36 results about "Treponema pallidum antibody" patented technology

It is an object of the present invention to provide a method for preventing serum interference for assaying anti-Treponema pallidum antibodies and for improving storage stability of reagent of anti-phospholipid antibodies, which is excellent in long-term storage stability and enables an accurate diagnosis of syphilitic infection by preventing an occurrence of serum interference. The step of assaying anti-Treponema pallidum antibodies comprises a process that a sample is contacted with the anti-Treponema pallidum antibody reagent having an insoluble carrier supporting an anti-Treponema pallidum antigen thereon, a process that the insoluble carrier supporting an anti-Treponema pallidum antigen thereon is reacted with the anti-Treponema pallidum antibody in the sample, and a process that agglutination level of the antigen-antibody reaction is determined by optical measurement or visual observation, wherein the anti-Treponema pallidum antibody assaying reagent is added with a copolymer having a segment derived from 2-methacryloyloxyethyl phosphorylcholine and a segment derived from a hydrophilic monomer.

The invention discloses a method and a device for judging the detection result of a treponema pallidum antibody. The method comprises the following steps: performing agglutination reaction on serum or plasma of a to-be-detected patient by the treponema pallidum antibody and performing reaction on the serum or plasma of the to-be-detected patient without the treponema pallidum antibody separately by a treponema pallidum gelatin agglutination test method to acquire respective sample reaction plates; obtaining pictures of a to-be-detected sample and the control sample reaction plates; performing imaging processing on the pictures to acquire a plurality of pixel points and corresponding agglutination color pixel values from the pictures, and converting the pictures into two-dimensional data for storage; comparing parameters, such as shape, peak value, ratio of peak height to peak width, peak valley, difference value between peak height and peak valley and agglutination ring size, of agglutination color gray value curves of a to-be-detected group and a control group with a standard threshold value to judge the detection result. According to the method and the device for judging the detection result of the treponema pallidum antibody, the detection result of the treponema pallidum antibody can be judged more accurately and the detection efficiency is improved.

The invention discloses a kit for detecting a treponema pallidum antibody as well as a detection method and an application thereof, and belongs to the technical field of in vitro diagnosis and detection. The kit consists of the following components: (1) a marker system: including a treponema pallidum recombinant antigen 1 in direct or indirect connection to a marking tracer, or staphylococcus aureus A protein in direct or indirect connection to the marking tracker; (2) a bridged compound system: including a treponema pallidum recombinant antigen 2 in direct or indirect connection to a first bridged compound; and (3) a magnetic microsphere system: comprising a magnetic microsphere in direct or indirect connection to a second bridged compound, wherein the first bridged compound is combined with the second bridged compound. The kit and the detection method disclosed by the invention can promote the full implementation of conjugation reaction between antigen and antibody; and detection sensitivity can be improved.

The invention aims to provide a reagent for assaying an antiphospholipid antibody which is excellent in long-term storage stability and capable of diagnosing syphilis infection or the like and a reagent for assaying an anti-Treponema pallidum antibody which is capable of diagnosing syphilis infection with high accuracy by preventing the occurrence of serum interference. The invention is directed to the reagent for assaying an antiphospholipid antibody to be used for diagnosing syphilis infection comprising an insoluble carrier carrying an antiphospholipid antigen and a copolymer with a segment derived from 2-methacryloyloxyethyl phosphorylcholine and a segment derived from a hydrophilic monomer.

The invention relates to a treponema pallidum IgM antibody biotin avidin enzyme-linked immune detection kit and a preparation method thereof, which relates to the syphilis detection. The kit comprises a concentrated washing liquid bottle, a negative and positive control bottle, a biotin mark antihuman IgM specific fragment mum chain monoclonal antibody bottle, a horseradish peroxidase mark avidin bottle, a TMB coloured solution bottle A, a coloured solution bottle B, a reaction stopping solution bottle, a micropore plate coated with recombinant antigen. The treponema pallidum has infectious standard strain Nichol strain and treponema pallidum non-infectious standard strain Reiter strain, by using a proteomics method, the treponema pallidum proteomics can be separated through dimensional electrophoresis, protein with strong immunogenicity can be identified by different patient or infectious animal serum, and then an antigen marker can be searched. The antigen marker is used for syphilis confirmation test, so that diagnosis sensitivity and specificity can be increased, a window stage is shortened. The treponema pallidum antibody can be sensitively detected and enables quantitative measurement, and has the advantages of cheap price, simple operation and accurate result.

The present invention relates to a recombinant chimeric antigen of Treponema pallidum, which consists of the amino acid sequence shown in SEQ ID NO.12, the first flexible linker, the amino acid sequence shown in SEQ ID NO.9, the second flexible linker It is formed in series with the amino acid sequence shown in SEQ ID NO.13. The recombinant chimeric antigen of the invention can be used for serological detection of treponema pallidum antibody, and has improved dissolution expression ability, activity and thermal stability. The present invention also relates to a method for preparing the recombinant chimeric antigen.