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Kit for detecting treponema pallidum antibody in urine, preparation method and use method

A technology for detecting Treponema pallidum and antibodies, which is applied in biological tests, measuring devices, immunoassays, etc., can solve the problems that the sensitivity of reagents is difficult to reach the detection level, the content of Treponema pallidum antibodies is low, etc., and achieves improved sensitivity of reagents, high recovery rate, and easy operation. simple effect

Pending Publication Date: 2020-09-08
郑州方欣生物科技有限责任公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Urine sampling is convenient, safe, and non-invasive, but the content of Treponema pallidum antibodies in urine is very low, and the sensitivity of the reagents is difficult to reach the detection level

Method used

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Embodiment Construction

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] The invention provides a technical solution: a test kit for detecting Treponema pallidum antibodies in urine, including a detection test strip, the detection test strip consists of a sample pad fixed on a PVC board, a binding pad, a coated detection line and a quality control The combination pad is coated with gold standard SPA, the nitrocellulose membrane detection line is coated with Treponema pallidum recombinant antigen, and the quality control line is coated with rabbit anti-SPA.

[0024] The preparation method of the Tr...

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Abstract

The invention discloses a kit for detecting a treponema pallidum antibody in urine, a preparation method and a use method. The kit comprises a detection test strip. The detection test strip comprisesa sample pad, a combination pad, a nitrocellulose membrane, absorbent paper and a PVC bottom plate, wherein the sample pad, the combination pad, the nitrocellulose membrane and the absorbent paper aresequentially adhered to the PVC bottom plate; the combination pad is coated with a recombinant protein A (SPA)-colloidal gold marker, and the nitrocellulose membrane is respectively coated with a detection line composed of TP recombinant antigen and a quality control line composed of rabbit anti-SPA. Besides, a urine sample is concentrated through a concentration medium, so that the content of the treponema pallidum antibody in urine is increased, and the sensitivity of the kit for detecting the treponema pallidum antibody in the urine is improved. The detection sensitivity can be improved by2 to 6 times. While the kit is used, the urine is used as a detection sample, and sampling operation can be conducted in asafe, simple, convenient and noninvasive manner. And the kit is high in sensitivity, strong in specificity, convenient to operate and suitable for detection under various conditions.

Description

technical field [0001] The invention relates to the technical field of detection of Treponema pallidum antibodies in urine, in particular to a detection kit for Treponema pallidum antibodies in urine, a preparation method and a use method. Background technique [0002] Syphilis is a chronic sexually transmitted disease caused by Treponema pallidum infection. It is mainly transmitted directly through sexual contact. It can also be transmitted by kissing, surgery, breastfeeding, blood transfusion and contact with pollutants. It can invade multiple systems and organs, and its clinical symptoms are complex. Easily missed and misdiagnosed. The laboratory diagnostic methods of syphilis mainly fall into three categories: molecular biology diagnosis, etiological diagnosis and serological diagnosis. Due to the high requirements of experimental conditions, the first two methods are limited to scientific research units and teaching institutions, while serological diagnosis is widely us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/58
CPCG01N33/56911G01N33/558G01N33/587G01N2333/20G01N2469/20
Inventor 詹利红申庆红杨卫平李美霞张培蕾
Owner 郑州方欣生物科技有限责任公司
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