixed solution; The volume ratio of the solvent is 1:60, ethanol...

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Abstract

The invention discloses a fluorescence enhanced two-color visual narcotic detection probe and a preparation method thereof. The fluorescence enhanced two-color visual narcotic detection probe is composed of a polydopamine nanosphere and two copper nanoclusters emitting fluorescent lights in different colors under the action of a single exciting light, and a methylamphetamine monoclonal antibody and a ketamine monoclonal antibody are respectively modified on the surfaces of the two copper nanoclusters. The detection probe disclosed by the invention is simple in preparation method, low in cost,easy to use, high in sensitivity and strong in reliability and can realize synchronous visual detection on multiple narcotics.

Description

technical field [0001] The invention relates to the field of public health safety detection, in particular to a fluorescence-enhanced two-color visualized drug detection probe and a preparation method thereof. Background technique [0002] Drugs are a major problem that seriously threatens human health. Accurate drug detection is of great significance to combating drug crimes, detecting drug cases, and curbing the spread of drugs. In recent years, the soaring drug cases have also put forward higher requirements for drug detection technology. [0003] At present, commonly used drug detection techniques include traditional chromatography, spectroscopy, immunoassay, and electrochemical methods. Although these drug detection methods can meet the needs of anti-drug, anti-drug police and drug rehabilitation institutions in their work to a certain extent, these detection methods require expensive instruments, professional testers, and cumbersome operating steps, which undoubtedly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64B82Y15/00B82Y30/00
CPCG01N21/6428B82Y15/00B82Y30/00
Inventor 杜袁鑫李琳
Owner ANHUI UNIVERSITY
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