Diagnosis reagent for syphilis spirochete antibody
A technology of Treponema pallidum and diagnostic reagents, which is applied in the field of Treponema pallidum antibody enzyme-labeled diagnostic reagents, can solve problems such as clinical judgment errors, burdens, and increased workload, and achieve standardized management assurance, good stability, and stable reagents.
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Embodiment 1
[0011] Reagent composition:
[0012] 1. Use synthetic peptide antigen or antigen expressed by genetic engineering: P15, P17, P47 antigen 1μg / ml coated on the microplate.
[0013] 2. Enzyme-labeled secondary antibody (IgG, IgM): containing 1:35K enzyme-labeled secondary antibody dilution solution
[0014] 3. Two-in-one color developer:
[0015] 0.05 parts of TMB sodium propanesulfonate;
[0016] 0.05 part of cumene hydroperoxide;
[0017] 10 parts of N-methyl-2-pyrrolidone;
[0018] 10 parts of ethanol;
[0019] 0.05 parts of ProClin300 (2-methyl-4-isothiazolin-3-one);
[0020] Piperazine-N, N'-bis(2-ethanesulfonic acid) buffer solution 20mmol / L pH7.0.
Embodiment 2
[0022] Reagent composition:
[0023] 1. Coating with synthetic peptide antigen or antigen expressed by genetic engineering: P15, P17, P47 antigen 1 g / ml
[0024] on a microtiter plate.
[0025] 2. Enzyme-labeled secondary antibody (IgG, IgM): containing 1:35K enzyme-labeled secondary antibody dilution solution
[0026] 3. Two-in-one color developer:
[0027] 0.05 parts of TMB sodium propanesulfonate;
[0028] Hydroxyurea 0.05 part;
[0029] Hydroxypropyl cyclodextrin-5mmol / L;
[0030] 10 parts of dimethyl sulfoxide;
[0031] 0.05 parts of ProClin300 (2-methyl-4-isothiazolin-3-one);
[0032] Piperazine-N, N'-bis(2-ethanesulfonic acid) buffer solution 20mmol / L pH7.0.
Embodiment 3
[0057] Reagent composition:
[0058] 1. Use synthetic peptide antigen or antigen expressed by genetic engineering: P15, P17, P47 antigen 1μg / ml coated on the microplate.
[0059] 2. Enzyme-labeled secondary antibody (IgG, IgM): containing 1:35K enzyme-labeled secondary antibody dilution solution
[0060] 3. Two-in-one color developer:
[0061] 0.5 parts of TMB sodium propanesulfonate;
[0062] Hydroxyurea 0.01 part;
[0063] 50% parts of N-methyl-2-pyrrolidone;
[0064] 0.5 parts of ProClin300 (2-methyl-4-isothiazolin-3-one);
[0065] Piperazine-N, N'-bis(2-ethanesulfonic acid) buffer solution 100mmol / L pH8.0.
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