Reagent for inspecting syphilis spiral antibody by magnetic agglutination method

A technology for Treponema pallidum and antibody detection, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of false negative results, high price, and complicated operation.

Inactive Publication Date: 2007-11-21
长春博德生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently routinely used syphilis confirmatory test is more complicated to operate, takes a long time, requires specialized equipment, and is relatively expensive
Recently, the PCR principle is used to detect syphilis. Its sensitivity and specificity are very good. Treponema pallidum can be detected in the early stage, second stage, and third stage of syphilis, and it does not cross-react with other spirochetes, but a small amount of pollution can be obtained. False-positive results are also easily inhibited by substances such as tissues and cells in the specimen, resulting in false-negative results, so it has not been popularized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] NaOH 2.5 HCl 0.25

[0048] FeCl 2 4H 2 O 1.49 FeCl 3 ·6H 2 O 3.5

[0049] (1) Preparation of molten iron: weigh FeCl 2 4H 2 O and FeCl 3 ·6H 2 O, dissolve in the same container with HCl solution, and dilute to volume with HCl solution after filtration;

[0050] (2) Preparation of magnetic particles: Under room temperature conditions, nitrogen source, ventilation pipe, reactor and stirrer are installed, NaOH is added into the reactor, and nitrogen is continuously fed into the reactor, and the stirrer is started at 10000 per minute. Stir at a constant speed, add molten iron into the reactor at a constant speed along the container wall, stir at room temperature for 50 minutes, then turn off the power of the agitator and the nitrogen valve to end the reaction; move the liquid in the reactor to the Erlenmeyer flask, add The liquid in the bottle is equal to the volume of purified water, act in a magnetic field until the liquid is clarified and the magnetic particles ...

Embodiment 2

[0052] NaOH 3 HCl 0.29

[0053] FeCl 2 4H 2 O 1.94 FeCl 3 ·6H 2 O 4.05

[0054] The preparation method is the same as in Example 1.

Embodiment 3

[0056] NaOH 3.5 HCl 0.35

[0057] FeCl2 4H2O 2.5 FeCl3 6H2O 4.6

[0058] The preparation method is the same as in Example 1.

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PUM

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Abstract

A reagent using magnetic agglutination method to detect antibody of syphilis spire is prepared as enveloping recombined syphilis antigen on surface of magnetic particle then using magnetic agglutination method to carry out detection on antibody of syphilis spire and forming said magnetic particle by NaOH, HCI, FeCI2.4H2O and FeCI3 .6H2O.

Description

Technical field: [0001] The invention relates to the detection of Treponema pallidum antibody in human serum. Background technique: [0002] Syphilis is a sexually transmitted disease with extremely complex clinical manifestations, which can affect almost every organ of the body, and can be transmitted to the next generation through the placenta. Because syphilis is seriously harmful to humans, it is regarded as an important social and medical problem all over the world. highly valued. In addition, distinguishing syphilis-positive patients from patients with false-positive results caused by experiments in time can block patients' psychological pressure and blind treatment. [0003] Serological antibody detection is currently the main basis for finding and diagnosing syphilis cases in various countries. Routine serological detection of syphilis includes two steps of primary screening and confirmation. Preliminary screening tests (such as VDRL, RPR, TRUST, etc.) are non-spe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571
Inventor 李勇隋宝珍
Owner 长春博德生物技术有限责任公司
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