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86 results about "Treponema palidum" patented technology

Treponema pallidum is a spirochaete bacterium with subspecies that cause the diseases syphilis, bejel, and yaws. It is a helically coiled microorganism usually 6–15 µm long and 0.1–0.2 µm wide. The treponemes have a cytoplasmic and an outer membrane.

Methods, immunoassays and devices for detection of anti-lipoidal antibodies

The present application describes compositions, methods and devices for detecting anti-lipid antibodies and diagnosing diseases such as syphilis. In particular, methods for immobilizing lipid antigens (including cardiolipin, lecithin, and cholesterol) on solid supports (eg, nitrocellulose membranes) are described. The ability to immobilize lipid antigens on membranes fulfills a long known need for a membrane-based assay for the detection of anti-lipid antibodies. An immunoassay device for simultaneous treponema pallidum and non-treponemal testing is also described.
Owner:UNITED STATES OF AMERICA

Syphilis helicoid antibody chemiluminescence immune assay determination kit and method for preparing same

The invention discloses a kit of chemiluminescent immunological analysis measurement of a Treponema pallidum antibody, and preparation method thereof, which belongs to the technical field of immunological analysis medical diagnosis. The kit comprises (1) a carrier coated with a treponema pallidum specific recombination protein antigen; (2) treponema pallidum antibody negative and positive reference substances; (3) an enzyme labeled treponema pallidum specific recombination protein antigen; and (4) an enzyme acted chemiluminescent primer. Furthermore, the preparation method of the kit based on the invention comprises the following steps of (1) preparing TP antibody negative and positive reference substances; (2) labeling the TP specific recombination protein antigen with an enzyme; (3) coating with the carrier; (4) sub-packaging; and (5) assembling. The kit of the detection of the Treponema pallidum antibody has the advantages of easy and simple operation, easy popularization, high sensitivity, strong specificity, good repeatability, safety, non toxicity, and no pollution.
Owner:北京科美东雅生物技术有限公司

Rapidly dispersible vaginal tablet that provides a bioadhesive gel

InactiveUS20110159091A1Safe and relatively inexpensive methodAvoid spreadingAntibacterial agentsBiocideBacterial vaginosisSpiroplasma
A tablet for insertion into a vagina including 0.01 to 500 mg of a vaginal medication, such as a microbicide, such as cellulose acetate 1,2-benzenedicarboxylate (CAP); 100 to 500 mg of mannitol powder; 50 to 300 mg of inert microcrystalline cellulose; 10 to 80 mg of hydroxypropyl methylcellulose; 50 to 250 mg of glycerol and optionally 2 to 4 mg of at least one preservative which protects against microbicidal contamination and discourages the growth of yeast in the vagina. The tablet which includes CAP as the vaginal medication is vaginally administered before coitus in methods for preventing the sexual transmission of HIV-1, HIV-2, herpesvirus, or an infection caused by Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Haemophilus ducreyi or Treponema pallidum. The tablet which includes CAP as the vaginal medication is vaginally administered to prevent or treat bacterial vaginosis.
Owner:NEW YORK BLOOD CENT

Method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies

The invention belongs to the technical field of immunoassay and diagnosis and relates to a method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies. The method comprises the following steps of: using GoldMag particles as carriers, coating one or more antigens of specific recombinant proteins of treponema pallidum antibodies, TP15, TP17, TP47 and TP44.5, then adding a sample to be detected and enzyme labeled antigens, and finally adding luminescence substrates for luminescence detection. The GoldMag particle surface has a larger coupling capacity to the antigen and has characteristics of high specificity and no pollution of the enzyme linked immunoassay as well as high sensitivity of chemiluminescence, and therefore, the method has the advantages of high detection sensitivity, good specificity, wide linear range, no radioactive pollution and the like.
Owner:XIAN GOLDMAG NANOBIOTECH

Treponema pallidum antibody test kit and preparation method and detection method thereof

