The invention discloses a method for increasing the soluble expression quantity of 4, 6-alpha-glucosyltransferase, belongs to the technical field of protein engineering and fermentation engineering, and particularly relates to a method for increasing the soluble expression quantity of 4, 6-alpha-glucosyltransferase (GtfB) by adding betaine, maltose, beta-cyclodextrin, trehalose and sorbitol smallmolecular substances into a fermentation medium in a process of expressing 4, 6-alpha-glucosyltransferase (GtfB) derived from lactobacillus fermentum by respectively taking escherichia coli and bacillus subtilis as hosts. The total enzyme activity of fermentation is improved. An effective strategy is provided for efficient expression of 4, 6-alpha-glucosyltransferase (GtfB), the operation processis simple, the cost is low, the effect is remarkable, and a foundation is laid for industrial production.