The invention relates to a kit, particularly a
Treponema pallidum real-time
fluorescence quantitative PCR (
Polymerase Chain Reaction) multitarget detection kit and preparation thereof. The kit comprises
a DNA (deoxyribonucleic acid) extraction
reagent bottle, a heat-resistant
DNA polymerase bottle, a PCR reaction liquid
bottle, a standard substance bottle, a
quality control substance bottle and apacking box, wherein the
DNA extraction
reagent bottle comprises a
protease K bottle, a
lysis buffer bottle, an
anhydrous alcohol bottle, an inhibitor removing liquid bottle, a first cleaning buffer bottle, an eluent bottle, a second cleaning buffer bottle and a
centrifugation column containing a collection tube; the standard substance bottle is provided with 6 standard substance
plasmid bottles containing 5 target genes; and the
quality control substance bottle comprises a negative
quality control substance bottle and a positive quality control substance bottle. The preparation method comprises the following steps: screening the target genes, and constructing the multitarget
gene standard substance plasmids; after making a multitarget
gene detection
reagent standard curve, sequentially preparing PCR reaction liquid and heat-resistant
DNA polymerase; after establishing a
sample processing method, preparing the quality control substance; and finally, completing the
Treponema pallidum real-time
fluorescence quantitative PCR multitarget detection kit.