The invention relates to a treponema pallidum (TP) antibody test kit based on the flow microspheric carrier technology and a preparation method and detection method thereof, belonging to the technical field of immunoassay medical diagnosis. The preparation method comprises the following steps: using TP recombinant antigen to coat heavy polymer microsphere, using bovine serum albumin to seal empty binding site, preparing specific TP probe-heavy polymer microsphere; performing coculture with a sample to be tested to capture TP antibody, washing and centrifuging to remove the unbound TP antibody, then adding fluorescently-labeled anti-human IgG or IgM antibody; and using a flow cytometry to detect the fluorescence intensity of the microsphere, and performing qualitative or quantitative analysis to the tested antibody. The method has the advantages of high sensitivity and specificity and good stability and can be used to perform microanalysis or multivalent analysis to the sample.
Owner:NANJING UNIV OF TECH

Acquired immune deficiency syndrome virus and treponema pallidum fusion protein as well as preparation method and application thereof

The invention provides acquired immune deficiency syndrome virus and treponema pallidum fusion protein which can be used for preparing GICA (Gold Immunochromatographic Assay) one-step double-combined acquired immune deficiency syndrome and syphilis antibody rapid detection test paper, simultaneously and jointly detecting acquired immune deficiency syndrome and syphilis viruses, simultaneously carrying out combined detection on two diseases and carrying out result reading by naked eyes without special instruments, thereby providing a convenient, rapid, accurate and cheap detecting method for the site rapid detection, such as blood center street compulsory blood donor screening, family self-help detection, disease screening for frontier port exit and entry people, and the like, site epidemiology investigation and other mechanisms needing rapid detection.
Owner:中华人民共和国吉林出入境检验检疫局

Syphilis spirochete membrane antigen with shorten expression and uses thereof

InactiveCN101293919ASignificant antigen reactivityAntigen reactivity verificationBacteriaDepsipeptidesAntigenSpiroplasma
The invention discloses a DNA sequence expressing a truncated treponema pallidum membrane antigen and an amino acid sequence The membrane antigen is removed of the part having high homology with human fibronectin, so as to avoid false positive and improve the specificity of the serological test for diagnosis of treponema pallidum infection. The invention also discloses the application of the membrane antigen in preparing diagnostic reagents for detecting treponema pallidum infection.
Owner:ARMY MEDICAL UNIV

Kit for detecting treponema pallidum antibody as well as detection method and application thereof

ActiveCN104698185AAdequate binding reactionHigh detection sensitivityBiological testingAntigenMicrosphere
The invention discloses a kit for detecting a treponema pallidum antibody as well as a detection method and an application thereof, and belongs to the technical field of in vitro diagnosis and detection. The kit consists of the following components: (1) a marker system: including a treponema pallidum recombinant antigen 1 in direct or indirect connection to a marking tracer, or staphylococcus aureus A protein in direct or indirect connection to the marking tracker; (2) a bridged compound system: including a treponema pallidum recombinant antigen 2 in direct or indirect connection to a first bridged compound; and (3) a magnetic microsphere system: comprising a magnetic microsphere in direct or indirect connection to a second bridged compound, wherein the first bridged compound is combined with the second bridged compound. The kit and the detection method disclosed by the invention can promote the full implementation of conjugation reaction between antigen and antibody; and detection sensitivity can be improved.
Owner:SHENZHEN NEW INDS BIOMEDICAL ENG

Suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens

The invention relates to a suspension chip detection method for simultaneously detecting hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens. Plastic color coded microspheres with diameter of between 5.5 and 6.5 mu m are taken as vectors for a suspension chip; and the carriers are coated with monoclonal hepatitis B virus surface antibodies, HIV1, gP41 antigens, HCV NS3 antigens or treponema pallidum TP15 antigens. Through the suspension chip, the hepatitis B virus, hepatitis C virus, treponema pallidum and human immunodeficiency virus pathogens in a sample to be detected can be very conveniently and quickly detected at the same time; and the method has the advantages of accurate and reliable detection result, high specificity, small using amount of samples and high-throughput detection.
Owner:中华人民共和国陕西出入境检验检疫局

Treponema pallidum real-time fluorescence quantitative PCR (Polymerase Chain Reaction) multitarget detection kit and preparation thereof

The invention relates to a kit, particularly a Treponema pallidum real-time fluorescence quantitative PCR (Polymerase Chain Reaction) multitarget detection kit and preparation thereof. The kit comprises a DNA (deoxyribonucleic acid) extraction reagent bottle, a heat-resistant DNA polymerase bottle, a PCR reaction liquid bottle, a standard substance bottle, a quality control substance bottle and apacking box, wherein the DNA extraction reagent bottle comprises a protease K bottle, a lysis buffer bottle, an anhydrous alcohol bottle, an inhibitor removing liquid bottle, a first cleaning buffer bottle, an eluent bottle, a second cleaning buffer bottle and a centrifugation column containing a collection tube; the standard substance bottle is provided with 6 standard substance plasmid bottles containing 5 target genes; and the quality control substance bottle comprises a negative quality control substance bottle and a positive quality control substance bottle. The preparation method comprises the following steps: screening the target genes, and constructing the multitarget gene standard substance plasmids; after making a multitarget gene detection reagent standard curve, sequentially preparing PCR reaction liquid and heat-resistant DNA polymerase; after establishing a sample processing method, preparing the quality control substance; and finally, completing the Treponema pallidum real-time fluorescence quantitative PCR multitarget detection kit.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV

Full-automatic ELISA detection device for treponema pallidum on micro-fluidic chip and detection method thereof

The invention discloses a full-automatic ELISA detection device for treponema pallidum on a micro-fluidic chip. The full-automatic ELISA detection device comprises the micro-fluidic chip, a direct-current power source, a fluorescence detection system and a control system. The micro-fluidic chip comprises liquid storage tanks from first to seventh and a main channel, the liquid storage tanks from first to fifth are communicated with an inlet of the main channel through sample inlet channels, an outlet of the main channel is communicated with a sample outlet channel, and the other end of the sample outlet channel is communicated with the seventh liquid storage tank. The invention further discloses a detection method using the full-automatic ELISA detection device for treponema pallidum on the micro-fluidic chip. The full-automatic ELISA detection device has the advantages of being high in mechanization degree, simple in operation procedure, small in size, precise in structure, high in reliability and low in cost, and automatic control is convenient to achieve.
Owner:QIQIHAR MEDICAL UNIVERSITY

Method for detecting syphilis using synthetic antigens

An antigen composition and method for the detection of antibodies to Treponema pallidum and the diagnosis of syphilis are described. The antigen composition contains synthetic cardiolipin and synthetic lecithin. The antigen composition may additionally contain cholesterol and an alcohol. The antigen composition is useful as an immunoreagent in immunoassays for the detection of antibodies associated with T. pallidum infection. The methods are sensitive and specific for T. pallidum infection.
Owner:UNITED STATES OF AMERICA

Compositions and methods for detecting pathogen infection

The present invention generally features therapeutic and diagnostic compositions and methods for increasing or decreasing the binding of a lysozyme polypeptide to a Treponema pallidum P17 polypeptide (Tp17) or a Tp17-like polypeptide. More particularly, the invention relates to compositions and methods for detecting, treating, or preventing a pathogen infection or a chronic disorder; and to binding assays using a Tp17-like polypeptide and a lysozyme polypeptide.
Owner:BIOKIT +1

Method and kit for detecting treponema pallidum

A method for detecting luetin uses TP 0684 and TP 0453 syphilis specific antigens unitedly to make full use of characteristics and advantages of two said antigens for greatly raising sensitivity and specificity of detection. The kit for realizing said method to detect syphilis in early stage is also disclosed.
Owner:ARMY MEDICAL UNIV

Method for detecting syphilis using synthetic antigens

An antigen composition and method for the detection of antibodies to Treponema pallidum and the diagnosis of syphilis are described. The antigen composition contains synthetic cardiolipin and synthetic lecithin. The antigen composition may additionally contain cholesterol and an alcohol. The antigen composition is useful as an immunoreagent in immunoassays for the detection of antibodies associated with T. pallidum infection. The methods are sensitive and specific for T. pallidum infection.
Owner:UNITED STATES OF AMERICA

Ointment capable of quick sterilizing AIDS, veneral virus and spermatozoa and preparation process thereof

InactiveCN1371734AStop the spreadKeep your body and mind healthyHydroxy compound active ingredientsAerosol deliveryDiseaseAIDS Study
The bacteria-killing ointment capable of quickly-killing AIDS virus, VD virus and spermium is a colourless, smellless, water-soluble transparent paste-like liquid made of octylbenzopolyalcohol, aloe and zephiran chloride, etc. as main raw material through a certain special process. In the sexual intercourse it can inactivate AIDS virus in 15 min., can kill disease sources of various VD, such as gonococcus, treponema pallidum, vaginitis and trichlomonas vaginalis, etc. within 1 min. and and can kill spermium of human body in 1 min. It is safe and reliable.
Owner:吉安长江生物药业有限公司

Treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and preparation method thereof

The invention relates to a treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and a preparation method thereof, which relates to the syphilis detection. The kit comprises a concentrated washing liquid bottle, a negative and positive control bottle, a biotin mark antihuman IgG specific fragment gamma chain monoclonal antibody bottle, a horseradish peroxidase mark avidin bottle, a TMB coloured solution bottle A, a coloured solution bottle B, a reaction stopping solution bottle, a micropore plate coated with recombinant antigen. The treponema pallidum has infectious standard strain Nichol strain and treponema pallidum non-infectious standard strain Reiter strain, by using a proteomics method, the treponema pallidum proteomics can be separated through dimensional electrophoresis, protein with strong immunogenicity can be identified by different patient or infectious animal serum, and then an antigen marker can be searched. The antigen marker is used for syphilis confirmation test, so that diagnosis sensitivity and specificity can be increased, a window stage is shortened. The treponema pallidum antibody can be sensitively detected and enables quantitative measurement, and has the advantages of cheap price, simple operation and accurate result.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV

Preparation method and application of protein chip based on serological detection of syphilis

The invention relates to the field of biotechnical detection, and in particular relates to a protein chip for detecting syphilis as well as a preparation method and application thereof. A surface dot matrix of a solid-phase vector of the protein chip is fixed with a treponema pallidum recombinant antigen rTpN15-17-47; and the protein chip disclosed by the invention can be used for respectively detecting IgG and IgM antibodies in blood serums of patients suffering syphilis, is high in sensitivity, ensures that the visual detection limit is as low as 0.19mu g / mL and the positive rate can reach 99.0%, and ensures that both the visual detection limit and the positive rate are higher than those of conventional TRUST and TPPA detection methods.
Owner:ANHUI MEDICAL UNIV

Treponema pallidum p15-17-47 fusion protein and recombination expression method thereof

The invention relates to treponema pallidum p15-17-47 fusion protein and a recombination expression method thereof, and belongs to the technical field of mechanical diagnosis of immunoassay. According to the technical scheme, the recombination expression method of treponema pallidum p15-17-47 fusion protein comprises the following steps in sequence: respectively performing PCR amplification for gene segments of p15, p17 and p47 three protein fragments in treponema Nihcols strains; analyzing effective epitopes of the three protein segments; amplifying and performing enzyme-cut and link up for the effective segments; building recombinant plasmid pPIC9k-p15-17-47 and recombinant saccharomycetes eukaryotic expressing system GS115pPIC9k-p15-17-47; performing liquid culture for recombinant yeast; and purifying recombinant expression products.
Owner:GUANGZHOU KANGRUN BIOTECHNOLOGY CO LTD

Rapid and sensitive genotype identification and nucleic acid detection

The invention discloses a method, primer, probe and kit for identifying various gene mutations or substance genetic typing. In one embodiment, the disease is pathogenic. In the other embodiment, the invention is applied to detection of multidrug-resistant Mycobacterium tuberculosis, hepatitis B virus, beta-globulin mutation, thrombophilia related mutation; or various sexually transmitted pathogens, consisting of Trichomonas vaginalis, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, Treponema pallidum, Herpes simplex virus 1 , Herpes simplex virus 2, Human papillomavirus type 6, and Human papillomavirus type 1.
Owner:DIAGCOR LIFE SCI LTD

Treponema pallidum (TP) nucleic acid testing kit

A TP nucleic acid testing kit mainly comprises a polymerase chain reaction (PCR) reaction reagent and a DNA extraction reagent. The testing kit is characterized in that the PCR reaction reagent comprises an upstream outer primer of specifically amplified TP nucleic acid, a downstream outer primer of the specifically amplified TP nucleic acid, a probe for detecting the TP nucleic acid, wherein the base sequence of the upstream outer primer is 5'-GGTCGATGTGCAAATGAGTGTT-3; The base sequence of the downstream outer primer is 5'-CCGACTTCAATACGGAGTTCAC-3'; the base sequence of the probe is 5'-ACTAGCCCTCCCTTCTACCTGAGATAAG-3'; and the 5'end of the probe is marked through fluorescent dye, and the 3'end of the probe is marked through quenching fluorescent dye. The testing kit has the advantages of being high in sensitivity, specificity and throughput, capable of detecting the TP rapidly and accurately, and suitable for clinical application and the like.
Owner:泰普生物科学(中国)有限公司

Treponema pallidum triplet antigen

A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.
Owner:ORTHO-CLINICAL DIAGNOSTICS

LAMP primer combination for detecting three ophthalmic infection spirochetes and application

The invention discloses an LAMP primer combination for detecting three ophthalmic infection spirochetes and application. The primer combination is composed of eighteen single-stranded DNA molecules shown from the sequence 1 to the sequence 18. The invention further provides application of the primer combination. The invention furthermore provides a method of using the primer combination for identifying whether a spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira, a method for identifying whether the spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira and a method for identifying whether a sample to be detected is infected by treponema pallidum and / or borrelia burgdorferi and / or leptospira. Treponema pallidum, borrelia burgdorferi and leptospira can be fast and accurately detected by using the method.
Owner:智德科技(无锡)有限公司

Fluorescent PCR detection kit for detecting treponema pallidum

The invention provides a fluorescent PCR detection kit for detecting treponema pallidum. The kit comprises a nucleic acid releasing agent and a PCR reaction solution containing sequences of a primer and a probe which are used for detecting target nucleotide, wherein the nucleic acid releasing agent comprises 0.01-0.5mmol / L of surfactin, 50-200mmol / L of potassium chloride and 0.01%-2% by mass of sodium dodecyl sulfate and 0.05%-1% by volume of ethanol; the sequence of the primer for detecting target nucleotide comprises: an upstream primer: 5'-GACTGACCCAAGCGTTACTAAGATG-3', a downstream primer: 5'-CCCTCGACTGCTTCAAACTTAATC-3', and the sequence of the probe is 5'-CCCCGTCCTCTCCCAAATCCTGA-3'. When the fluorescent PCR detection kit for detecting treponema pallidum, which is provided by the invention, is used for detecting unknown samples such as skin lesion exudates and genital tract secretions, the operation is rapid, the method is simple, the detection sensitivity is high and the detection range is wide.
Owner:SANSURE BIOTECH INC

Syphilis test strip and preparation method thereof, and syphilis detection card and preparation method thereof

The invention relates to the technical field of immunodetection, and particularly relates to a syphilis test strip and a preparation method thereof, and a syphilis detection card and a preparation method thereof. The syphilis test strip comprises a bottom plate (1). The bottom plate (1) is provided with a sample pad (2), a glass cellulose membrane (3), a nitrocellulose membrane (4) and absorbent paper (5) in sequence. The glass cellulose membrane (3) is provided with a colloidal gold-marked treponema pallidum specific antigen and a colloidal gold-marked cardiolipin-phospholipid binding protein complex. A preparation method of the colloidal gold-marked cardiolipin-phospholipid binding protein complex comprises the steps of mixing cardiolipin and phospholipid binding protein; carrying out first incubation to obtain the cardiolipin-phospholipid binding protein complex; mixing the cardiolipin-phospholipid binding protein complex and colloidal gold; carrying out second incubation; and sealing to obtain the colloidal gold-marked cardiolipin-phospholipid binding protein complex. The detection card provided by the invention is simple and convenient to operate, has high sensitivity, high specificity and low false positive rate, and prevents interference of previous infection in current infection.
Owner:河南泰宇高分医疗器械有限公司 +2

Rapid detective reagent strip of treponema pallidum immunoglobulin m (IgM) antibody and preparation method thereof

The invention discloses a rapid detective reagent strip of treponema pallidum immunoglobulin m (IgM) antibody. The rapid detective reagent strip of the treponema pallidum IgM antibody comprises an upper sampling pad (1), a colloidal gold pad (2), a NC film (3), an absorbing sampling pad (4) and a plastic plate (7). The colloidal gold pad (2) is marked with a T1 antibody and a TP fusion protein. An upper peridium man-resistant IgM mu chain monoclonal antibody of the NC film (3) forms a T line (5) and a sheep-resistant-mouse immunoglobulin G (IgG) antibody forms a C line (6). The upper sampling pad (1), the colloidal gold pad (2), the NC film (3) and the absorbing sampling pad (4) are pasted on the plastic plate (7) to form a detective reagent strip. Compared with the prior art, the rapid detective reagent strip of the treponema pallidum IgM antibody has the advantages of being high in sensitivity, strong in specificity and easy and convenient to operate. The rapid detective reagent strip of the treponema pallidum IgM antibody can provide an exact experimental basis for observation of curative effects after a clinical early stage diagnosis, an early treatment and an anti-luetic treatment.
Owner:天津中新科炬生物制药股份有限公司

Rapid genotyping analysis and kits thereof

The present invention provides methods, primers, probes, and kits for identifying gene mutation or material genotyping. In one embodiment, the materials are disease-causing agents. In another embodiment, the present invention is applied to detecting multidrug-resistant mycobacterium tuberculosis, hepatitis B virus, beta-globulin mutation, thrombosis-related mutations; or multiple sexually transmitted diseases causing agents, including but not limited to, trichomonas vaginalis, chlamydia, neisseria gonorrhoeae, mycoplasma, mycoplasma hominis, ureaplasma urealyticum, Mycelia, Treponema pallidum, Herpes simplex virus type 1 and type 2 and human papilloma virus type 6 and type 11.
Owner:DIAGCOR LIFE SCI LTD

High-throughput treponema pallidum specific antibody detection kit and preparation method thereof

ActiveCN104330562AAccurate detectionAccurate Epidemiological SurveyMaterial analysisSpiroplasmaSpecific igm
The invention provides a high-throughput treponema pallidum specific antibody detection kit and a preparation method thereof, and relates to treponema pallidum. The kit comprises an outer packaging box, an alkaline phosphatase labeled antihuman gamma monoclonal antibody bottle, an alkaline phosphatase labeled antihuman mu monoclonal antibody bottle, an alkaline phosphatase labeled antihuman Ig monoclonal antibody bottle, a chemiluminescent substrate bottle, a treponema pallidum specific antibody negative reference substance bottle, a treponema pallidum specific IgG antibody positive reference substance bottle, a treponema pallidum specific IgM positive reference substance bottle, a treponema pallidum specific total antibody positive reference substance bottle, a washing liquid bottle and a recombinant antigen coated microporous plate. The preparation method of the kit comprises the steps of firstly preparing a treponema pallidum specific recombinant antigen, the recombinant antigen coated microporous plate, an antihuman gamma chain monoclonal antibody, an antihuman mu chain monoclonal antibody and an antihuman Ig monoclonal antibody, labelling the alkaline phosphatases of the antibodies, and then preparing a chemiluminescent substrate, washing liquid and reference substances, and finally, assembling the high-throughput treponema pallidum specific antibody detection kit.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
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